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BY-NC-ND 3.0 license Open Access Published by De Gruyter September 1, 2006

Genotyping of recombinant Pichia pastoris strains

  • José Viader-Salvadó EMAIL logo , Eddy Cab-Barrera , Luis Galán-Wong and Martha Guerrero-Olazarán

Abstract

A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.

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Published Online: 2006-9-1
Published in Print: 2006-9-1

© 2006 University of Wrocław, Poland

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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