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Wood Research and Technology


Cellulose – Hemicelluloses – Lignin – Wood Extractives

Editor-in-Chief: Salmén, Lennart

Editorial Board: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Burgert, Ingo / Rio, Jose C. / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Koch, Gerald / Lachenal, Dominique / Laine, Christiane / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Potthast, Antje / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Takano, Toshiyuki / Tamminen, Tarja / Theliander, Hans / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi

IMPACT FACTOR 2017: 2.079

CiteScore 2017: 1.94

SCImago Journal Rank (SJR) 2017: 0.709
Source Normalized Impact per Paper (SNIP) 2017: 0.979

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Volume 72, Issue 8


A matrix-resistant HPTLC method to quantify monosaccharides in wood-based lignocellulose biorefinery streams

Josua Timotheus Oberlerchner
  • Department of Chemistry, Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Vienna Konrad Lorenz Straße 24 A-3430, Tulln, Austria
  • Other articles by this author:
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/ Stefan Böhmdorfer
  • Department of Chemistry, Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Vienna Konrad Lorenz Straße 24 A-3430, Tulln, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Thomas Rosenau
  • Department of Chemistry, Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Vienna Konrad Lorenz Straße 24 A-3430, Tulln, Austria
  • Johan Gadolin Process Chemistry Centre, Åbo Akademi University, Porthansgatan 3, Åbo/Turku FI-20500, Finland
  • Other articles by this author:
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/ Antje Potthast
  • Corresponding author
  • Department of Chemistry, Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Vienna Konrad Lorenz Straße 24, A-3430 Tulln, Austria, Phone: +43-1-47654-77412, Fax: +43-1-47654-77059
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Published Online: 2018-04-25 | DOI: https://doi.org/10.1515/hf-2017-0170


A new and robust high performance thin-layer chromatography (HPTLC) method has been developed for carbohydrate analysis in biorefinery streams. The samples can be applied directly from industrial streams without any purification or pretreatment, saving time and resources and avoiding potential alterations to the samples’ composition. The main neutral monosaccharides found in wood-based lignocellulosic biomass – glucose, xylose, mannose, arabinose and galactose – can be separated and quantified using acetonitrile:1-pentanol:water (4:1:1, v/v/v) on silica impregnated with a phosphate buffer. Common minor compounds – glucuronic acid, galacturonic acid, rhamnose, cellobiose and hydroxymethylfurfural (HMF) – can also be detected qualitatively. Matrix compounds, which are a major part of biomass, do not interfere with the analysis. Validation showed that the method is selective, has good repeatability and has limits of detection and quantification in the nanogram range. The visual fingerprint offers information on the samples’ constituents in the case of unknown samples. Also, incomplete hydrolysis is indicated by the visibility of oligosaccharides.

Keywords: biorefinery; carbohydrates; high performance thin layer chromatography; hydrolysis; matrix effects; sample preparation


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About the article

Received: 2017-10-24

Accepted: 2018-03-19

Published Online: 2018-04-25

Published in Print: 2018-07-26

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Citation Information: Holzforschung, Volume 72, Issue 8, Pages 645–652, ISSN (Online) 1437-434X, ISSN (Print) 0018-3830, DOI: https://doi.org/10.1515/hf-2017-0170.

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