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Helminthologia

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Genetic structuring and differentiation of Echinococcus multilocularis in Slovakia assessed by sequencing and isoenzyme studies

V. Šnábel
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • :
/ M. Miterpáková
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • :
/ S. D’Amelio
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • Department of Sciences of Public Health, Parasitology Section, University of Rome „La Sapienza“, Piazzale Aldo Moro 5, 00185, Rome, Italy
  • :
/ M. Busi
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • Department of Sciences of Public Health, Parasitology Section, University of Rome „La Sapienza“, Piazzale Aldo Moro 5, 00185, Rome, Italy
  • :
/ D. Bartková
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • :
/ L’. Turčeková
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • :
/ C. Maddox-Hyttel
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, Bülowsvej 27, DK-1790, Copenhagen V, Denmark
  • :
/ P. Skuce
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, Scotland, UK
  • :
/ P. Dubinský
  • Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia
  • :
Published Online: 2006-12-01 | DOI: https://doi.org/10.2478/s11687-006-0037-6

Abstract

Nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene and isoenzyme analysis were used to survey the genetic variability in Echinococcus multilocularis populations from Slovakia. A sample of 12 isolates acquired from 10 different districts from red foxes exhibited identical sequences. Compared with the previously described E. multilocularis variants, one base substitution was consistently observed relative to the M1 variant (detected in China, Alaska, North America, Japan) and three base substitutions were recorded relative to the M2 variant (detected in Germany) in the CO1 fragment. These data, along with the recently gathered data from French isolates, are indicative of a genetically unique population occurring in Central and Western Europe. Electrophoretic examination of enzymes produced by 14 gene loci revealed intraspecific polymorphism only with the glucose-phosphate isomerase (two distinct patterns) and the mannosephosphate isomerase (four genotypes composed of three alleles) enzyme systems. To allow a fast species differentiation of E. multilocularis and E. granulosus (specifically, the G7 genotype occurring in Slovakia), discriminative electrophoretic characters between the species were obtained by isoenzyme analysis. Fixed genetic differences between the species were detected in the glucose-phosphate isomerase, esterase and aldolase systems, and partial differences were detected in four additional systems.

Keywords: Echinococcus multilocularis; polymorphism; DNA; isoenzymes; Slovakia

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Published Online: 2006-12-01

Published in Print: 2006-12-01


Citation Information: Helminthologia. Volume 43, Issue 4, Pages 196–202, ISSN (Online) 1336-9083, ISSN (Print) 0440-6605, DOI: https://doi.org/10.2478/s11687-006-0037-6, December 2006

© 2006 Institute of Parasitology, SAS, Košice, Slovakia. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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