Jump to ContentJump to Main Navigation
Show Summary Details
More options …
Wood Research and Technology

Holzforschung

Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Faix, Oskar / Salmén, Lennart

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

12 Issues per year


IMPACT FACTOR 2017: 2.079

CiteScore 2017: 1.94

SCImago Journal Rank (SJR) 2017: 0.709
Source Normalized Impact per Paper (SNIP) 2017: 0.979

Online
ISSN
1437-434X
See all formats and pricing
More options …
Volume 63, Issue 6

Issues

Comparing soluble Trametes pubescens laccase and cross-linked enzyme crystals (CLECs) for enzymatic modification of cellulose 10th EWLP, Stockholm, Sweden, August 25–28, 2008

Ilabahen Patel
  • Department of Chemistry, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Department of Food Sciences and Technology, Division of Food Biotechnology, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Roland Ludwig
  • Department of Food Sciences and Technology, Division of Food Biotechnology, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Research Center Applied Biocatalysis, Graz, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kitti Mueangtoom
  • Department of Food Sciences and Technology, Division of Food Biotechnology, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Research Center Applied Biocatalysis, Graz, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dietmar Haltrich
  • Department of Food Sciences and Technology, Division of Food Biotechnology, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Thomas Rosenau
  • Department of Chemistry, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Antje Potthast
  • Department of Chemistry, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Vienna, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2009-11-15 | DOI: https://doi.org/10.1515/HF.2009.073

Abstract

Three types of preparations – enzyme immobilized on Eupergit C, cross-linked enzyme crystals (CLECs) and lyophilized enzyme – have been obtained from Trametes pubescens laccase. Their activity in organic solvents has been comparatively evaluated, whereby the CLECs showed a significantly higher activity compared to the immobilized and the lyophilized variant. The soluble, lyophilized laccase and the CLECs were compared for their activity in the oxidation of cellulose in the laccase/TEMPO system. The “double heterogeneous” CLEC system – both the CLECs and the cellulose substrates are solids and only the mediator is homogeneously dissolved – showed similar reactivity to the conventional enzyme system. Laccase CLECs, being a solid, robust and highly active immobilized enzyme form can be conveniently used to modify (protected) low-molecular weight carbohydrates or cellulosics also in (aqueous-)organic media, and they offer many practical advantages: operational stability and ease of recycling coupled with high volumetric productivity.

Keywords: cellulose; cross-linked enzyme crystals (CLECs); gel permeation chromatography (GPC); laccase; laccase-mediator system (LMS); TEMPO oxidation

About the article

Corresponding author. Department of Chemistry, University of Natural Resources and Applied Life Sciences Vienna (BOKU), Muthgasse 18, A-1190 Vienna, Austria Phone: +43-1-36006 6071 Fax: +43-1-36006 6059


Received: 2008-12-03

Accepted: 2009-02-11

Published Online: 2009-11-15

Published Online: 2009-07-24

Published in Print: 2009-11-01


Citation Information: Holzforschung, Volume 63, Issue 6, Pages 715–720, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/HF.2009.073.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Hubert Hettegger, Hassan Amer, Nele S. Zwirchmayr, Markus Bacher, Takashi Hosoya, Antje Potthast, and Thomas Rosenau
Cellulose, 2018
[2]
Muhammad Bilal, Muhammad Asgher, Roberto Parra-Saldivar, Hongbo Hu, Wei Wang, Xuehong Zhang, and Hafiz M.N. Iqbal
Science of The Total Environment, 2017, Volume 576, Page 646
[3]
Muhammad Asgher, Muhammad Shahid, Shagufta Kamal, and Hafiz Muhammad Nasir Iqbal
Journal of Molecular Catalysis B: Enzymatic, 2014, Volume 101, Page 56
[4]
Melissa Guazzaroni, Marina Pasqualini, Giorgia Botta, and Raffaele Saladino
ChemCatChem, 2012, Volume 4, Number 1, Page 89
[5]
Ilabahen Patel, Roland Ludwig, Dietmar Haltrich, Thomas Rosenau, and Antje Potthast
Holzforschung, 2011, Volume 65, Number 4

Comments (0)

Please log in or register to comment.
Log in