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Folia Veterinaria

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Pathological Alterations in Hepatocytes of Dairy Cows With a Tendency to Emaciation and Fattening

J. Pivko
  • National Agricultural and Food Centre, Research Institute for Animal Production Nitra Hlohovecká 2, 951 41 Lužianky–Nitra The Slovak Republic
/ P. Makovický
  • Czech Centre for Phenogenomics, (BIOCEV), Laboratory of Transgenic Models of Diseases Institute of Molecular Genetics of the ASCR, v. v. i., Vídeňská 1083, 142 20 Prague The Czech Republic
/ A. Makarevich
  • Corresponding author
  • National Agricultural and Food Centre, Research Institute for Animal Production Nitra Hlohovecká 2, 951 41 Lužianky–Nitra The Slovak Republic
  • Email:
/ A. Sirotkin
  • National Agricultural and Food Centre, Research Institute for Animal Production Nitra Hlohovecká 2, 951 41 Lužianky–Nitra The Slovak Republic
/ P. Makovický
  • Selye Janos University in Komárno, Department of Biology, Bratislavská 3322, 945 01 Komárno The Slovak Republic
/ E. Kubovičová
  • National Agricultural and Food Centre, Research Institute for Animal Production Nitra Hlohovecká 2, 951 41 Lužianky–Nitra The Slovak Republic
Published Online: 2016-07-06 | DOI: https://doi.org/10.1515/fv-2016-0019


The aim of this study was to demonstrate the histochemical and histopathological alterations in the livers of cows with a tendency to become emaciated (body condition score - BCS1 and 2) and a tendency to become fattened (BCS4 and 5) in comparison to the cows of average body condition (BCS3) presented as a control. The histochemical analysis (PAS reaction) showed that the influence of emaciation and fattening in our study was manifested by a decreased occurrence of glycogen and a decreased level of the PAS-positive matter in the hepatocytes of dairy cows with BCS1, 2, 4 and 5. An abundant accumulation of lipids in the form of large lipid droplets, liposomes and lipoproteins observed in the hepatocytes of emaciated and fattened (BCS1 and 5) cows may be related to moderate-severe steatosis. These observations suggest a relationship between liver steatosis and the occurrence of lipoproteins in cows with a tendency toward emaciation and fattening.

Keywords: body condition score; hepatocyte; liver; steatosis


  • 1. Alexander, C. A., Hamilton, R. L., Havel, R. J., 1976: Subcellular localization of B apoprotein of plasma lipoproteins in rat liver. J. Cell Biol., 69, 241-263.Google Scholar

  • 2. Anantharaju, A., Feller, A., Chedid, A., 2002: Aging liver. Gerontology, 40, 343-353.Google Scholar

  • 3. Berger, H. M., Den Ouden, A. L, Calame, J. J., 1985: Pathogenesis of liver damage during parenteral nutrition: is lipofuscin a clue ? Arch. Dis. Child., 60, 774-776.Google Scholar

  • 4. Boshier, D. P., Holloway, H., Kitchin, L. F., 1984: A comparison of standard lipid staining techniques used in electron microscopic studies of mammalian tissues. Stain Technol., 59, 83.Google Scholar

  • 5. Brizzee, K. R., Ordy, J. M., 1981: Cellular feature, regional accumulation, and prospects of modification of age pigments in mammals. In Sohal, R.S. (Ed.): Age Pigments. Elsevier Biomedical Press, Amsterdam, Holland, 102-154.Google Scholar

  • 6. Brunt, E. M., Tiniakos, D. G., 2010: Histopathology of non-alcoholic fatty liver disease. World J. Gastroenterol., 16, 5286-5296.CrossrefGoogle Scholar

  • 7. Cakala, S., Bieniek, K., 1975: Bromsulphatalein clearance and total billirubin level in cow deprived of food and water. Zbl. Vet. Med. A, 22, 605-610.Google Scholar

  • 8. Edmonson, A. J., Lean, I. J., Weaver, L. D., Farver, T., Webster, G. 1989: A body condition scoring chart for Holstein dairy cows. J. Dairy Sci., 72, 68-78.Google Scholar

  • 9. Enwonwu, C. O., Stambaugh, R., Sreebny, L., 1971: Synthesis and degradation of liver ribosomal RNA in fed and fasted rats. J. Nutr., 101, 337-346.Google Scholar

  • 10. Fon Tacer, K., Rozman, D., 2011: Nonalcoholic fatty liver disease: focus on lipoprotein and lipid deregulation. J. Lipids, 2011, 783-976.Google Scholar

  • 11. Gumulec, J., Masařík, M., Křížová, S., Babula, P., Hrabec, R., Rovný, A., Masaříková, M., Kizek, R., 2011: Molecular mechanisms of zinc in prostate cancer. Klin. Onkol., 24, 249-255.Google Scholar

  • 12. Hogstrand, C., Kille, P., Nicholson, R. I., 2009: Zinc transporters and cancer: a potential role for ZIP7 as a hub for tyrosine kinase activation. Trends Mol. Med., 15, 101-111.Web of ScienceGoogle Scholar

  • 13. Iwata, H., Tanaka, H., Kanke, T., Sakaguchi, Y., Shibano, K., Kuwayama, T., Monji, Y., 2010: Follicle growth and oocyte developmental competence in cows with liver damage. Reprod. Dom. Anim., 45, 888-895.Google Scholar

  • 14. Jean, G., Lambertenghi, G., Ranzi, T., 1968: Ultrastructural study of the liver in hepatic porphyria. J. Clin. Pathol., 21, 501-507.CrossrefGoogle Scholar

  • 15. Kuntz, E, Kuntz, H. D., 2002: Hepatology. Principles and Practice. Springer-Verlag, Heidelberg, 825 pp.Google Scholar

  • 16. Lehninger, A. L., 1972: Lipids, Lipoproteins and Membranes. Biosynthesis of Lipids. Chapter 23. In Lehninger, A. L. (ed.): Biochemistry. Worth Publisher, Inc., New York, 202-532.Google Scholar

  • 17. Li, M., Song, J., Mirkov, S., Xiao, S. Y., Hart, J., Liu, W., 2011: Comparing morphometric, biochemical and visual measurements of macrovesicular steatosis of liver. Hum. Pathol., 42, 356-360.Web of ScienceGoogle Scholar

  • 18. Mak, K. M., Kwong, A. J., Chu, E., Hoo, N. M., 2012: Hepatic steatosis, fibrosis and cancer in elderly cadavers. Anat. Rec., 295, 40-50.Web of ScienceGoogle Scholar

  • 19. Makovicky, P., Dudova, M., Tumova, E., Rajmon, R., Vodkova, Z., 2011: Experimental study of non-alcoholic fatty liver disease (NAFLD) on a model of starving chickens: is generalization of steatosis accompanied by fibrosis of the liver tissue ? Pathol. Res. Pract., 207, 151-155.Web of ScienceGoogle Scholar

  • 20. März, W., Scharnagl, H., Mondorf, U., Wieland, H., Gross, W., Kostner, G. M., 1996: The receptor-mediated endocytosis of lipoprotein(a). Z. Gastroenterol., 34, 131-138.Google Scholar

  • 21. Pivko, J., Makarevich, A. V., Kubovičová, E., Ostro, A., Hegedušová, Z., Louda, F., 2012: Histopathological alterations in the antral ovarian follicles in dairy cows with a tendency to emaciation. Histol. Histopathol., 27, 1211-1217.Google Scholar

  • 22. Reid, I. M., Isenor, R. N., 1972: Effect of starvation on molecular bodies and rough endoplasmic reticulum in the bovine hepatocytes. Exp. Cell Res., 75, 282-285.Google Scholar

  • 23. Reid, I. M., Collins, R. A., 1980: The pathology of postparturient fatty liver in high-yielding dairy cows. Invest. Cell. Pathol., 3, 237-249.Google Scholar

  • 24. Reid, I. M., Roberts, C. J., Baird, G. D., 1980: The effects of underfeeding during pregnancy and lactation on structure and chemistry of bovine liver and muscle. J. Agri. Sci., 94, 239-245.Google Scholar

  • 25. Savio, J. D., Boland, M. P., Roche, J. P., 1990: Development of dominant follicles and length of ovarian cycles in postpartum dairy cows. J. Reprod. Fert., 88, 581-591.Google Scholar

  • 26. Schmucker, D. L., 2005: Age-related changes in liver structure and function: implications for disease ? Exp. Gerontol., 40, 650-659.Google Scholar

  • 27. Silke, V., Diskin, M. G., Kenny, D. A., Boland, M. P., Dillon, P., Mee, J. F., Sreenan, J. M., 2002: Extent, pattern and factors associated with late embryonic loss in dairy cows. Anim. Reprod. Sci., 71, 1-12.Google Scholar

  • 28. Sirotkin, A. V., Makarevich, A. V., Makovicky, P., Kubovicova, E., 2013: Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores. J. Anim. Feed Sci., 22, 316-322.Google Scholar

  • 29. Yamasaki, S. K., Sakata-Sogawa, A., Hasegawa, T., Suzuki, K., Kabu, E., Sato, T., et al., 2007: Zinc is a novel intracellular second messenger. J. Cell Biol., 177, 637-645.Web of ScienceGoogle Scholar

  • 30. Zelber-Sagi, S., Ratziu, V., Oren, R., 2011: Nutrition and physical activity in NAFLD: An overview of the epidemiological evidence. World J. Gastroenterol., 17, 3377-3389. Web of ScienceCrossrefGoogle Scholar

About the article

Received: 2016-05-11

Published Online: 2016-07-06

Published in Print: 2016-06-01

Citation Information: Folia Veterinaria, ISSN (Online) 2453-7837, DOI: https://doi.org/10.1515/fv-2016-0019.

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© 2016. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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