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Hormone Molecular Biology and Clinical Investigation

Editor-in-Chief: Chetrite, Gérard S.

Editorial Board: Alexis, Michael N. / Baniahmad, Aria / Beato, Miguel / Bouillon, Roger / Brodie, Angela / Carruba, Giuseppe / Chen, Shiuan / Cidlowski, John A. / Clarke, Robert / Coelingh Bennink, Herjan J.T. / Darbre, Philippa D. / Drouin, Jacques / Dufau, Maria L. / Edwards, Dean P. / Falany, Charles N. / Fernandez-Perez, Leandro / Ferroud, Clotilde / Feve, Bruno / Flores-Morales, Amilcar / Foster, Michelle T. / Garcia-Segura, Luis M. / Gastaldelli, Amalia / Gee, Julia M.W. / Genazzani, Andrea R. / Greene, Geoffrey L. / Groner, Bernd / Hampl, Richard / Hilakivi-Clarke, Leena / Hubalek, Michael / Iwase, Hirotaka / Jordan, V. Craig / Klocker, Helmut / Kloet, Ronald / Labrie, Fernand / Mendelson, Carole R. / Mück, Alfred O. / Nicola, Alejandro F. / O'Malley, Bert W. / Raynaud, Jean-Pierre / Ruan, Xiangyan / Russo, Jose / Saad, Farid / Sanchez, Edwin R. / Schally, Andrew V. / Schillaci, Roxana / Schindler, Adolf E. / Söderqvist, Gunnar / Speirs, Valerie / Stanczyk, Frank Z. / Starka, Luboslav / Sutter, Thomas R. / Tresguerres, Jesús A. / Wahli, Walter / Wildt, Ludwig / Yang, Kaiping / Yu, Qi

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Volume 26, Issue 2


Ingestion of a natural mineral-rich water in an animal model of metabolic syndrome: effects in insulin signalling and endoplasmic reticulum stress

Cidália D. Pereira
  • Corresponding author
  • Escola Superior de Saúde, Instituto Politécnico de Leiria, Campus 2 – Morro de Lena – Alto do Vieiro, Apartado 4137, 2411-901 Leiria, Portugal
  • Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, Portugal
  • Escola Superior de Saúde, Instituto Politécnico de Leiria, Leiria, Portugal
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Emanuel Passos
  • Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, Portugal
  • CIAFEL – Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Milton Severo
  • Faculty of Medicine, Department of Clinical Epidemiology, Predictive Medicine and Public Health, University of Porto, Porto, Portugal
  • Unidade de Investigação em Epidemiologia (EPIUnit), Instituto de Saúde Pública, University of Porto, Porto, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Isabel Vitó / Xiaogang Wen
  • Escola Superior de Saúde, Instituto Politécnico de Leiria, Campus 2 – Morro de Lena – Alto do Vieiro, Apartado 4137, 2411-901 Leiria, Portugal
  • IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
  • Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
  • Centro Hospitalar Vila Nova de Gaia/Espinho, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Fátima Carneiro
  • Escola Superior de Saúde, Instituto Politécnico de Leiria, Campus 2 – Morro de Lena – Alto do Vieiro, Apartado 4137, 2411-901 Leiria, Portugal
  • IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
  • Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
  • Centro Hospitalar de São João/Medical Faculty of the University of Porto, Porto, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Pedro Gomes
  • Escola Superior de Saúde, Instituto Politécnico de Leiria, Campus 2 – Morro de Lena – Alto do Vieiro, Apartado 4137, 2411-901 Leiria, Portugal
  • Faculty of Medicine, Department of Pharmacology and Therapeutics, University of Porto, Porto, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rosário Monteiro
  • Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, Portugal
  • Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maria J. MartinsORCID iD: http://orcid.org/0000-0002-3560-3261
Published Online: 2016-01-07 | DOI: https://doi.org/10.1515/hmbci-2015-0033


Background: High-fructose and/or low-mineral diets are relevant in metabolic syndrome (MS) development. Insulin resistance (IR) represents a central mechanism in MS development. Glucocorticoid signalling dysfunction and endoplasmic reticulum (ER) and oxidative stresses strongly contribute to IR and associate with MS. We have described that natural mineral-rich water ingestion delays fructose-induced MS development, modulates fructose effects on the redox state and glucocorticoid signalling and increases sirtuin 1 expression. Here, we investigated mineral-rich water ingestion effects on insulin signalling and ER homeostasis of fructose-fed rats.

Materials and methods: Adult male Sprague-Dawley rats had free access to standard-chow diet and different drinking solutions (8 weeks): tap water (CONT), 10%-fructose/tap water (FRUCT) or 10%-fructose/mineral-rich water (FRUCTMIN). Hepatic and adipose (visceral, VAT) insulin signalling and hepatic ER homeostasis (Western blot or PCR) as well as hepatic lipid accumulation were evaluated.

Results: Hepatic p-IRS1Ser307/IRS1 (tendency), p-IRS1Ser307, total JNK and (activated IRE1α)/(activated JNK) decreased with fructose ingestion, while p-JNK tended to increase; mineral-rich water ingestion, totally or partially, reverted all these effects. Total PERK, p-eIF2α (tendency) and total IRS1 (tendency) decreased in both fructose-fed groups. p-ERK/ERK and total IRE1α increasing tendencies in FRUCT became significant in FRUCTMIN (similar pattern for lipid area). Additionally, unspliced-XBP1 increased with mineral-rich water. In VAT, total ERK fructose-induced increase was partially prevented in FRUCTMIN.

Conclusions: Mineral-rich water modulation of fructose-induced effects on insulin signalling and ER homeostasis matches the better metabolic profile previously reported. Increased p-ERK/ERK, adding to decreased IRE1α activation, and increased unspliced-XBP1 and lipid area may protect against oxidative stress and IR development in FRUCTMIN.

Keywords: adipose tissue; endoplasmic reticulum stress; fructose-fed Sprague-Dawley rats; insulin signalling; lipid accumulation; liver; metabolic syndrome; natural mineral-rich water


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About the article

Corresponding author: Cidália D. Pereira, Escola Superior de Saúde, Instituto Politécnico de Leiria, Campus 2 – Morro de Lena – Alto do Vieiro, Apartado 4137, 2411-901 Leiria, Portugal, E-mail: ; Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, Portugal; and Escola Superior de Saúde, Instituto Politécnico de Leiria, Leiria, Portugal

Received: 2015-07-25

Accepted: 2015-11-18

Published Online: 2016-01-07

Published in Print: 2016-05-01

Citation Information: Hormone Molecular Biology and Clinical Investigation, Volume 26, Issue 2, Pages 135–150, ISSN (Online) 1868-1891, ISSN (Print) 1868-1883, DOI: https://doi.org/10.1515/hmbci-2015-0033.

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