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Cellular and Molecular Biology Letters

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1689-1392
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Volume 15, Issue 4

ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site

Lu Xue / Hongling Qiu / Jian Ma / Mingxiong Guo / Wenxin Li
Published Online: 2010-09-24 | DOI: https://doi.org/10.2478/s11658-010-0025-1

Abstract

ZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.

Keywords: ZNF300; Egr-1; IL-2Rβ promoter; Activation

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About the article

Published Online: 2010-09-24

Published in Print: 2010-12-01


Citation Information: Cellular and Molecular Biology Letters, Volume 15, Issue 4, Pages 530–540, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-010-0025-1.

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© 2010 University of Wrocław, Poland. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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