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Cellular and Molecular Biology Letters

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Volume 13, Issue 2 (Jun 2008)

The cytoplasmic domain of chondrolectin interacts with the β-subunit of rab geranylgeranyl transferase

An Claessens
  • Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium
  • Email:
/ Christine Weyn
  • Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium
  • Email:
/ Joseph Merregaert
  • Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium
  • Email:
Published Online: 2008-04-09 | DOI: https://doi.org/10.2478/s11658-007-0052-8


Mouse chondrolectin (chodl) was isolated out of the tail tip of four-day old 129/SvJ mice as a by-product of a PCR-based subtractive cDNA library screening. The gene is predominantly expressed in adult skeletal muscle, heart, testes and lungs and in embryonic stadia. Chodl is the mouse homologue of human chondrolectin (CHODL), a gene that encodes for a type Ia transmembrane protein and that is expressed in human testis, prostate, heart and skeletal muscle tissue. CHODL-splice variants (CHODL f, CHODL fΔE, CHODL ΔE) are detected in human leukocytes. The proteins of the chondrolectin family belong to the family of C-type lectins. As the members of this protein family are important for a wide array of biological processes, the function of chodl was investigated by searching for its protein interaction partners. The β-subunit of Rab geranylgeranyl transferase (Rabggtb) was isolated 8 times after a complete Sos recruitment system (SRS) screen with the cytoplasmic domain of chodl. The interaction was confirmed with in vitro transcription/translation and co-immunoprecipitation (co-IP) experiments.

Keywords: C-type lectin; Chondrolectin; SRS

  • [1] Weng, L., Hübner, R., Claessens, A., Smits, P., Wauters, J., Tylzanowski, P., Van Marck, E. and Merregaert, J. Isolation and characterization of a novel C-type lectin, Chondrolectin (Chodl), which is predominantly expressed in muscle cells. Gene 308 (2003) 21–29. http://dx.doi.org/10.1016/S0378-1119(03)00425-6 [Crossref]

  • [2] Weng, L., Smits, P., Wauters, J. and Merregaert, J. Molecular cloning and characterization of human Chondrolectin, a novel type I transmembrane protein homologous to C-type lectins. Genomics 80 (2002) 62–70. http://dx.doi.org/10.1006/geno.2002.6806 [Crossref]

  • [3] Claessens, A., Van de Vijver, K., Van Bockstaele, D.R., Wauters, J., Berneman, Z.W., Van Marck, E. and Merregaert, J. Expression and localization of CHODLdeltaE/CHODLfdeltaE, the soluble isoform of chondrolectin. Cell Biol. Int. 31 (2007) 1323–1330. http://dx.doi.org/10.1016/j.cellbi.2007.05.014 [Crossref] [Web of Science]

  • [4] Weng, L., Van Bockstaele, D.R., Wauters, J., Van Marck, E., Plum, J., Berneman, Z.N. and Merregaert, J. A novel alternative spliced Chondrolectin isoform lacking the transmembrane domain is expressed during T cell maturation. J. Biol. Chem. 278 (2003) 19164–19170. http://dx.doi.org/10.1074/jbc.M300653200 [Crossref]

  • [5] Drickamer, K. Ca2+-dependent carbohydrate-recognition domains in animal proteins. Curr. Opin. Struct. Biol. 3 (1993) 393–400. http://dx.doi.org/10.1016/S0959-440X(05)80112-5

  • [6] Drickamer, K. and Taylor, M.E. Biology of animal lectins. Annu. Rev. Cell. Biol. 9 (1993) 237–264. http://dx.doi.org/10.1146/annurev.cb.09.110193.001321 [Crossref]

  • [7] Zelensky, A.N. and Gready, J.E. The C-type lectin-like domain superfamily. FEBS Lett. 272 (2005) 6179–6217.

  • [8] Kresse, H. and Schönherr, E. Proteoglycans of the extracellular matrix and growth control. J. Cell. Physiol. J. Cell. Physiol. (2001) 266–274. [Crossref]

  • [9] Ellgaard, L. and Helenius, A. Quality control in the endoplasmic reticulum. Nat. Rev. 4 (2003) 181–191. http://dx.doi.org/10.1038/nrm1052 [Crossref]

  • [10] Aronheim, A., Zandi, E., Hennemann, H., Elledge, S.J. and Karin, M. Isolation of an AP-1 repressor by a novel method for detecting proteinprotein interactions. Mol. Cell. Biol. 17 (1997) 3094–3102.

  • [11] Huang, W., Wang, S., Lozano, G. and de Crombrugghe, B. cDNA library screening using the Sos recruitment system. Biotechniques 30 (2001) 94–100.

  • [12] Beil, B., Screaton, G. and Stamm, S. Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing. DNA Cell Biol. 16 (1997) 679–690.

  • [13] Chinpaisal, C., Lee, C. and Wei, L. Studies of the mouse Rab geranylgeranyl transferase β-subunit: gene structure, expression and regulation. Gene 184 (1997) 237–243. http://dx.doi.org/10.1016/S0378-1119(96)00605-1 [Crossref]

  • [14] Armstrong, S.A., Seabra, M.C., Sudhof, T.C., Goldstein, J.L. and Brown, M.S. cDNA cloning and expression of the α and β subunits of Rat Rab geranylgeranyl transferase. J. Biol. Chem. 268 (1993) 12221–12229.

  • [15] Khosravi-Far, R., Clark, G.J., Abe, K., Cox, A.D., McLain, T., Lutz, R.J., Sinensky, M. and Der, C.J. Ras (CXXX) and Rab (CC/CXC) prenylation signal sequences are unique and functionally distinct. J. Biol. Chem. 267 (1992) 24363–24368.

  • [16] Leung, K.F., Baron, R., Ali, B.R., Magree, A.I. and Seabra, M.C. Rab GTPases containing a CAAX motif are processed post-geranylgeranylation by proteolysis and methylation. J. Biol. Chem. 282 (2007) 1487–1497. http://dx.doi.org/10.1074/jbc.M605557200 [Crossref] [Web of Science]

About the article

Published Online: 2008-04-09

Published in Print: 2008-06-01

Citation Information: Cellular and Molecular Biology Letters, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-007-0052-8. Export Citation

© 2007 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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