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Wood Research and Technology

Holzforschung

Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Faix, Oskar / Salmén, Lennart

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

12 Issues per year


IMPACT FACTOR 2017: 2.079

CiteScore 2017: 1.94

SCImago Journal Rank (SJR) 2017: 0.709
Source Normalized Impact per Paper (SNIP) 2017: 0.979

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1437-434X
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Volume 55, Issue 6

Issues

Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls

G. Koch / G. Kleist
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/HF.2001.091

Summary

The localisation of lignin and phenolic extractives in woody tissue was determined using scanning UV microspectrophotometry. This improved cellular analytical technique enabled direct imaging of the topochemical lignin distribution within individual cell wall layers with a resolution of 0.25 μm2. Selected softwood (Picea abies), hardwood (Fagus sylvatica, Entandrophragma cylindricum, Prunus serotina) and monocotyledon (Phyllostachys edulis) sections of 1 μm thickness were scanned at a fixed wavelength and evaluated with the “APAMOS” software. This approach allowed the distribution pattern of lignins and aromatic extractives within the cell wall to be visualised simultaneously. The method was found to be ideally suited to the study of their subcellular distribution in plant cell walls.

About the article

Published Online: 2005-06-01

Published in Print: 2001-11-06


Citation Information: Holzforschung, Volume 55, Issue 6, Pages 563–567, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/HF.2001.091.

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