Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Chemical Product and Process Modeling

Ed. by Sotudeh-Gharebagh, Rhamat / Mostoufi, Navid / Chaouki, Jamal

CiteScore 2018: 0.88

SCImago Journal Rank (SJR) 2018: 0.211
Source Normalized Impact per Paper (SNIP) 2018: 0.368

See all formats and pricing
More options …

Influence of Phase Compositions, pH and Temperature on Bovine Serum Albumin Partitioning in Aqueous Biphasic System

Perumalsamy Muthiah / Bharat Kumar Pampana
Published Online: 2010-06-21 | DOI: https://doi.org/10.2202/1934-2659.1452

The partitioning of bovine serum albumin (BSA) was investigated in polyethylene glycol (PEG) 4000-sodium citrate system at 308K and 318K with varying pHs and tie line lengths (TLL). The systems were prepared at a pH of 5.2, 7.1 and 9.2 with different TLL compositions. The affinity of the BSA for the lower phase increases with an increase in pH, which is due to an increase of trivalent to divalent citrate ions in the two phase system. The affinity of the BSA to the upper phase increases with an increase in TLL, which is due to the increase in available free volume in the upper phase. As the temperature increases, the partition coefficient of BSA shows increment.

Keywords: aqueous biphasic system; bovine serum albumin; pH; tie line length; polyethylene glycol; sodium citrate

About the article

Published Online: 2010-06-21

Citation Information: Chemical Product and Process Modeling, Volume 5, Issue 1, ISSN (Online) 1934-2659, DOI: https://doi.org/10.2202/1934-2659.1452.

Export Citation

©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in