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DNA Barcodes

Ed. by Mitchell, Andrew

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Emerging Science

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2299-1077
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Extending DNA barcoding coverage for Lake Whitefish (Coregonus clupeaformis) across the three major basins of Lake Huron

Lauren M. Overdyk
  • Corresponding author
  • Department of Integrative Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1
  • Email
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/ Heather E. Braid
  • Institute for Applied Ecology New Zealand, Auckland University of Technology, Private Bag 92006, Auckland, New Zealand 1010
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/ Stephen S. Crawford
  • Chippewas of Nawash, Unceded First Nation, 135 Lakeshore Blvd, Nevaashiinigmiing, Ontario, Canada N1G 2W1
  • Department of Integrative Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1
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  • De Gruyter OnlineGoogle Scholar
/ Robert H. Hanner
  • Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1
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Published Online: 2015-11-26 | DOI: https://doi.org/10.1515/dna-2015-0007

Abstract

DNA barcoding is a useful tool for both species identification and discovery, but the latter requires denser sampling than typically used in barcode studies. Lake Whitefish (Coregonus clupeaformis) is a valuable species, fished traditionally, commercially, and recreationally in Lake Huron. Based on the natural geographic and bathymetric separation of the three major basins in Lake Huron, the potential separation of Lake Whitefish within these basins, and the variation among life history (early and late spawning), we predicted that Lake Huron might harbour cryptic lineages of Lake Whitefish at the basin level. To test this prediction, DNA barcodes of the mitochondrial 5’ cytochrome c oxidase subunit I (COI) gene sequences were recovered from spawning phase Lake Whitefish (n = 5 per site), which were collected from sites (n = 28) around Lake Huron during Fall 2012. These sequences, combined with other publically available DNA barcodes from the Barcode of Life Data System (BOLD), revealed twelve unique haplotypes across North America, with seven unique to Lake Huron. The dominant haplotype was found throughout Lake Huron and east to the St. Lawrence River. No deep divergences were revealed. This comprehensive lake-wide sampling effort offers a new perspective on C. clupeaformis, and can provide insight for environmental assessments and fisheries management.

Keywords: haplotype; COI; Coregoninae

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About the article

Received: 2014-11-14

Accepted: 2015-06-08

Published Online: 2015-11-26

Published in Print: 2015-01-01


Citation Information: DNA Barcodes, ISSN (Online) 2299-1077, DOI: https://doi.org/10.1515/dna-2015-0007.

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