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DNA Barcodes

Ed. by Mitchell, Andrew

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Emerging Science

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2299-1077
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DNA barcoding of the vegetable Leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) in Bangladesh

Sabrina Amin
  • Corresponding author
  • Department of Biochemistry & Biotechnology, University of Science & Technology Chittagong, Bangladesh
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/ Sonja J. Scheffer / Matthew L. Lewis / Mostafa Kamal Pasha / Badrul Amin Bhuiya
Published Online: 2014-08-27 | DOI: https://doi.org/10.2478/dna-2014-0005

Abstract

DNA barcoding revealed the presence of the polyphagous leafminer pest Liriomyza sativae Blanchard in Bangladesh. DNA barcode sequences for mitochondrial COI were generated for Agromyzidae larvae, pupae and adults collected from field populations across Bangladesh. BLAST sequence similarity searches of GenBank showed 99-100% sequence similarity with populations of L. sativae Blanchard and 97% sequence similarity with L. trifolii (Burgess). Neighbor-joining distance analysis was used to investigate patterns of clustering of the Bangladeshi sequences with other pest species of Liriomyza. Sequences for the Bangladeshi specimens clustered unambiguously with L. sativae sequences from GenBank. This represents the first molecular confirmation of L. sativae in Bangladesh, and we conclude that this destructive leafminer is continuing its geographic expansion within Asia.

Keywords : Molecular taxonomy; DNA barcodes; Liriomyza sativae; Agromyzidae; Bangladesh

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About the article

Received: 2013-12-05

Accepted: 2014-06-14

Published Online: 2014-08-27


Citation Information: DNA Barcodes, ISSN (Online) 2299-1077, DOI: https://doi.org/10.2478/dna-2014-0005.

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© 2014 Sabrina Amin, et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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