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Rio, Jose C. / Laine, Christiane / Potthast, Antje / Takano, Toshiyuki / Theliander, Hans


International Journal of the Biology, Chemistry, Physics, and Technology of Wood

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Volume 66, Issue 3 (Mar 2012)


Morphological responses of Betula protoplasts in fiber spinning

Tomoko Seyama
  • Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
/ Tetsuo Kondo
  • Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
  • Email:
Published Online: 2011-09-30 | DOI: https://doi.org/10.1515/hf.2011.158


In a previous article, it was reported that, under stress conditions caused by Ca2+ ions, isolated protoplasts of Betula platyphylla leaves release a bundle of hollow fibrils as a stress-induced response. In the present article, details of this phenomenon have been investigated. As a special method, light microscopy combined with a ‘through focus variation’ technique was applied. In a regular culture medium, protoplasts could regenerate the cell walls, whereas protoplasts submitted to Ca2+ stress could not. Under stress conditions, the protoplasts inflated and formed a huge vacuole and, after 28 days, secreted the peculiar gigantic fibril with a diameter of 150 μm. The giant fibril is composed of (1→3)-β-glucan chains, i.e., the material of callose tissue.

Keywords: inhibited cell wall formation; macrofibril formation; morphological responses to stress; protoplast of Betula platyphylla; stressed culture condition

About the article

Corresponding author. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan Phone/Fax: +81-(0)92-642-2997

Received: 2011-03-22

Accepted: 2011-09-01

Published Online: 2011-09-30

Published in Print: 2012-03-01

Citation Information: Holzforschung, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/hf.2011.158. Export Citation

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