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International Journal of Food Engineering

Editor-in-Chief: Chen, Xiao Dong

IMPACT FACTOR 2017: 0.923

CiteScore 2018: 1.02

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Volume 15, Issue 10


A Culture-Independent Method for Enumeration of Viable Load of Lactobacillus acidophilus NCFM by using Real-Time PCR

Md. Aslam Ali / Ziwei Zhang / Hongfu Li / Yashuo Zhang / Shiqian Fu / Wei Zhang / Chaoxin Man / Yujun Jiang
Published Online: 2019-10-17 | DOI: https://doi.org/10.1515/ijfe-2019-0079


The study was performed to develop a non-culture method to quantify viable loads of Lactobacillus acidophilus NCFM using RNA-based molecular technique. The ‘growth curve’ and ‘cycle threshold curve’ were developed respectively by plate counting and using cycle threshold (CT) values. ‘Standard curve’ was constructed using cells per milliliter and relative CT values. A maximum viable count (1.5 ± 0.15) × 1010 cells/mL with a minimum CT value 20.18 ± 0.56 was achieved following 18 h of growth. The two parameters were inversely proportional to each other over the exponential growth. The ‘standard curve’ corresponded to equation y = 2E + 28e−2.034x (y = cells/mL, x = CT value; R2 = 0.993), and no sample showed significant difference between ‘plate count’ and relative ‘PCR count’ following the validation process. Industrial adaptation of this method in dairy processing could potentially contribute to a faster enumeration of viable L. acidophilus NCFM compared to plate counting.

Keywords: culture-independent method, L. acidophilus NCFM; CT (cycle threshold); PCR (polymerase chain reaction); enumeration


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About the article

Received: 2019-03-05

Accepted: 2019-09-15

Revised: 2019-07-03

Published Online: 2019-10-17

Conflicts of interest: The authors declare that they have no conflict of interest.

Citation Information: International Journal of Food Engineering, Volume 15, Issue 10, 20190079, ISSN (Online) 1556-3758, DOI: https://doi.org/10.1515/ijfe-2019-0079.

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