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Wood Research and Technology


Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Salmén, Lennart

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

IMPACT FACTOR 2018: 2.579

CiteScore 2018: 2.43

SCImago Journal Rank (SJR) 2018: 0.829
Source Normalized Impact per Paper (SNIP) 2018: 1.082

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Volume 59, Issue 6


Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi

Ulrika Råberg / Nils O.S. Högberg
  • Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Carl Johan Land


In the present work PCR technology was used as a tool to detect the early stages of wood decay and was compared with microscopic evaluation. The wood decay fungi Postia placenta and Coniophora puteana were detectable in interior wood samples by terminal restriction fragment length polymorphism (T-RFLP) after 2weeks of incubation with monocultures, while microscopic detection of hyphae was not possible until after 7 weeks. A potential problem when fungal communities are studied with T-RFLPs of rDNA is that intra-specific variation complicates data analysis. In this work, we show that intra-specific sequence variation in the internal transcribed spacer of the rDNA in Coniophora puteana allows T-RFLP identification of this species. This is due to intra-specific variations in fragment length, in combination with the absence of point mutations in the selected restriction sites.

Keywords: brown rot; Coniophora; fungal colonisation; fungi; polymerase chain reaction (PCR); sequencing; terminal restriction fragment length polymorphism (T-RFLP); wood

About the article

Corresponding author. Department of Wood Science, Box 7008, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden

Received: April 25, 2005

Accepted: July 8, 2005

Published in Print: 2005-11-01

Citation Information: Holzforschung, Volume 59, Issue 6, Pages 696–702, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/HF.2005.111.

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