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Volume 15, Issue 2 (Jun 2010)

Flavonoid transport across RBE4 cells: A blood-brain barrier model

Ana Faria
  • Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal
  • Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal
  • Email:
/ Diogo Pestana
  • Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal
  • Email:
/ Diana Teixeira
  • Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal
  • Email:
/ Joana Azevedo
  • Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal
  • Email:
/ Victor Freitas
  • Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal
  • Email:
/ Nuno Mateus
  • Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal
  • Email:
/ Conceição Calhau
  • Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal
  • Email:
Published Online: 2010-03-25 | DOI: https://doi.org/10.2478/s11658-010-0006-4


There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer’s and Parkinson’s diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.

Keywords: Anthocyanin; Blood-brain barrier; Flavonol; 3-flavanol; RBE4; Transport

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About the article

Published Online: 2010-03-25

Published in Print: 2010-06-01

Citation Information: Cellular and Molecular Biology Letters, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-010-0006-4. Export Citation

© 2010 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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