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Ecological Chemistry and Engineering S

The Journal of Society of Ecological Chemistry and Engineering

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The effect of tropospheric ozone on flavonoids and pigments content in common buckwheat cotyledons

Henryk Dębski
  • Siedlce University of Natural Sciences and Humanities, Faculty of Natural Sciences, ul. B. Prusa 12, 08-110 Siedlce, Poland
  • Other articles by this author:
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/ Wiesław Wiczkowski
  • Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Department of Chemistry and Biodynamics of Food, ul. J. Tuwima 10, 10-748 Olsztyn, Poland
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/ Dorota Szawara-Nowak
  • Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Department of Chemistry and Biodynamics of Food, ul. J. Tuwima 10, 10-748 Olsztyn, Poland
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/ Natalia Bączek
  • Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Department of Chemistry and Biodynamics of Food, ul. J. Tuwima 10, 10-748 Olsztyn, Poland
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/ Małgorzata Piechota
  • Siedlce University of Natural Sciences and Humanities, Faculty of Natural Sciences, ul. B. Prusa 12, 08-110 Siedlce, Poland
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/ Marcin Horbowicz
  • Corresponding author
  • Siedlce University of Natural Sciences and Humanities, Faculty of Natural Sciences, ul. B. Prusa 12, 08-110 Siedlce, Poland
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Published Online: 2017-10-11 | DOI: https://doi.org/10.1515/eces-2017-0031

Abstract

Tropospheric ozone forms in photochemical reactions or by refuse burning and combustion of exhaust gases from engines, and during some industrial processes. The mean ambient ozone concentration doubled during the last century, and in many urban areas has reached the phytotoxic level. In the present study, there was determined the effect of ozone fumigation on levels of individual flavonoids, chlorophylls, carotenoids and total phenols in the cotyledons of four common buckwheat cultivars (Hruszowska, Panda, Kora and Red Corolla). Six-day-old buckwheat seedlings were grown in controlled conditions and treated with an elevated dose of ozone (391 μg · m−3) during 5 days for 1 h each day. After the experiment, the cotyledons of the seedlings were analysed for individual flavonoids, chlorophylls, carotenoids and total phenols. Shoot elongation was also measured. Individual types of flavonoids in buckwheat cotyledons were found to respond to an elevated ozone dose in various ways. The response was also dependent on the cultivar evaluated. In the cotyledons of ozonized buckwheat seedlings, contents of C-glucosides of luteolin and apigenin decreased or did not change depending on the cultivar examined. In the case of flavonols, the contents of quercetin-3-O-rhamnosyl-galactoside and rutin (quercetin-3-O-rhamnosyl-glucoside) were markedly reduced in most cultivars. O3 had no effect on the level of anthocyanins and chlorophylls but it decreased carotenoids, and tended to inhibit buckwheat growth. In conclusion, a thesis can be formulated that, due to high reduction in important flavonoids, an elevated level of ambient ozone decreases the nutritional value of common buckwheat seedlings.

Keywords: common buckwheat; seedling; ozone; flavone; flavonol; chlorophyll; carotenoid

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About the article

Published Online: 2017-10-11

Published in Print: 2017-09-01


Citation Information: Ecological Chemistry and Engineering S, ISSN (Online) 1898-6196, DOI: https://doi.org/10.1515/eces-2017-0031.

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© 2017 Henryk Dębski et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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