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Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus

WPŁYW KWASU GIBERELINOWEGO, STRATYFIKACJI ORAZ ZASOLENIA NA KIEŁKOWANIE NASION ECHINACEA PURPUREA CV. MAGNUS

Shamila Yadolahi Zadeh
  • Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
  • Other articles by this author:
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/ Ali Akbar Ramin
  • Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Bahram Baninasab
  • Corresponding author
  • Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-09-23 | DOI: https://doi.org/10.1515/hepo-2015-0019

Summary

This study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpurea cv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1 GA3 with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1 GA3 for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3 showed higher viability and better performance under salinity stress condition.

Streszczenie

Prezentowane badania obejmowały dwa odrębne doświadczenia, mające na celu określenie warunków przełamywania spoczynku nasion jeżówki purpurowej (Echinacea purpurea cv. Magnus) oraz wpływu zasolenia na ich kiełkowanie. W pierwszym z eksperymentów zastosowano pięć poziomów stężeń kwasu giberelinowego GA3 (0, 250, 500, 1000 i 1500 mg×L−1) w połączeniu z chłodną stratyfikacją nasion trwającą odpowiednio: 0, 5, 10 i 15 dni, i przebiegającą w wilgotnym podłożu w temperaturze 5°C. Doświadczenie czynnikowe przeprowadzono w układzie całkowicie losowym, w czterech powtórzeniach. Analizy statystyczne wykazały, że zastosowanie dziesięciodniowej chłodnej stratyfikacji w połączeniu z GA3 o stężeniu 250 mg×L1 istotnie zwiększa odsetek kiełkujących nasion i skraca średni czas kiełkowania. W drugim eksperymencie nasiona jeżówki były przechowywane przez 10 dni w temperaturze 5°C i połowę z nich poddano przez 24 godziny działaniu roztworu GA3 o stężeniu 250 mg×L−1. Dla obu grup nasion badano wpływ stresu solnego (działania NaCl). Powyższe doświadczenie prowadzono w układzie całkowicie losowym, w czterech powtórzeniach, stosując pięć poziomów zasolenia (0, 20, 40, 60 i 80 mM NaCl). Otrzymane wyniki wskazują, że jeżówka purpurowa, na etapie kiełkowania nasion, jest bardzo wrażliwa na zasolenie. Zwiększanie jego poziomu powodowało spadek ilości kiełkujących nasion i dobrze wykształconych siewek oraz wzrost średniego czasu kiełkowania. Większą odporność w warunkach stresu solnego wykazywały nasiona poddane działaniu GA3.

Keywords: purple coneflower; chilling; GA3; NaCl

Słowa kluczowe:: jeżówka purpurowa; chłodna stratyfikacja; GA3; NaCl

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About the article

Published Online: 2015-09-23

Published in Print: 2015-09-01


Citation Information: Herba Polonica, ISSN (Online) 2449-8343, DOI: https://doi.org/10.1515/hepo-2015-0019.

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