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Cellular and Molecular Biology Letters

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Volume 12, Issue 3

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The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Ewa Macierzyńska / Agnieszka Grzelak / Grzegorz Bartosz
  • Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland
  • Department of Biochemistry and Cell Biology, University of Rzeszów, Cegielniana 12, 35-595, Rzeszów, Poland
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Published Online: 2007-03-15 | DOI: https://doi.org/10.2478/s11658-007-0017-y

Abstract

We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.

Keywords: Yeast; Saccharomyces cerevisiae; Reactive oxygen species; Superoxide dismutase; Catalase; Glutathione

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About the article

Published Online: 2007-03-15

Published in Print: 2007-09-01


Citation Information: Cellular and Molecular Biology Letters, Volume 12, Issue 3, Pages 448–456, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-007-0017-y.

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© 2007 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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