Jump to ContentJump to Main Navigation
Show Summary Details
More options …
Wood Research and Technology

Holzforschung

Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Salmén, Lennart

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi


IMPACT FACTOR 2018: 2.579

CiteScore 2018: 2.43

SCImago Journal Rank (SJR) 2018: 0.829
Source Normalized Impact per Paper (SNIP) 2018: 1.082

Online
ISSN
1437-434X
See all formats and pricing
More options …
Volume 59, Issue 5

Issues

Comparison of quantitative real-time PCR, chitin and ergosterol assays for monitoring colonization of Trametes versicolor in birch wood

Morten Eikenes / Ari M. Hietala / Gry Alfredsen / Carl Gunnar Fossdal / Halvor Solheim
Published Online: 2005-08-08 | DOI: https://doi.org/10.1515/HF.2005.093

Abstract

This paper describes the use of quantitative real-time PCR for monitoring colonization of birch wood (Betula pubescens) by the white-rot fungus Trametes versicolor in an EN113 decay experiment. The wood samples were harvested after 4, 8, 12, 16 and 20 weeks of incubation. The mass loss was in the range of 4–40%. Chitin and ergosterol assays were conducted for comparison. Second-order polynomial fits of the mass loss of decayed wood versus chitin, ergosterol and DNA gave correlations (r2) of 0.87, 0.61 and 0.84, respectively. Compared to the other two assays employed, real-time PCR data correlated best with the relative mass loss of decayed samples 4–8 weeks after inoculation, while the saturation and decline of DNA-based estimates for fungal colonization 16–20 weeks after inoculation indicated that the DNA assay is not suited for quantification purposes in the late stages of decay. The impact of conversion factors, extraction efficiency, inhibitory compounds and background levels in relation to the three detection assays used is discussed.

Keywords: birch; chitin; ergosterol; quantitative real-time PCR; Trametes versicolor; wood mass loss.

About the article

Corresponding author. Norwegian Forest Research Institute, Høgskoleveien 8, 1432 Ås, Norway Tel.: +47-64 949000 Fax: +47-64 942980


Received: February 14, 2005

Accepted: June 1, 2005

Published Online: 2005-08-08

Published in Print: 2005-09-01


Citation Information: Holzforschung, Volume 59, Issue 5, Pages 568–573, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/HF.2005.093.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Josep Anton Mir-Tutusaus, Eloi Parladé, Marta Villagrasa, Damià Barceló, Sara Rodríguez-Mozaz, Maira Martínez-Alonso, Núria Gaju, Montserrat Sarrà, and Glòria Caminal
Journal of Biological Engineering, 2019, Volume 13, Number 1
[2]
Prithvi S. Shirahatti, Ramith Ramu, S. Nanjunda Swamy, Joseph Jeyabalaji Kingston, and M. N. Nagendra Prasad
Indian Phytopathology, 2019, Volume 72, Number 1, Page 139
[3]
G. Llorens-Blanch, E. Parladé, M. Martinez-Alonso, N. Gaju, G. Caminal, and P. Blánquez
Waste Management, 2017
[4]
Marina Badia-Fabregat, Daniel Lucas, Tero Tuomivirta, Hannu Fritze, Taina Pennanen, Sara Rodríguez-Mozaz, Damià Barceló, Glòria Caminal, and Teresa Vicent
Science of The Total Environment, 2017, Volume 579, Page 366
[5]
Hugh Cross, Jørn Henrik Sønstebø, Nina E. Nagy, Volkmar Timmermann, Halvor Solheim, Isabella Børja, Håvard Kauserud, Tor Carlsen, Barbara Rzepka, Katarzyna Wasak, Adam Vivian-Smith, and Ari M. Hietala
New Phytologist, 2017, Volume 213, Number 3, Page 1405
[6]
Fabian Weihmann, Iris Eisermann, Rayko Becher, Jorrit-Jan Krijger, Konstantin Hübner, Holger B. Deising, and Stefan G. R. Wirsel
BMC Microbiology, 2016, Volume 16, Number 1
[7]
U. Råberg, N. Terziev, and G. Daniel
International Wood Products Journal, 2014, Volume 5, Number 2, Page 83
[8]
G. Alfredsen, R. Ringman, A. Pilgård, and C. G. Fossdal
International Wood Products Journal, 2015, Volume 6, Number 1, Page 5
[9]
César Segovia, Alix Sauget, Arnaud Besserer, Raphaël Kueny, and Antonio Pizzi
Industrial Crops and Products, 2016, Volume 83, Page 438
[10]
Isabella Børja, Gry Alfredsen, Tore Filbakk, and Carl Gunnar Fossdal
PeerJ, 2015, Volume 3, Page e887
[11]
Guillem Llorens-Blanch, Marina Badia-Fabregat, Daniel Lucas, Sara Rodriguez-Mozaz, Damià Barceló, Taina Pennanen, Gloria Caminal, and Paqui Blánquez
Environ. Sci.: Processes Impacts, 2015, Volume 17, Number 2, Page 429
[12]
J.M.R. Apollo Arquiza and Jean Hunter
International Journal of Food Microbiology, 2014, Volume 187, Page 50
[13]
Zewei Song, Andrew Vail, M.J. Sadowsky, and Jonathan S. Schilling
Fungal Ecology, 2014, Volume 7, Page 39
[14]
Lars Wadsö, Sanne Johansson, Annica Pilgård, and Gry Alfredsen
Engineering in Life Sciences, 2013, Volume 13, Number 6, Page 536
[15]
H. Wallander, A. Ekblad, D.L. Godbold, D. Johnson, A. Bahr, P. Baldrian, R.G. Björk, B. Kieliszewska-Rokicka, R. Kjøller, H. Kraigher, C. Plassard, and M. Rudawska
Soil Biology and Biochemistry, 2013, Volume 57, Page 1034
[16]
Outi Niemenmaa, Sari Galkin, and Annele Hatakka
International Biodeterioration & Biodegradation, 2008, Volume 62, Number 2, Page 125
[17]
Annica Pilgård, Gry Alfredsen, Charlotte G. Björdal, Carl Gunnar Fossdal, and Isabella Børja
Holzforschung, 2011, Volume 65, Number 6
[19]
Outi Niemenmaa, Antti Uusi-Rauva, and Annele Hatakka
Biodegradation, 2008, Volume 19, Number 4, Page 555

Comments (0)

Please log in or register to comment.
Log in