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Cellular and Molecular Biology Letters

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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells

1Lund University

2V. A. Almazov Federal Center for Heart

3Russian Academy of Sciences

4Université Laval

5University of Copenhagen

6IVF Kliniken Cura

© 2011 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

Citation Information: Cellular and Molecular Biology Letters. Volume 16, Issue 1, Pages 79–88, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-010-0039-8, January 2011

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The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

Keywords: Human embryonic stem cells; Feeder cells; DNA microarray

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