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Cellular and Molecular Biology Letters

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1689-1392
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Volume 16, Issue 4

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Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

Ammad Khan / Takashi Shimokawa / Staffan Strömblad / Hongquan Zhang
Published Online: 2011-09-29 | DOI: https://doi.org/10.2478/s11658-011-0028-6

Abstract

Kindlin-2 is a recently identified FERM and PH domain containing integrin interacting protein. Kindlin-2 is ubiquitously expressed in normal tissues. So far, much effort has been spent exploring the functional aspects of Kindlin-2. However, the transcriptional regulation of Kindlin-2 has not yet been investigated. In this study we identified and functionally characterized the promoter of the human Kindlin-2 gene. We show that the core promoter of Kindlin-2 is a 39 base pair long GC rich fragment located −122/-83 upstream of the Kindlin-2 transcription start site. Functional characterization of this core promoter region by both in silico as well as in vitro/in vivo analysis shows that the transcription factor SP1 plays an important role in regulation of Kindlin-2 expression.

Keywords: Kindlin-2; FERMT2; PLEKHC1; Mig-2; Transcription factor SP1; Promoter; Transcription start site; Gene regulation; Cis-acting elements; CpG island; Cell migration; Integrin; Gene expression

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About the article

Published Online: 2011-09-29

Published in Print: 2011-12-01


Citation Information: Cellular and Molecular Biology Letters, Volume 16, Issue 4, Pages 638–651, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-011-0028-6.

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© 2011 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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