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Volume 66, Issue 9

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Identification and determination of relatedness of lactobacilli using different DNA amplification methods

Kristýna Turková
  • Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, CZ-612 00, Brno, Czech Republic
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/ Bohuslav Rittich
  • Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, CZ-612 00, Brno, Czech Republic
  • Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, CZ-602 00, Brno, Czech Republic
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/ Alena Španová
  • Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, CZ-612 00, Brno, Czech Republic
  • Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, CZ-602 00, Brno, Czech Republic
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Published Online: 2012-06-22 | DOI: https://doi.org/10.2478/s11696-012-0206-7

Abstract

Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces (31 strains), dairy products (5 strains) and silage (1 strain). Twenty-seven strains isolated from infant faeces were identified as Lactobacillus rhamnosus (9), Lactobacillus gasseri (6), Lactobacillus paracasei (4), Lactobacillus fermentum (4), Lactobacillus salivarius (2), Lactobacillus plantarum (1), and Lactobacillus helveticus (1) using 10 species-specific polymerase chain reactions (PCRs), multiplex PCR for the Lactobacillus casei group, and sequencing of 16S rDNA. Four strains were not identified. Six strains isolated from dairy products and silage were identified as Lactobacillus rhamnosus. A repetitive extragenic palindromic polymerase chain reaction (rep-PCR) with primer (GTG)5 and a randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) with primer M13 were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.

Keywords: Lactobacillus; breastfed infants; dairy products; identification; PCR; fingerprinting

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About the article

Published Online: 2012-06-22

Published in Print: 2012-09-01


Citation Information: Chemical Papers, Volume 66, Issue 9, Pages 842–851, ISSN (Online) 1336-9075, DOI: https://doi.org/10.2478/s11696-012-0206-7.

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© 2012 Institute of Chemistry, Slovak Academy of Sciences.

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