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Current Issues in Pharmacy and Medical Sciences

Formerly Annales UMCS Sectio DDD Pharmacia

CiteScore 2018: 0.32

SCImago Journal Rank (SJR) 2018: 0.154
Source Normalized Impact per Paper (SNIP) 2018: 0.285

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Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Urszula KosikowskaORCID iD: https://orcid.org/0000-0003-4536-1750 / Edyta ChwiejczakORCID iD: https://orcid.org/0000-0003-2287-6786 / Artur Niedzielski
  • Department of Pediatric Otolaryngology, Phoniatrics and Audiology, Medical University of Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sylwia AndrzejczukORCID iD: https://orcid.org/0000-0001-6301-6059 / Anna MalmORCID iD: https://orcid.org/0000-0003-1503-7634
Published Online: 2019-12-31 | DOI: https://doi.org/10.2478/cipms-2019-0032


Introduction. Haemophilus influenzae and Haemophilus parainfluenzae are known as human-restricted respiratory microbiota representatives. The aim of the present paper was to assay haemophili prevalence in middle ear effusion specimens in pediatric patients with otitis media with effusion (OME).

Methods. A total of 86 ear effusion specimens (from the left and right ear independently) were collected from 43 pediatric patients with OME. For comparison, 58 nasopharyngeal specimens were taken from 58 pediatric patients prone to recurrent respiratory tract infections (RRTI). Isolation and identification of haemophili biotypes and antimicrobial susceptibility was accomplished by standard microbiological methods. The cell surface hydrophobicity (CSH) of isolates was assayed by the method of aggregation in ammonium sulfate (SAT).

Results. Haemophili were isolated in 25.6% (11/43) of all OME patients: in 5/43 (11.6%)– H. influenzae (biotypes II, III), in 5/43 (11.6%) – H. parainfluenzae, in 1/43 (2.3%) – both species were found. Haemophili-positive nasopharyngeal specimen was found in 27/58 (46.6%) RRTI patients: in 19/58 (32.8%) – H. influenzae, in 8/58 (13.8%) – H. parainfluenzae. About 90% of all haemophili isolates were characterised by extreme to strong CSH. Antimicrobial resistance occurred mainly among H. parainfluenzae (80%) and to a much lower percentage among H. influenzae (33.3%) isolates. The obtained data suggest that both H. influenzae and H. parainfluenzae can be involved in pathology of OME in pediatric patients. The high cell surface hydrophobicity can affect on the haemophili prevalence and ear colonization, and induces predisposition to the presence of these bacteria as a biofilm that serves as a virulence factor with great importance for the survival of these opportunistic bacteria and their persistence in the ear environment.

Keywords: Haemophilus influenzae; Haemophilus parainfluenzae; pediatric patients; otitis media with effusion; bacterial cell surface hydrophobicity


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About the article

Received: 2019-05-06

Accepted: 2019-09-30

Published Online: 2019-12-31

Published in Print: 2019-12-01

Citation Information: Current Issues in Pharmacy and Medical Sciences, Volume 32, Issue 4, Pages 183–188, ISSN (Online) 2300-6676, DOI: https://doi.org/10.2478/cipms-2019-0032.

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© 2019 Urszula Kosikowska et al., published by Sciendo. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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