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Rio, Jose C. / Laine, Christiane / Potthast, Antje / Takano, Toshiyuki / Theliander, Hans

Holzforschung

International Journal of the Biology, Chemistry, Physics, and Technology of Wood

Editor-in-Chief: Faix, Oskar

Editorial Board Member: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Salmen, Lennart / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Tamminen, Tarja / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

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Online
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1437-434X
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Volume 61, Issue 4 (Jun 2007)

Issues

Molar mass determination of lignins by size-exclusion chromatography: towards standardisation of the method

Stéphanie Baumberger
  • 1UMR 206 Chimie Biologique AgroParisTech, INRA, Institut National Agronomique Paris-Grignon, Thiverval-Grignon, France
/ Alfred Abaecherli
  • 2International Lignin Institute, Lausanne, Switzerland
/ Mario Fasching
  • 3Kompetenzzentrum Holz GmbH, c/o Lenzing AG, Austria
/ Gäoran Gellerstedt
  • 4Royal Institute of Technology, KTH, Stockholm, Sweden
/ Richard Gosselink
  • 5Wageningen UR, Agrotechnology and Food Sciences Group, Wageningen, The Netherlands
/ Bo Hortling
  • 6KCL, Helsinki, Finland
/ Jiebing Li
  • 7Royal Institute of Technology, KTH, Stockholm, Sweden
/ Bodo Saake
  • 8Institute for Wood Chemistry and Chemical Technology of Wood, Federal Research Centre for Forestry and Forest Products, Hamburg, Germany
/ Ed de Jong
  • 9Wageningen UR, Agrotechnology and Food Sciences Group, Wageningen, The Netherlands
Published Online: 2007-06-12 | DOI: https://doi.org/10.1515/HF.2007.074

Abstract

The reactivity and physicochemical properties of lignins are partly governed by their molar mass distribution. The development of reliable standard methods for determination of the molar mass distribution is not only relevant for designing technical lignins for specific applications, but also for monitoring and elucidating delignification and pulping processes. Size-exclusion chromatography (SEC) offers many advantages, such as wide availability, short analysis time, low sample demand, and determination of molar mass distribution over a wide range. A collaborative study has been undertaken within the “Eurolignin” European thematic network to standardise SEC analysis of technical lignins. The high-molar-mass fraction of polydisperse lignins was shown to be the main source of intra- and interlaboratory variations, depending on the gel type, elution solvent, detection mode, and calculation strategy. The reliability of two widespread systems have been tested: one based on alkali and a hydrophilic gel (e.g., TSK Toyopearl gel) and the other based on THF as solvent and polystyrene-based gels (e.g., Styragel). A set of practical recommendations has been deduced.

Keywords: molar mass determination; round robin; size exclusion chromatography; standardisation; technical lignins

About the article

Corresponding author. UMR 206 Chimie Biologique AgroParisTech, INRA, Institut National Agronomique Paris-Grignon, 78850 Thiverval-Grignon, France.


Received: 2006-01-25

Accepted: 2007-04-04

Published Online: 2007-06-12

Published in Print: 2007-06-01


Citation Information: Holzforschung, ISSN (Online) 1437434X, ISSN (Print) 00183830, DOI: https://doi.org/10.1515/HF.2007.074. Export Citation

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