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DNA Barcodes

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DNA barcoding of the vegetable Leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) in Bangladesh

Sabrina Amin
  • Corresponding author
  • Department of Biochemistry & Biotechnology, University of Science & Technology Chittagong, Bangladesh
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/ Sonja J. Scheffer / Matthew L. Lewis / Mostafa Kamal Pasha / Badrul Amin Bhuiya
Published Online: 2014-08-27 | DOI: https://doi.org/10.2478/dna-2014-0005


DNA barcoding revealed the presence of the polyphagous leafminer pest Liriomyza sativae Blanchard in Bangladesh. DNA barcode sequences for mitochondrial COI were generated for Agromyzidae larvae, pupae and adults collected from field populations across Bangladesh. BLAST sequence similarity searches of GenBank showed 99-100% sequence similarity with populations of L. sativae Blanchard and 97% sequence similarity with L. trifolii (Burgess). Neighbor-joining distance analysis was used to investigate patterns of clustering of the Bangladeshi sequences with other pest species of Liriomyza. Sequences for the Bangladeshi specimens clustered unambiguously with L. sativae sequences from GenBank. This represents the first molecular confirmation of L. sativae in Bangladesh, and we conclude that this destructive leafminer is continuing its geographic expansion within Asia.

Keywords : Molecular taxonomy; DNA barcodes; Liriomyza sativae; Agromyzidae; Bangladesh


  • [1] Parrella MP (1982) A review of the history and taxonomy of economically important serpentine leafminers (Liriomyza spp.) in California (Diptera: Agromyzidae). Pan-Pacific Entomologist 58: 302-308. Google Scholar

  • [2] Rauf A, Shepard BM & Johnson MW (2000) Leafminers in vegetables, ornamental plants and weeds in Indonesia: surveys of host crops, species composition and parasitoids. Int J Pest Managemt 46: 257-266. Google Scholar

  • [3] Andersen A, Nordhus E, Thang VT, An TTT, Hung HQ & Hofsvang T (2002) Polyphagous Liriomyza species (Diptera: Agromyzidae) in vegetables in Vietnam. Trop Agric 779: 241-246. Google Scholar

  • [4] Chen X, Lang F, Xu Z, He J & Ma Y (2003) The occurrence of leafminers and their parasitoids on vegetables and weeds in Hangzhou area, Southeast China. Biocontrol 48: 515-527. Google Scholar

  • [5] Spencer KA (1973) Agromyzidae (Diptera) of economic importance. Series Entomologica 9, Dr W Junk, The Hague. 418 pp. Google Scholar

  • [6] Johnson MW, Welter C, Toscano NC, Ting IP & Trumble JT (1983) Reduction of tomato leaflet photosynthesis rates by mining activity of Liriomyza sativae (Diptera: Agromyzidae). J Econ Entomol 76: 1061-1063. Google Scholar

  • [7] Bhuiya BA, Mazumdar S, Pasha MK, Islam W, Miah MI & Hossain MS (2010) A preliminary report on the agromyzid leaf miner pest attack on some agricultural crops and weeds in Bangladesh. J Taxon Biodiv Res 4: 47-50. Google Scholar

  • [8] Hebert PDN, Cywinska A, Ball SL & deWaard JR (2003) Biological identifications through DNA barcodes. Proc Biol Sci 270: 313-321. Google Scholar

  • [9] Hebert PDN, Penton EH, Burns JM, Janzen DH & Hallawachs W (2004a) Ten species in one: DNA barcoding reveals cryptic species in the Neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci USA 101: 14812-14817. Google Scholar

  • [10] Barrett RDH & Hebert PDN (2005) Identifying spiders through DNA barcodes. Can J Zool 83: 481-491. Google Scholar

  • [11] Palumbi SR, & Cipriano F (1998) Species identification using genetic tools: the value of nuclear and mitochondrial gene sequences in whale conservation. J Hered 89: 459-464. PubMedGoogle Scholar

  • [12] Symondson WOC (2002) Molecular identification of prey in predator diets. Mol Ecol 83: 137-147. Google Scholar

  • [13] Scheffer, S. J. 2001. Molecular evidence of cryptic species within the Pea Leafminer Liriomyza huidobrensis (Diptera: Agromyzidae). J Econ Entomol 93(4): 1146-1151. Google Scholar

  • [14] Scheffer SJ & Lewis ML (2005) Mitochondrial phylogeography of the vegetable pest Liriomyza sativae (Diptera: Agromyzidae): divergent clades and invasive populations. Ann Entomol Soc Am 98: 181-186. Google Scholar

  • [15] Scheffer SJ & Lewis ML (2006) Mitochondrial phylogeography of the vegetable pest Liriomyza trifolii (Diptera: Agromyzidae): diverged clades and invasive populations. Ann Entomol Soc Am 99: 991-998. Google Scholar

  • [16] Scheffer SJ, Lewis ML & Joshi RC (2006) DNA barcoding applied to invasive leafminers (Diptera: Agromyzidae) in the Philippines. Ann Entomol Soc Am 99(2): 204-210. CrossrefGoogle Scholar

  • [17] Spencer KA (1965) A clarification of status of Liriomyza trifolii and some related species (Diptera: Agromyzidae). Proc Entomol Soc Washington 67(1): 32-40. Google Scholar

  • [18] Dempewolf M (2004) Arthropods of economic importance - Agromyzidae of the World (CD-ROM), ETI, University of Amsterdam, Amsterdam. Google Scholar

  • [19] Shiao SF (2004) Morphological diagnosis of six Liriomyza species (Diptera: Agromyzidae) of quarantine importance in Taiwan. Appl Entomol Zool 39(1): 27–39. CrossrefGoogle Scholar

  • [20] Winkler IS, Scheffer SJ & Mitter C (2009) Molecular phylogeny and systematics of leaf-mining flies (Diptera: Agromyzidae): delimitation of Phytomyza Fallén sensu lato and included species groups, with new insights on morphological and host-use evolution. Systematic Entomology 34: 260–292. Web of ScienceGoogle Scholar

  • [21] Simon CF, Frati A, Beckenbach B, Crespi HL & Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87: 651-701. Google Scholar

  • [22] Folmer O, Black M, Hoeh W, Lutz R & Vrijenhoek R 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 3, 294-297. PubMedGoogle Scholar

  • [23] Altschul SF, Gish W, Miller W, Myers EW & Lipman DJ (1990) “Basic local alignment search tool.” J Mol Biol 215:403-410. Google Scholar

  • [24] Swofford DL (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. 144pp. Google Scholar

  • [25] Bhuiya BA, Amin S & Mazumdar S (2011) First report of vegetable leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) from Bangladesh. J Taxon Biodiv Res 5: 17-19. Google Scholar

  • [26] Singh S & Ipe IM (1973) The Agromyzidae from India. St. John’s College. 286pp. Google Scholar

  • [27] Iawasaki A, Kasugai K, Iwaizumi R & Sasakawa M (2000) A newly recorded pest, Liriomyza sativae Blanchard in Japan. Plant Protect 54: 142-147 (in Japaneese). Google Scholar

  • [28] Civelek HS (2002) New records of Agromyzidae (Diptera) from Western Turkey. Insecta Mundi 16(1-3): 48-55. Google Scholar

  • [29] Chen B & Kang L (2005) Implication of pupal cold tolerance for the northern overwintering range limit of the leafminer Liriomyza sativae (Diptera: Agromyzidae) in China. Applied Entomology and Zoology 40: 437-446. Google Scholar

  • [30] Tran TTA, Tran DH, Konishi K & Takagi M (2005) The vegetable leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) and its parasitoids on cucumber in the Hochiminh Region of Vietnam. J. of the Faculty of Agriculture, Kyushu University 50: 119-124.Google Scholar

About the article

Received: 2013-12-05

Accepted: 2014-06-14

Published Online: 2014-08-27

Citation Information: DNA Barcodes, Volume 2, Issue 1, ISSN (Online) 2299-1077, DOI: https://doi.org/10.2478/dna-2014-0005.

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© 2014 Sabrina Amin, et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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