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Cellular and Molecular Biology Letters

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Volume 11, Issue 2

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Identification of microsatellite markers in the rye genome

Hanna Bolibok
  • Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, 02-787, Warszawa, Poland
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/ Monika Rakoczy-Trojanowska
  • Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, 02-787, Warszawa, Poland
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/ Małgorzata Wyrzykowska
  • Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, 02-787, Warszawa, Poland
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/ Magdalena Radecka / Wacław Orczyk
Published Online: 2006-06-01 | DOI: https://doi.org/10.2478/s11658-006-0023-5

Abstract

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.

Keywords: Rye; Genomic library; SSR; Microsatellite; Wheat-rye addition lines

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About the article

Published Online: 2006-06-01

Published in Print: 2006-06-01


Citation Information: Cellular and Molecular Biology Letters, Volume 11, Issue 2, Pages 291–298, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-006-0023-5.

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© 2006 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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