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International Journal of Chemical Reactor Engineering

Ed. by de Lasa, Hugo / Xu, Charles Chunbao

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Volume 16, Issue 7

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A Green Process for Synthesis of Geraniol Esters by Immobilized Lipase from Candida Antarctica B Fraction in Non-Aqueous Reaction Media: Optimization and Kinetic Modeling

Ganapati D. Yadav
  • Corresponding author
  • Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, India
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/ Manoj P. Kamble
  • Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, India
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Published Online: 2018-05-30 | DOI: https://doi.org/10.1515/ijcre-2017-0179

Abstract

Enzymatic synthesis of molecules such as flavors, perfumes and fragrances has a great commercial advantage of being marketed as “natural” and also it offers exquisite selectivity of enzymes that can be superior over chemical catalysis. The current work focuses on the enzymatic synthesis of geranyl acetate as model compound, including optimization of reaction conditions such as nature of catalyst, reaction media, speed of agitation, mole ratio and temperature. A variety of esters were also synthesized. Geraniol was esterified with various acids, aromatic esters and vinyl esters in 1:4 molar ratio. Among all vinyl ester was the best giving in good yield (77–100 %) as compared to aromatic esters (5–82 %) and acids (7–31 %). Novozym 435 was found to be most active catalyst with ~96 % conversion and 100 % selectivity in 60 min at 55 °C in n-heptane as solvent for geranyl acetate. The maximum reaction rate was estimated (Vmax = 0.2712 mol L−1 min-1) by using the double reciprocal plot. It is a ternary complex (ordered bi-bi) mechanism with inhibition by geraniol.

This article offers supplementary material which is provided at the end of the article.

Keywords: biotransformation; candida antarctica lipase B; enzyme kinetics; geraniol esters; ternary complex

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About the article

Received: 2017-09-24

Accepted: 2018-05-05

Revised: 2018-01-21

Published Online: 2018-05-30


Competing interests: Both authors declare that they have no conflict of interest and have contributed equally.


Citation Information: International Journal of Chemical Reactor Engineering, Volume 16, Issue 7, 20170179, ISSN (Online) 1542-6580, DOI: https://doi.org/10.1515/ijcre-2017-0179.

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