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Holzforschung

International Journal of the Biology, Chemistry, Physics, and Technology of Wood

Editor-in-Chief: Faix, Oskar

Editorial Board Member: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Salmen, Lennart / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

12 Issues per year


IMPACT FACTOR 2016: 1.868
5-year IMPACT FACTOR: 1.875

CiteScore 2016: 1.83

SCImago Journal Rank (SJR) 2015: 0.817
Source Normalized Impact per Paper (SNIP) 2015: 0.954

Online
ISSN
1437-434X
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Volume 60, Issue 6 (Nov 2006)

Issues

Morphological and chemical characterisation of the G-layer in tension wood fibres of Populus tremula and Betula verrucosa: Labelling with cellulose-binding module CBM1Hj Cel7A and fluorescence and FE-SEM microscopy

Geoffrey Daniel
  • WURC, Department of Wood Science, Swedish University of Agricultural Sciences, Uppsala, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lada Filonova
  • WURC, Department of Wood Science, Swedish University of Agricultural Sciences, Uppsala, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Åsa M. Kallas
  • Department of Biotechnology, Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tuula T. Teeri
  • Department of Biotechnology, Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2006-12-04 | DOI: https://doi.org/10.1515/HF.2006.104

Abstract

The gelatinous layer (G-layer) formed on the lumen wall in early- and latewood fibres of poplar and birch tension wood was characterised using a novel molecular marker specific for crystalline cellulose in conjunction with fluorescence and FE-SEM microscopy. Crystalline cellulose was localised using a cloned Cel7A cellulose-binding module (CBM1 Hj Cel7A) from the fungus Hypocrea jecorina conjugated directly to FITC/TRITC or indirectly via a secondary antibody conjugated to FITC for fluorescence microscopy or to gold/silver for FE-SEM. With the CBM1 Hj Cel7A conjugate, the G-layer was clearly distinguished from other secondary cell-wall layers as a bright green layer visible in fibres of tension wood in fluorescence microscopy. FEM-SEM images revealed the supramolecular architecture of the G-layer of poplar wood, which consists of well-defined, often concentrically orientated, cellulose aggregates of the order of 30–40 nm. The cellulose aggregates typically have a microfibril angle of almost 0°. Studies on cellulose marked with CBM1 Hj Cel7A followed by Au labelling and Ag enhancement complemented the fluorescence observations. The studies demonstrate the usefulness of this novel molecular marker for crystalline cellulose in situ, which was previously difficult to localise. Further proof of distinct cellulose aggregates was observed.

Keywords: carbohydrate-binding module (CBM); carbohydrate-binding module of family 1 labelled with fluorescein isothiocyanate or tetramethylrhodamine isothiocyanate (CBM1-FITC/TRITC); CBM1HjCel7A from Hypocrea jecorina Cel7A; cellulose aggregates; crystalline cellulose; field emission scanning electron microscopy (FE-SEM); gelatinous layer (G-layer); tension wood

About the article

Corresponding author. WURC, Department of Wood Science, Swedish University of Agricultural Sciences, P.O. Box 7008, 75007 Uppsala, Sweden


Received: March 28, 2006

Accepted: August 16, 2006

Published Online: 2006-12-04

Published in Print: 2006-11-01


Citation Information: Holzforschung, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/HF.2006.104.

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