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Cellular and Molecular Biology Letters

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Volume 11, Issue 2

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The assessment of DNA from marine organisms via a modified salting-out protocol

Giovanni Ferrara / Barbara Murgia / Anna Parodi / Laura Valisano
  • Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy
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/ Carlo Cerrano
  • Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy
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/ Giulio Palmisano / Giorgio Bavestrello / Michele Sara
  • Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy
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Published Online: 2006-06-01 | DOI: https://doi.org/10.2478/s11658-006-0013-7

Abstract

We developed a rapid, practical and non-toxic salting-out method for the extraction of DNA from marine organisms, and tested it on two representative species of Porifera and Cnidaria, both living in association with symbiotic zooxanthellae. We tested the efficiency of the protocol by comparing the output of the method for fresh tissue, frozen tissue and tissue stored in ethanol. It proved to be effective for extracting DNA in the case of the methods of preservation considered here, and for obtaining quantities of DNA comparable to those obtained via the traditional approach. The DNA from both species was of good quality. The DNA obtained was amplified by PCR using specific primers for the large ribosomal subunit, allowing the identification of the presence of both the host and symbiont genomes.

Keywords: Symbiodinium; Porifera; Cnidaria; DNA; PCR

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About the article

Published Online: 2006-06-01

Published in Print: 2006-06-01


Citation Information: Cellular and Molecular Biology Letters, Volume 11, Issue 2, Pages 155–160, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-006-0013-7.

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© 2006 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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