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Holzforschung

International Journal of the Biology, Chemistry, Physics, and Technology of Wood

Editor-in-Chief: Faix, Oskar

Editorial Board Member: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Salmen, Lennart / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

12 Issues per year


IMPACT FACTOR 2016: 1.868
5-year IMPACT FACTOR: 1.875

CiteScore 2016: 1.83

SCImago Journal Rank (SJR) 2015: 0.817
Source Normalized Impact per Paper (SNIP) 2015: 0.954

Online
ISSN
1437-434X
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Volume 66, Issue 1 (Jan 2012)

Issues

Development of DNA-based methods to identify CITES-protected timber species: a case study in the Meliaceae family

Aki Michael Höltken / Hilke Schröder
  • Johann Heinrich von Thünen-Institute (vTI), Institute of Forest Genetics, Sieker Landstraße 2, D-22927 Großhansdorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Niko Wischnewski
  • University of Hamburg, Department of Wood Biology and Technology, Leuschnerstraße 91, D-21031 Hamburg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Bernd Degen
  • Johann Heinrich von Thünen-Institute (vTI), Institute of Forest Genetics, Sieker Landstraße 2, D-22927 Großhansdorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Elisabeth Magel
  • University of Hamburg, Department of Wood Biology and Technology, Leuschnerstraße 91, D-21031 Hamburg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Matthias Fladung
  • Johann Heinrich von Thünen-Institute (vTI), Institute of Forest Genetics, Sieker Landstraße 2, D-22927 Großhansdorf, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2011-08-19 | DOI: https://doi.org/10.1515/HF.2011.142

Abstract

Violation of CITES regulations in tropical timber trade necessitates the development of accurate species identification systems. The application of available methods, mostly based on visual illustrations and descriptions of wood anatomical characteristics, proved to be difficult or even impossible, particularly on lower taxonomic levels. Further, because most of the chain-of-custody documents are externally applied marks which can easily be manipulated, control methods should be based on wood features which are inherent in the wood itself. In a case study on five closely-related genera of the Meliaceae (mahogany) family, including Swietenia sp. (listed on CITES appendix II), Khaya, Entandrophragma, and Carapa sp. (legal trade timbers), this study demonstrates the process of developing DNA markers for identification purposes. A detailed sequence analysis of several non-coding cpDNA regions resulted in an assay of seven genus-specific SNP (single nucleotide polymorphism) markers. Tools have been designed that could be applied with low-cost equipment on the basis of PCR-RFLPs without the need for sequencing or capillary electrophoresis techniques. In addition, the application of the method to wood material with degraded DNA of low overall quantity is highlighted.

Keywords: barcoding; genetic marker; illegal logging; mahogany; wood identification

About the article

Corresponding author. University of Hamburg, Department of Wood Biology and Technology, Leuschnerstraße 91, D-21031 Hamburg, Germany


Received: 2011-03-17

Accepted: 2011-07-18

Published Online: 2011-08-19

Published in Print: 2012-01-01


Citation Information: Holzforschung, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/HF.2011.142.

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