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Hormone Molecular Biology and Clinical Investigation

Editor-in-Chief: Chetrite, Gérard S.

Editorial Board: Alexis, Michael N. / Baniahmad, Aria / Beato, Miguel / Bouillon, Roger / Brodie, Angela / Carruba, Giuseppe / Chen, Shiuan / Cidlowski, John A. / Clarke, Robert / Coelingh Bennink, Herjan J.T. / Darbre, Philippa D. / Drouin, Jacques / Dufau, Maria L. / Edwards, Dean P. / Falany, Charles N. / Fernandez-Perez, Leandro / Ferroud, Clotilde / Feve, Bruno / Flores-Morales, Amilcar / Foster, Michelle T. / Garcia-Segura, Luis M. / Gastaldelli, Amalia / Gee, Julia M.W. / Genazzani, Andrea R. / Greene, Geoffrey L. / Groner, Bernd / Hampl, Richard / Hilakivi-Clarke, Leena / Hubalek, Michael / Iwase, Hirotaka / Jordan, V. Craig / Klocker, Helmut / Kloet, Ronald / Labrie, Fernand / Mendelson, Carole R. / Mück, Alfred O. / Nicola, Alejandro F. / O'Malley, Bert W. / Raynaud, Jean-Pierre / Ruan, Xiangyan / Russo, Jose / Saad, Farid / Sanchez, Edwin R. / Schally, Andrew V. / Schillaci, Roxana / Schindler, Adolf E. / Söderqvist, Gunnar / Speirs, Valerie / Stanczyk, Frank Z. / Starka, Luboslav / Sutter, Thomas R. / Tresguerres, Jesús A. / Wahli, Walter / Wildt, Ludwig / Yang, Kaiping / Yu, Qi


CiteScore 2017: 2.48

SCImago Journal Rank (SJR) 2017: 1.021
Source Normalized Impact per Paper (SNIP) 2017: 0.830

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1868-1891
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Volume 16, Issue 3

Issues

Ovariectomy lowers urine levels of unconjugated (+)-catechin, (–)-epicatechin, and their methylated metabolites in rats fed grape seed extract

John K. Cutts
  • Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
  • Other articles by this author:
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/ Thomas R. Peavy
  • Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
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/ Doyle R. Moore
  • Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
  • Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
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/ Jeevan Prasain
  • Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
  • Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Stephen Barnes
  • Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
  • Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
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  • De Gruyter OnlineGoogle Scholar
/ Helen Kim
  • Corresponding author
  • Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
  • Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-11-26 | DOI: https://doi.org/10.1515/hmbci-2013-0044

Abstract

Steroid hormones modulate expression of enzymes that metabolize xenobiotics, including dietary supplements. Half of the human population undergoes menopause, yet the effect of this age-related loss of ovarian steroid hormones on the metabolism of dietary supplements has yet to be determined. Grape seed extract (GSE) is a dietary supplement comprised of monomeric and oligomeric catechins and has health benefits in models of age-related diseases. We hypothesized that surgically-induced loss of ovarian hormones would increase methylation, glucuronidation, and/or sulfation of the grape seed polyphenols (+)-catechin and (–)-epicatechin. Fourteen-week-old spontaneously hypertensive rats (SHRs) were ovariectomized (OVX) or sham-OVX. At 17 weeks of age, SHRs were gavaged with vehicle (water) or GSE (300 mg/kg body weight) once daily for 6 days. Urinary excretion of (+)-catechin, (–)-epicatechin, and their metabolites was analyzed by liquid chromatography-mass spectrometry. Although total urinary output of (+)-catechin, (–)-epicatechin, and their methylated metabolites was unaffected by OVX, the amounts of (+)-catechin, (–)-epicatechin and their methylated metabolites that were not conjugated with glucuronic acid or sulfate were lowered by OVX. Specifically, urine from OVX SHRs administered GSE contained 30% higher proportions (91.8% vs. 62.3%) of glucuronidated (+)-catechin and (–)-epicatechin and glucuronidated methyl (+)-catechin and methyl (–)-epicatechin than urine from sham-OVX SHRs. However, there were no differences in urinary levels of total methylated or sulfated catechins in OVX SHRs. This is the first quantitative characterization of metabolites of grape seed polyphenols in a model of menopause; it indicates that ovariectomy causes either an increase in expression and/or activity of select uridine 5′-diphospho-glucuronosyltransferase(s).

Keywords: catechin; glucuronides; grape seed extract; menopause; metabolism

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About the article

Corresponding author: Helen Kim, University of Alabama at Birmingham, Department of Pharmacology and Toxicology, McCallum Building, Room 460, 1918 University Blvd., Birmingham, AL 35294, USA, Phone: (205) 934-3880, Fax: (205) 934-6944, E-mail: ; and Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA


Received: 2013-08-16

Accepted: 2013-10-29

Published Online: 2013-11-26

Published in Print: 2013-12-01


Citation Information: Hormone Molecular Biology and Clinical Investigation, Volume 16, Issue 3, Pages 129–138, ISSN (Online) 1868-1891, ISSN (Print) 1868-1883, DOI: https://doi.org/10.1515/hmbci-2013-0044.

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