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Cellular and Molecular Biology Letters

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Volume 13, Issue 1 (Mar 2008)

Syntaxin 8 has two functionally distinct di-leucine-based motifs

Kazuo Kasai
  • Department of Cell Physiology, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan
  • Toyama Chemical Co., Ltd., Shinjuku, Tokyo, 160-0023, Japan
  • Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan
  • Email:
/ Kei Suga
  • Department of Cell Physiology, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan
  • Email:
/ Tetsuro Izumi
  • Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan
  • Email:
/ Kimio Akagawa
  • Department of Cell Physiology, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan
  • Email:
Published Online: 2007-10-29 | DOI: https://doi.org/10.2478/s11658-007-0043-9


Syntaxin 8 has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These have been shown to function as the machinery for the homotypic fusion of late endosomes. Recently, we showed that syntaxins 7 and 8 cycle through the plasma membrane, and that the di-leucine-based motifs in the cytoplasmic domain of syntaxins 7 and 8 respectively function in their endocytic and exocytic processes. However, we could not elucidate the mechanism by which syntaxin 8 cycles through the plasma membrane. In this study, we constructed several different syntaxin 8 molecules by mutating putative di-leucine-based motifs, and analyzed their intracellular localization and trafficking. We found a di-leucine-based motif in the cytoplasmic domain of syntaxin 8. It is similar to that of syntaxin 7, and functions in its endocytosis. These results suggest that in the cytoplasmic domain, syntaxin 8 has two functionally distinct di-leucine-based motifs that act independently in its endocytic and exocytic processes. This is the first report on two di-leucine-based motifs in the same molecule acting independently in distinct transport pathways.

Keywords: Syntaxin; Di-leucine-based motif; Endocytosis; Exocytosis

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About the article

Published Online: 2007-10-29

Published in Print: 2008-03-01

Citation Information: Cellular and Molecular Biology Letters, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-007-0043-9. Export Citation

© 2007 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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