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Cellular and Molecular Biology Letters

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Volume 19, Issue 1 (Mar 2014)

MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene

Qiu Chen / Lei Li / Yu Tu / Lu Zheng / Wei Liu / Xue Zuo / Yong He / Shu Zhang / Wei Zhu / Jian Cao / Feng Cui / Jun Hou
Published Online: 2014-03-26 | DOI: https://doi.org/10.2478/s11658-013-0115-y

Abstract

MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.

Keywords: MiR-34a; KLF4; Apoptosis; Liver; Irradiation; Doxorubicin

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About the article

Published Online: 2014-03-26

Published in Print: 2014-03-01


Citation Information: Cellular and Molecular Biology Letters, ISSN (Online) 1689-1392, DOI: https://doi.org/10.2478/s11658-013-0115-y.

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