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Volume 20, Issue 5


Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells

Flora De Conto / Carlo Chezzi / Alessandra Fazzi / Sergey V. Razin
  • Institute of Gene Biology, Russian Academy of Sciences and Lomonosow Moscow State University, Moscow, Russia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maria Cristina Arcangeletti / Maria Cristina Medici / Rita Gatti
  • Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Parma, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Adriana Calderaro
Published Online: 2016-03-05 | DOI: https://doi.org/10.1515/cmble-2015-0052


The dynamics of microtubule networks are known to have an impact on replication of influenza A virus in some cellular models. Here we present evidence suggesting that at late stages of LLC-MK2 cell infection by influenza A (H1N1) virus the ubiquitin-proteasome protein degradation system participates in destabilization of microtubules, and favours virus replication. Chemical inhibition of proteasome activity partially suppresses influenza A virus replication, while stimulation of proteasome activity favours influenza A virus replication. Conversely, in another cellular model, A549 cells, inhibitors and activators of proteasomes have a small effect on influenza A virus replication. These data suggest that influenza A virus might take selective advantage of proteasome functions in order to set up a favourable cytoskeletal “environment” for its replication and spread. Furthermore, the relationship between influenza virus and the host cell is likely to depend on both the cellular model and the virus strain.

Keywords: Influenza A virus; Microtubule cytoskeleton; Viral nucleoprotein; Virus-host interaction; Proteasomes; Acetylated alpha-tubulin; Microtubuleassociated protein 4; Gene expression; MG132; IU1


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About the article

Received: 2015-06-26

Accepted: 2015-11-04

Published Online: 2016-03-05

Published in Print: 2015-12-01

Citation Information: Cellular and Molecular Biology Letters, Volume 20, Issue 5, Pages 840–866, ISSN (Online) 1689-1392, DOI: https://doi.org/10.1515/cmble-2015-0052.

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