Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Cellular and Molecular Biology Letters

More options …
Volume 11, Issue 2


The assessment of DNA from marine organisms via a modified salting-out protocol

Giovanni Ferrara / Barbara Murgia / Anna Parodi / Laura Valisano
  • Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Carlo Cerrano
  • Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Giulio Palmisano / Giorgio Bavestrello / Michele Sara
  • Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2006-06-01 | DOI: https://doi.org/10.2478/s11658-006-0013-7


We developed a rapid, practical and non-toxic salting-out method for the extraction of DNA from marine organisms, and tested it on two representative species of Porifera and Cnidaria, both living in association with symbiotic zooxanthellae. We tested the efficiency of the protocol by comparing the output of the method for fresh tissue, frozen tissue and tissue stored in ethanol. It proved to be effective for extracting DNA in the case of the methods of preservation considered here, and for obtaining quantities of DNA comparable to those obtained via the traditional approach. The DNA from both species was of good quality. The DNA obtained was amplified by PCR using specific primers for the large ribosomal subunit, allowing the identification of the presence of both the host and symbiont genomes.

Keywords: Symbiodinium; Porifera; Cnidaria; DNA; PCR

  • [1] Glynn, P.W. Coral reef bleaching in the 1980s and possible connections with global warming. Trends Ecol. Evol. 6 (1991) 175–179. http://dx.doi.org/10.1016/0169-5347(91)90208-FCrossrefGoogle Scholar

  • [2] Hayes, M.L., Bonaventura, J., Mitchell, T.P., Prosper, J.M., Shinn, E.A., Van Dolah, F. and Barber, R.T. How are climate and marine biological outbreaks functionally linked? Hydrobiologia 460 (2001) 213–220. http://dx.doi.org/10.1023/A:1013121503937CrossrefGoogle Scholar

  • [3] Muller-Parker, G. and D’Elia, C.F. Interactions between corals and their symbiotic algae. In: Life and Death of Coral Reefs (Birkeland, C. Ed.) Chapman & Hall, New York., 1997, 96–113. Google Scholar

  • [4] Wakefield, T.S., Farmer, M.A. and Kempf, S.C. Revised description of the fine structure of in situ “zooxanthellae” genus Symbiodinium. Biol. Bull. 199 (2000) 76–84. Google Scholar

  • [5] Goulet, T.L. and Coffroth, M.A. Stability of an octocoral-algal symbiosis over time and space. Mar. Ecol. Prog. Ser. 250 (2003) 117–124. Google Scholar

  • [6] Rowan, R., Knowlton, N., Baker, A.C. and Jara, J. Landscape ecology of algal symbiont communities explains variation in episodes of coral bleaching. Nature 388 (1997) 265–269. http://dx.doi.org/10.1038/40843CrossrefGoogle Scholar

  • [7] Cowen, R. The role of algal Symbiosis in reefs through time. Palaios 3 (1998) 221–227. CrossrefGoogle Scholar

  • [8] Hoegh-Guldberg, O. Climate change, coral bleaching and the future of the world’s coral reefs. Mar. Freshw. Res. 50 (1999) 839–866. http://dx.doi.org/10.1071/MF99078CrossrefGoogle Scholar

  • [9] Muscatine, L. and Porter, J.W. Reef corals — mutualistic symbioses adapted to nutrient-poor environments. BioScience 27 (1997) 454–460. http://dx.doi.org/10.2307/1297526CrossrefGoogle Scholar

  • [10] Junghans, H. and Metzlaff, M. A simple and rapid method for preparation of total plant DNA. Biotechnique 8 (1990) 176. Google Scholar

  • [11] Coffroth, M.A., Lasker, H.R., Diamond, M.E., Bruenn, J.A. and Bermingham, E. DNA fingerprints of a gorgonian coral: a method for detecting clonal structure in a vegetative species. Mar. Biol. 114 (1992) 317–325. http://dx.doi.org/10.1007/BF00349534CrossrefGoogle Scholar

  • [12] Sinha, R.P, Dautz, M. and Hader, D.P. A simple and Efficient Method for the Quantitative Analysis of Thymine Cyanobacteria, Phytoplancton and Macroalgae. Acta Protozool. 40 (2001) 187–195. Google Scholar

  • [13] Chen, C.A. and Yu, J.K. Universal primers for amplification of mithochondrial small subunit ribosomal RNA-encoding gene in scleractinian corals. Mar. Biotechnol. 2 (2000) 146–153. http://dx.doi.org/10.1007/s101260000040CrossrefGoogle Scholar

  • [14] LaJeunesse, T.C. Investigating the biodiversity, ecology and phylogeny of endosymbiotic dinoflagellates in the genus Symbiodinium using ITS region. In: Search of a “specie” level marker. J. Phycol. 37 (2001) 866–880. http://dx.doi.org/10.1046/j.1529-8817.2001.01031.xCrossrefGoogle Scholar

  • [15] Santos, S.R., Gutierrez-Riodriguez, C., Lasker, H.R. and Coffroth, M.A. Symbiodinium sp. Association in the gorgonian Pseudopterogorgia elisabethae in the Bahamas: high level of genetic variability and population structure in symbiotic dinoflagellates. Mar. Biol. 143 (2003) 111–120. http://dx.doi.org/10.1007/s00227-003-1065-0CrossrefGoogle Scholar

  • [16] Crabbe, M.J. A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinians corals. J. Biochem. Biophys. Methods 57 (2003) 171–176. http://dx.doi.org/10.1016/S0165-022X(03)00051-4CrossrefGoogle Scholar

  • [17] Miller, S.A., Dykes, D.D. and Polesky, H.F. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res. 16 (1988), 1215. Google Scholar

  • [18] Rawlings, L.H. and Donellan, S.C. Phylogeographic analysis of the green phyton, Morelia viridis, reveals criptic diversity. Mol. Phylogen. Evol. 27 (2003) 36–44. http://dx.doi.org/10.1016/S1055-7903(02)00396-2CrossrefGoogle Scholar

  • [19] Underwood, A.J. Techniques of analysis of variance in experimental marine biology and ecology. Annu. Rev. Ocean. Mar. Biol. 19 (1981) 513–605. Google Scholar

About the article

Published Online: 2006-06-01

Published in Print: 2006-06-01

Citation Information: Cellular and Molecular Biology Letters, Volume 11, Issue 2, Pages 155–160, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-006-0013-7.

Export Citation

© 2006 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Anchalee Chankong, Narinratana Kongjandtre, Wansuk Senanan, and Vipoosit Manthachitra
Regional Studies in Marine Science, 2019
Rodrigo Carballo-Bolaños, Vianney Denis, Ya-Yi Huang, Shashank Keshavmurthy, Chaolun Allen Chen, and Senjie Lin
PLOS ONE, 2019, Volume 14, Number 6, Page e0218801
George Seghal Kiran, Lipton Anuj Nishanth, Sethu Priyadharshini, Kumar Anitha, and Joseph Selvin
BMC Biotechnology, 2014, Volume 14, Number 1
Jaclyn A. Mueller, Alexander I. Culley, Grieg F. Steward, and K. E. Wommack
Applied and Environmental Microbiology, 2014, Volume 80, Number 13, Page 3930
Shashank Keshavmurthy, Kuo-Hsun Tang, Chia-Min Hsu, Chai-Hsia Gan, Chao-Yang Kuo, Keryea Soong, Hong-Nong Chou, and Chaolun Allen Chen
PeerJ, 2017, Volume 5, Page e2871
Silvia Fontana, Shashank Keshavmurthy, Hernyi Justin Hsieh, Vianney Denis, Chao-Yang Kuo, Chia-Ming Hsu, Julia K. L. Leung, Wan-Sen Tsai, Carden C. Wallace, Chaolun Allen Chen, and Christopher Fulton
PLoS ONE, 2012, Volume 7, Number 11, Page e50130
Chuan-Ho Tang, Ping-Chang Ku, Ching-Yu Lin, Te-Hao Chen, Kuo-Hsin Lee, Shu-Hui Lee, and Wei-Hsien Wang
Marine Biotechnology, 2015, Volume 17, Number 5, Page 633
Joana Rocha, Francisco J. R. C. Coelho, Luísa Peixe, Newton C. M. Gomes, and Ricardo Calado
Scientific Reports, 2015, Volume 4, Number 1
Shashank Keshavmurthy, Pei-Jie Meng, Jih-Terng Wang, Chao-Yang Kuo, Sung-Yin Yang, Chia-Min Hsu, Chai-Hsia Gan, Chang-Feng Dai, and Chaolun Allen Chen
PeerJ, 2014, Volume 2, Page e327
George S. Kiran, Balu Sabarathnam, Nooruddin Thajuddin, and Joseph Selvin
Journal of Surfactants and Detergents, 2014, Volume 17, Number 3, Page 531
Molecular Ecology, 2012, Volume 21, Number 17, Page 4393
Donna-Mareè Cawthorn, Harris Andrew Steinman, and R. Corli Witthuhn
Food Control, 2011, Volume 22, Number 2, Page 231
G. Seghal Kiran, T. Anto Thomas, and Joseph Selvin
Colloids and Surfaces B: Biointerfaces, 2010, Volume 78, Number 1, Page 8

Comments (0)

Please log in or register to comment.
Log in