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Cellular and Molecular Biology Letters

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Volume 14, Issue 2 (Jun 2009)

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100

Mirosława Panasiewicz
  • Departments of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warszawa, Poland
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/ Hanna Domek
  • Departments of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warszawa, Poland
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/ Grażyna Hoser
  • Department of Clinical Cytology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warszawa, Poland
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/ Natalia Fedoryszak
  • Departments of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warszawa, Poland
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/ Maciej Kawalec
  • Department of Clinical Cytology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warszawa, Poland
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/ Tadeusz Pacuszka
  • Departments of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warszawa, Poland
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Published Online: 2009-03-13 | DOI: https://doi.org/10.2478/s11658-008-0043-4

Abstract

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

Keywords: Ceramide; Gangliosides; GM1; Membrane domains; Myristic acid; Sonication

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About the article

Published Online: 2009-03-13

Published in Print: 2009-06-01


Citation Information: Cellular and Molecular Biology Letters, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-008-0043-4.

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© 2008 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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