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Volume 66, Issue 5

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Endothelial cell adhesion on polyelectrolyte multilayer films functionalised with fibronectin and collagen

Nahla Zanina
  • Biophysic Laboratory, Faculty of Medicine, University of Monastir, 5019, Monastir, Tunisia
  • Inserm, U698, Department of Bioengineering, Institute Galilée, Paris 13 University, 99 av. J. B. Clement, 93430, Villetaneuse, France
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 / Soumaya Haddad
  • Biophysic Laboratory, Faculty of Medicine, University of Monastir, 5019, Monastir, Tunisia
  • Inserm, U698, Department of Bioengineering, Institute Galilée, Paris 13 University, 99 av. J. B. Clement, 93430, Villetaneuse, France
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 / Ali Othmane / Thierry Jouenne
  • CNRS UMR 6270 & FR 3038-Proteomic platform of the European Institute for Peptide Research (IFRMP23), University of Rouen, 3 av. Pasteur, 76821, Mont saint-Aignan, Rouen, France
  • Inserm U413, Laboratory of Cellular and Molecular Neuroendocrinology, U413, University of Rouen, 3 av. Pasteur, 76821, Mont saint-Aignan, Rouen, France
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 / David Vaudry
  • Inserm U413, Laboratory of Cellular and Molecular Neuroendocrinology, U413, University of Rouen, 3 av. Pasteur, 76821, Mont saint-Aignan, Rouen, France
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 / Mina Souiri / Laurence Mora
  • Inserm, U698, Department of Bioengineering, Institute Galilée, Paris 13 University, 99 av. J. B. Clement, 93430, Villetaneuse, France
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Published Online: 2012-04-05 | DOI: https://doi.org/10.2478/s11696-012-0141-7

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Abstract

The seeding of endothelial cells on biomaterial surfaces has become a major challenge to achieve better haemocompatibility of these surfaces. Multilayers of polyelectrolytes formed by the layerby-layer method are promising in this respect. In this study, the interactions of endothelial cells with multilayered polyelectrolytes films were investigated. The build-ups were prepared by selfassembled alternatively adsorbed polyanions and polycations functionalised with fibronectin and collagen. Anionic poly(sodium 4-styrenesulfonate) and cationic poly(allylamine hydrochloride) polyelectrolytes were chosen as a model system. Elaborated surfaces were characterised by electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode showed good reversible electrochemical properties and high stability in an electrolyte solution. The film ohmic resistance was highest when the film was coated with fibronectin; the parameters so determined were correlated with atomic force microscopy images. Cell colorimetric assay (WST-1) and immunofluorescence were used to quantify the cell viability and evaluate the adhesion properties. When cultured on a surface where proteins were deposited, cells adhered and proliferated better with fibronectin than with collagen. In addition, a high surface free energy was favourable to adhesion and proliferation (48.8 mJ m−2 for fibronectin and 39.7 mJ m−2 for collagen, respectively). Endothelial cells seeded on functionalised-polyelectrolyte multilayer films showed a good morphology and adhesion necessary for the development of a new endothelium.

Keywords: polyelectrolyte multilayers; endothelial cells; cell adhesion; proliferation; atomic force microscopy; wettability; electrochemical impedance spectroscopy

  • [1] Albelda, S. M. (1993). Role of integrins and other cell adhesion molecules in tumor progression and metastasis. Laboratory Investigation, 68, 4–17. Google Scholar

  • [2] Bery, M. N., & Grivell, M. B. (1995). An electrochemical description of metabolism. In D. Walz, H. Berry, & G. Milazzo (Eds.), Bioelectrochemistry of cells and tissues (pp. 134–158). Basel, Switzerland: Birkhauser Verlag. http://dx.doi.org/10.1007/978-3-0348-9063-2_4Google Scholar

  • [3] Borghol, N., Mora, L., Sakly, N., Lejeune, P., Jouenne, T., Jaffrézic-Renault, N., & Othmane, A. (2011). Electrochemical monitoring of chlorhexidine digluconate effect on polyelectrolyte immobilized bacteria and kinetic cell adhesion. Journal of Biotechnology, 151, 114–121. DOI: 10.1016/j.jbiotec.2010.11.014. http://dx.doi.org/10.1016/j.jbiotec.2010.11.014CrossrefWeb of ScienceGoogle Scholar

  • [4] Bouafsoun, A., Othmane, A., Kerkeni, A., Jaffrézic, N., & Ponsonnet, L. (2006). Evaluation of endothelial cell ad herence onto collagen and fibronectin: A comparison between jet impingement and flow chamber techniques. Materials Science and Engineering: C, 26, 260–266. DOI: 10.1016/j.msec.2005.10.051. http://dx.doi.org/10.1016/j.msec.2005.10.051CrossrefGoogle Scholar

  • [5] Bouafsoun, A., Ponsonnet, L., Kerkeni, A., Jaffrézic, N., & Othmane, A. (2007). Comparative wettability study of polystyrene functionalized with different proteins. Materials Science and Engineering: C, 27, 709–715. DOI: 10.1016/j.msec.2006.06.038. http://dx.doi.org/10.1016/j.msec.2006.06.038CrossrefWeb of ScienceGoogle Scholar

  • [6] Boura, C., Menu, P., Payan, E., Picart, C., Voegel, J. C., Muller, S., & Stoltz, J. F. (2003). Endothelial cells grown on thin polyelectrolyte multilayered films: an evaluation of a new versatile surface modification. Biomaterials, 24, 3521–3530. DOI: 10.1016/s0142-9612(03)00214-x. http://dx.doi.org/10.1016/S0142-9612(03)00214-XCrossrefGoogle Scholar

  • [7] Boura, C., Muller, S., Vautier, D., Dumas, D., Schaaf, P., Voegel, J. C., Stoltz, J. F., & Menu, P. (2005). Endothelial cell-interactions with polyelectrolyte multilayer films. Biomaterials, 26, 4568–4575. DOI: 10.1016/j.biomaterials.2004.11.036. http://dx.doi.org/10.1016/j.biomaterials.2004.11.036CrossrefGoogle Scholar

  • [8] Bowlin, G. L., & Rittgers, S. E. (1997). Electrostatic endothelial cell seeding of small diameter (<6 mm) vascular prostheses: Feasibility testing. Cell Transplantation, 6, 623–629. DOI: 10.1016/s0963-6897(97)00096-1. http://dx.doi.org/10.1016/S0963-6897(97)00096-1CrossrefGoogle Scholar

  • [9] Chluba, J., Voegel, J. C., Decher, G., Erbacher, P., Schaaf, P., & Ogier, J. (2001). Peptide hormone covalently bound to polyelectrolytes and embedded into multilayer architectures conserving full biological activity. Biomacromolecules, 2, 800–805. DOI: 10.1021/bm015529i. http://dx.doi.org/10.1021/bm015529iCrossrefGoogle Scholar

  • [10] Gammoudi, I., Tarbague, H., Othmane, A., Moynet, D., Rebi`ere, D., Kalfat, R., & Dejous, C. (2010). Love-wave bacteria-based sensor for the detection of heavy metal toxicity in liquid medium. Biosensors and Bioelectronics, 26, 1723–1726. DOI: 10.1016/j.bios.2010.07.118. http://dx.doi.org/10.1016/j.bios.2010.07.118CrossrefGoogle Scholar

  • [11] Haddad, S., Derkaoui, S. M., Avramoglou, T., Ait, E., Othmane, A., & Mora, L. (2011a). Electrochemical impedance spectroscopy as a highly sensitive tool for a dynamic interaction study between heparin and antithrombin: A novel antithrombin sensor. Talanta, 85, 927–935. DOI: 10.1016/j.talanta.2011.04.079. http://dx.doi.org/10.1016/j.talanta.2011.04.079Web of ScienceCrossrefGoogle Scholar

  • [12] Haddad, S., Zanina, N., Othmane, A., & Mora, L. (2011b). Polyurethane films modified by antithrombin-heparin complex to enhance endothelialization: An original impedimetric analysis. Electrochimica Acta, 56, 7303–7311. DOI: 10.1016/j.electacta.2011.06.048. http://dx.doi.org/10.1016/j.electacta.2011.06.048Web of ScienceCrossrefGoogle Scholar

  • [13] Ladam, G., Gergely, C., Senger, B., Decher, G., Voegel, J. C., Schaaf, P., & Cuisinier, F. J. G. (2000). Protein interactions with polyelectrolyte multilayers: Interactions between human serum albumin and polystyrene sulfonate/polyallylamine multilayers. Biomacromolecules, 1, 674–687. DOI: 10.1021/bm005572q. http://dx.doi.org/10.1021/bm005572qCrossrefGoogle Scholar

  • [14] Müller, M., Rieser, T., Dublin, P. L., & Lunkwitz, K. (2001). Selective interaction between proteins and the outermost surface of polyelectrolyte multilayers: influence of the polyanion type, pH and salt. Macromolecular Rapid Communications, 22, 390–395. DOI: 10.1002/1521-3927(20010301)22:6〈390:: AID-MARC390〉3.0.CO;2-B. http://dx.doi.org/10.1002/1521-3927(20010301)22:6<390::AID-MARC390>3.0.CO;2-BCrossrefGoogle Scholar

  • [15] Nonner, W., & Eisenberg, B. (2000). Electrodiffusion in ionic channels of biological membranes. Journal of Molecular Liquids, 87, 149–162. DOI: 10.1016/s0167-7322(00)00118-5. http://dx.doi.org/10.1016/S0167-7322(00)00118-5CrossrefGoogle Scholar

  • [16] Picart, C., Lavalle, P., Hubert, P., Cuisinier, F. J. G., Decher, G., Schaaf, P., & Voegel, J. C. (2001). Buildup mechanism for poly(L-lysine)/hyaluronic acid films onto a solid surface. Langmuir, 17, 7414–7424. DOI: 10.1021/la010848g. http://dx.doi.org/10.1021/la010848gCrossrefGoogle Scholar

  • [17] Picart, C., Senge, B., Sengupta, K., Dubreuil, F., & Fery, A. (2007). Measuring mechanical properties of polyelectrolyte multilayer thin films: Novel methods based on AFM and optical techniques. Colloids and surfaces A: Physicochemical and Engineering Aspects, 303, 30–36. DOI: 10.1016/j.colsurfa.2007.02.015. http://dx.doi.org/10.1016/j.colsurfa.2007.02.015CrossrefWeb of ScienceGoogle Scholar

  • [18] Ponsonnet, L., Comte, V., Othmane, A., Lagneau, C., Charbonnier, M., Lissac, M., & Jaffrezic, N. (2002). Effect of surface topography and chemistry on adhesion, orientation and growth of fibroblasts on nickel-titanium substrates. Materials Science and Engineering: C, 21, 157–165. DOI: 10.1016/s0928-4931(02)00097-8. http://dx.doi.org/10.1016/S0928-4931(02)00097-8CrossrefGoogle Scholar

  • [19] Ponsonnet, L., Reybier, K., Jaffrezic, N., Comte, V., Lagneau, C., Lissac, M., & Martelet, C. (2003). Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behavior. Materials Science and Engineering: C, 23, 551–560. DOI: 10.1016/s0928-4931(03)00033-x. http://dx.doi.org/10.1016/S0928-4931(03)00033-XCrossrefGoogle Scholar

  • [20] Schultz, P., Vautier, D., Richert, L., Jessel, N., Haikel, Y., Schaaf, P., Voegel, J. C., Ogier, J., & Debry, C. (2005). Polyelectrolyte multilayers functionalized by a synthetic analogue of an anti-inflammatory peptide, α-MSH, for coating a tracheal prosthesis. Biomaterials, 26, 2621–2630. DOI: 10.1016/j.biomaterials.2004.06.049. http://dx.doi.org/10.1016/j.biomaterials.2004.06.049CrossrefGoogle Scholar

  • [21] Seifalian, A. M., Tiwari, A., Hamilton, G., & Salacinski, H. J. (2002). Improving the clinical patency of prosthetic vascular and coronary bypass grafts: the role of seeding and tissue engineering. Artificial Organs, 26, 307–320. DOI: 10.1046/j.1525-1594.2002.06841.x. http://dx.doi.org/10.1046/j.1525-1594.2002.06841.xCrossrefGoogle Scholar

  • [22] Şenel, M., Coşkun, A., Fatih Abasıyanık, M., & Bozkurt, A. (2010). Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network. Chemical Papers, 64, 1–7. DOI: 10.2478/s11696-009-0103-x. http://dx.doi.org/10.2478/s11696-009-0103-xCrossrefWeb of ScienceGoogle Scholar

  • [23] Tryoen-Tóth, P., Vautier, D., Haikel, Y., Voegel, J. C., Shaaf, P., Chluba, J., & Ogier, J. (2002). Viability, adhesion, and bone phenotype of osteoblast-like cells on polyelectrolyte multilayer films. Journal of Biomedical Materials Research, 60, 657–667. DOI: 10.1002/jbm.10110. http://dx.doi.org/10.1002/jbm.10110CrossrefGoogle Scholar

  • [24] Turner, M. R. (1992). Electrical resistances of cultured bovine arterial endothelium in solutions of various resistivities. Experimental Physiology, 77, 741–748. CrossrefGoogle Scholar

  • [25] van Loosdrecht, M. C., Lyklema, J., Norde, W., & Zehnder, A. J. (1990). Influence of interfaces on microbial activity. Microbiology and Molecular Biology Reviews, 54, 75–87. Google Scholar

  • [26] van Oss, C. J. (1995). Interfacial forces in aqueous media. New York, NY, USA: Marcel Dekker. Google Scholar

  • [27] van Oss, C. J. (1997). Hydrophobicity and hydrophilicity of biosurfaces. Current Opinion in Colloid & Interface Science, 2, 503–512. DOI: 10.1016/s1359-0294(97)80099-4. http://dx.doi.org/10.1016/S1359-0294(97)80099-4CrossrefGoogle Scholar

  • [28] van Oss, C. J., Chaudhury, M. K., & Good, R. J. (1989a). The mechanism of phase separation of polymers in organic media-apolar and polar systems. Separation Sciences and Technology, 24, 15–30. DOI: 10.1080/01496398908049748. http://dx.doi.org/10.1080/01496398908049748CrossrefGoogle Scholar

  • [29] van Oss, C. J., Ju, L., Chaudhury, M. K., & Good, R. J. (1989b). Estimation of the polar parameters of the surface tension of liquids by contact angle measurements on gels. Journal of Colloid and Interface Science, 128, 313–319. DOI: 10.1016/0021-9797(89)90345-7. http://dx.doi.org/10.1016/0021-9797(89)90345-7CrossrefGoogle Scholar

  • [30] Vautier, D., Karsten, V., Egles, C., Chluba, J., Schaaf, P., Voegel, J. C., & Ogier, J. (2002). Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Journal of Biomaterials Science, Polymer Edition, 13, 712–731. DOI: 10.1163/156856202320269175. http://dx.doi.org/10.1163/156856202320269175CrossrefGoogle Scholar

  • [31] Velzenberger, E., El Kirat, K., Legeay, G., Nagel, M. D., & Pezron, I. (2009). Characterization of biomaterials polar interactions in physiological conditions using liquid-liquid contact angle measurements: Relation to fibronectin adsorption. Colloids and Surfaces B: Biointerfaces, 68, 238–244. DOI: 10.1016/j.colsurfb.2008.10.022. http://dx.doi.org/10.1016/j.colsurfb.2008.10.022CrossrefWeb of ScienceGoogle Scholar

  • [32] Vodouhê, C., Schmittbuhl, M., Boulmedais, F., Bagnard, D., Vautier, D., Schaaf, P., Egles, C., Voegel, J. C., & Ogier, J. (2005). Effect of fonctionnalization of multilayered polyelectrolyte films on motoneuron growth. Biomaterials, 26, 545–554. DOI: 10.1016/j.biomaterials.2004.02.057. http://dx.doi.org/10.1016/j.biomaterials.2004.02.057CrossrefGoogle Scholar

  • [33] Vuori, K. (1998). Integrin signaling: Tyrosine phosphorylation events in focal adhesions. Journal of Membrane Biology, 165, 191–199. DOI: 10.1007/s002329900433. http://dx.doi.org/10.1007/s002329900433CrossrefGoogle Scholar

  • [34] Waldeck, H., & Kao, W. J. (2008). Extended interaction of β1 integrin subunit-deficient cells (GD25) with surfaces modified with fibronectin-derived peptides: Culture optimization, adhesion and cytokine panel studies. Acta Biomaterialia, 4, 1172–1186. DOI: 10.1016/j.actbio.2008.03.020. http://dx.doi.org/10.1016/j.actbio.2008.03.020Web of ScienceGoogle Scholar

  • [35] Wang, C. H., Luo, C. W., Huang, C. F., Huang, M. S., Ou, K. L., & Yu, C. H. (2011). Biocompatibility of metal carbides on Fe-Al-Mn-based alloys. Journal of Alloys and Compounds, 509, 691–696. DOI: 10.1016/j.jallcom.2010.06.071. http://dx.doi.org/10.1016/j.jallcom.2010.06.071CrossrefGoogle Scholar

  • [36] Xi, F., Gao, J., Wang, J., & Wang, Z. (2011). Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer. Journal of Electroanalytical Chemistry, 656, 252–257. DOI: 10.1016/j.jelechem.2010.10.025. http://dx.doi.org/10.1016/j.jelechem.2010.10.025CrossrefGoogle Scholar

  • [37] Zhang, P., Qian, J., Yang, Y., An, Q., Liu, X., & Gui, Z. (2008). Polyelectrolyte layer-by-layer self-assembly enhanced by electric field and their multilayer membranes for separating isopropanol-water mixtures. Journal of Membrane Science, 320, 73–77. DOI: 10.1016/j.memsci.2008.03.055. http://dx.doi.org/10.1016/j.memsci.2008.03.055CrossrefWeb of ScienceGoogle Scholar

  • [38] Zhu, Y., & Sun, Y. (2004). The influence of polyelectrolyte charges of polyurethane membrane surface on the growth of human endothelial cells. Colloids and Surfaces B: Biointerfaces, 36, 49–55. DOI: 10.1016/j.colsurfb.2004.04.008. http://dx.doi.org/10.1016/j.colsurfb.2004.04.008CrossrefGoogle Scholar

About the article

Published Online: 2012-04-05

Published in Print: 2012-05-01

Citation Information: Chemical Papers, Volume 66, Issue 5, Pages 532–542, ISSN (Online) 1336-9075, DOI: https://doi.org/10.2478/s11696-012-0141-7.

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