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Wood Research and Technology

Holzforschung

Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Salmén, Lennart

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi


IMPACT FACTOR 2018: 2.579

CiteScore 2018: 2.43

SCImago Journal Rank (SJR) 2018: 0.829
Source Normalized Impact per Paper (SNIP) 2018: 1.082

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1437-434X
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Volume 68, Issue 8

Issues

Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I: Microscopic investigation by POM, UV microscopy, and TOF-SIMS

Peiming Zheng / Dan Aoki / Masato Yoshida / Yasuyuki Matsushita / Takanori Imai / Kazuhiko Fukushima
Published Online: 2014-03-19 | DOI: https://doi.org/10.1515/hf-2013-0231

Abstract

The lignification process from sapwood (sW) to heartwood (hW) in ray parenchyma cells (Pray) of Pinus densiflora has been analyzed by means of ultraviolet (UV) microscopy, acetyl bromide (CH3COBr) lignin determination, and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The cell wall layers were localized by polarized optical microscopy (POM). POM revealed that Pray have almost no secondary wall in sW and have only the outer layer of secondary wall (S1) in the transition zone (TZ) and hW. UV microscopic observations indicated that the secondary wall of Pray, which is in contact with ray tracheids (Trray), begins to lignify in sW, while the secondary wall of Pray, which is not in contact with Trray, is partially lignified in the TZ. The secondary wall of both types of Pray is completely lignified in hW. The CH3COBr lignin content in sW is slightly lower than that in hW. In the TOF-SIMS measurements, the relative intensities of the secondary ions of guaiacyl-lignin (G-lignin) in the rays in sW are significantly lower than those in hW.

Keywords: heartwood; lignification; Pinus densiflora; polarized optical microscopy (POM); ray parenchyma cells; sapwood; time-of-flight secondary ion mass spectrometry (TOF-SIMS); ultraviolet (UV) microscopy

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About the article

Corresponding author: Kazuhiko Fukushima, Laboratory of Wood Chemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan, e-mail:


Received: 2013-11-29

Accepted: 2014-02-14

Published Online: 2014-03-19

Published in Print: 2014-12-01


Citation Information: Holzforschung, Volume 68, Issue 8, Pages 897–905, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/hf-2013-0231.

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