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International Journal of Adolescent Medicine and Health

Editor-in-Chief: Merrick, Joav

Editorial Board: Birch, Diana ML / Blum, Robert W. / Greydanus, MD, Dr. HC (Athens), Donald E. / Hardoff, Daniel / Kerr, Mike / Levy, Howard B / Morad, Mohammed / Omar, Hatim A. / de Paul, Joaquin / Rydelius, Per-Anders / Shek, Daniel T.L. / Sher, Leo / Silber, Tomas J. / Towns, Susan / Urkin, Jacob / Verhofstadt-Deneve, Leni / Zeltzer, Lonnie / Tenenbaum, Ariel


CiteScore 2018: 0.79

SCImago Journal Rank (SJR) 2018: 0.350
Source Normalized Impact per Paper (SNIP) 2018: 0.476

Online
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2191-0278
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Volume 30, Issue 1

Issues

Utility of salivary enzyme immunoassays for measuring estradiol and testosterone in adolescents: a pilot study

Mazen Amatoury
  • Corresponding author
  • Academic Department of Adolescent Medicine, The Children’s Hospital at Westmead, Locked Bag 4001, Westmead, New South Wales, 2145, Australia, Phone: +61 2 9845 2178, Fax: +61 2 9845 2517
  • Institute of Endocrinology and Diabetes, The Children’s Hospital at Westmead, Locked Bag 4001, Westmead, New South Wales, 2145, Australia
  • Discipline of Child and Adolescent Health, Sydney Medical School, The University of Sydney, Sydney, New South Wales, 2006, Australia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jennifer W. Lee
  • Institute of Endocrinology and Diabetes, The Children’s Hospital at Westmead, Westmead, New South Wales, Australia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ann M. Maguire
  • Institute of Endocrinology and Diabetes, The Children’s Hospital at Westmead, Westmead, New South Wales, Australia
  • Discipline of Child and Adolescent Health, Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Geoffrey R. Ambler
  • Institute of Endocrinology and Diabetes, The Children’s Hospital at Westmead, Westmead, New South Wales, Australia
  • Discipline of Child and Adolescent Health, Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katharine S. Steinbeck
  • Discipline of Child and Adolescent Health, Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia
  • Academic Department of Adolescent Medicine, The Children’s Hospital at Westmead, Westmead, New South Wales, Australia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-04-09 | DOI: https://doi.org/10.1515/ijamh-2015-0126

Abstract

Aim:

We investigated the utility of enzyme immunoassay kits for measuring low levels of salivary estradiol and testosterone in adolescents and objectively assessed prevalence of blood contamination.

Methods:

Endocrine patients provided plasma and saliva for estradiol (females) or testosterone (males) assay. Saliva samples were also tested with a blood contamination kit.

Results:

Picomolar levels of salivary estradiol in females failed to show any significant correlation with plasma values (r=0.20, p=0.37). The nanomolar levels of salivary testosterone in males showed a strong correlation (r=0.78, p<0.001). A significant number of saliva samples had blood contamination. After exclusion, correlations remained non-significant for estradiol, but strengthened for testosterone (r=0.88, p<0.001).

Conclusion:

The salivary estradiol enzyme immunoassay is not clinically informative at low levels. Users should interpret clinical saliva with caution due to potential blood contamination. Our data supports the utility of the salivary testosterone enzyme immunoassay for monitoring adolescent boys on hormone developmental therapy.

This article offers supplementary material which is provided at the end of the article.

Keywords: adolescent; estradiol; puberty; saliva; testosterone

References

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About the article

Received: 2015-12-22

Accepted: 2016-02-28

Published Online: 2016-04-09


Citation Information: International Journal of Adolescent Medicine and Health, Volume 30, Issue 1, 20150126, ISSN (Online) 2191-0278, DOI: https://doi.org/10.1515/ijamh-2015-0126.

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