Jump to ContentJump to Main Navigation
Show Summary Details
More options …
Wood Research and Technology


Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Faix, Oskar

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Salmen, Lennart / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

12 Issues per year

IMPACT FACTOR 2017: 2.079

CiteScore 2017: 1.94

SCImago Journal Rank (SJR) 2017: 0.709
Source Normalized Impact per Paper (SNIP) 2017: 0.979

See all formats and pricing
More options …
Volume 66, Issue 4


Community analysis of preservative-treated southern pine (Pinus spp.) using terminal restriction fragment length polymorphism (T-RFLP) analysis. Part 2: Bacteria field study

Grant T. Kirker / M. Lynn Prewitt / Walter J. Diehl / Susan V. Diehl
Published Online: 2011-12-13 | DOI: https://doi.org/10.1515/hf.2011.172


The effects of wood preservatives on the bacterial community in southern yellow pine were assessed by the molecular method ‘terminal restriction fragment length polymorphism’ (T-RFLP). Stakes, treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1% and 0.25% chlorothalonil (CTN), 0.1% and 0.25% CTN with 2% butylated hydroxytoluene (BHT), and 2% BHT were installed with untreated controls in two field test sites in Mississippi and sampled every 90 days. Bacterial DNA was amplified by means of general 16S rDNA primers. Community data were analyzed to determine the effects of test site, exposure (above or below ground), treatment concentrations, and exposure time on the bacterial communities inhabiting the field stakes. Wood preservatives altered the bacterial communities, which fluctuated in numbers and composition over time. Initial exposure to CTN changed the pattern of species that colonized the stakes, and the bacterial communities did not become more similar to controls after CTN depletion. Bacterial communities on untreated controls were the most similar to each other and changed the least over time. Preservative treatment led to greater population turnover and increased diversity by creating a more unstable bacterial environment, which prevented these communities from reaching equilibrium. Although preservative treatment led to changes over time, there were still many shared species within and between the untreated controls and the different preservative treatments.

Keywords: ammoniacal copper quat (ACQ-C); bacteria community ecology; butylated hydroxytoluene (BHT); chlorothalonil (CTN); T-RFLP; wood colonization; wood decay

About the article

Corresponding author. Department of Forest Products, P.O. Box 9820, Mississippi State, MS 39759, USA

Received: 2011-05-13

Accepted: 2011-10-19

Published Online: 2011-12-13

Published in Print: 2012-05-01

Citation Information: Holzforschung, Volume 66, Issue 4, Pages 529–535, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/hf.2011.172.

Export Citation

Comments (0)

Please log in or register to comment.
Log in