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DNA Barcodes

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Estimating the effectiveness of species identification by sequencing of two chloroplast DNA loci (matK and rbcL) in selected groups of Polish flora

M. Combik
  • Corresponding author
  • Władysław Szafer Institute of Botany, Polish Academy of Sciences, Kraków
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Z. Mirek
  • Corresponding author
  • Władysław Szafer Institute of Botany, Polish Academy of Sciences, Kraków
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-06-19 | DOI: https://doi.org/10.1515/dna-2015-0003

Abstract

This paper focuses on assessing the effectiveness of species identification using barcoding standard loci (matK and rbcL) for a selected group of Polish flora. 55 samples of 17 species from six taxonomic groups representing various parts of the system, including Monocots and Dicots as well as Pteridophytes, were selected. PCR amplification success was 100% (of all samples) for the rbcL primer and 64% for matK primers giving in sum 87 fragments for sequencing. The aligned sequences show that in the Vaccinium, Dryopters and Symphytum species groups barcoding is very useful and makes it possible to identify most species in those groups. In the Symphytum genus only the rbcL locus shows differences between the species. In the case of Dryopteris due to problems with matK amplification only the rbcL locus could be analyzed, but it is highly useful in this group. Out of 17 species selected for this study, barcoding makes it possible to identify 11 of them, i.e. 64% of all. In the Anthoxanthum group, sequences of plastid loci show intraspecific variability which can be used to make phylogeographical analysis but could complicate species identification.

Keywords : barcoding effectiveness; polish flora; Monocots; Dicots; Pteridophytes; direct PCR

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About the article

Received: 2014-10-07

Accepted: 2015-02-03

Published Online: 2015-06-19


Citation Information: DNA Barcodes, ISSN (Online) 2299-1077, DOI: https://doi.org/10.1515/dna-2015-0003.

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© 2015 M. Combik, Z. Mirek. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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