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Interdisciplinary Toxicology

The Journal of Institute of Experimental Pharmacology of Slovak Academy of Sciences

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1337-9569
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Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats

Preeti Yadav
  • Division of Pharmacology & Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior, India
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/ Sunil Jadhav
  • Division of Pharmacology & Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior, India
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/ Vinesh Kumar
  • Division of Pharmacology & Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior, India
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/ Kirtee Kaul / Satish Pant
  • Division of Pharmacology & Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior, India
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/ Swaran Flora
  • Division of Pharmacology & Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior, India
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Published Online: 2012-05-11 | DOI: https://doi.org/10.2478/v10102-012-0001-x

Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats

The effect of 2- pyridine aldoxime methyl chloride (2-PAMCl) and atropine with or without curcumin was investigated in dichlorvos (2,2-dichlorovinyl dimethyl phosphate; DDVP) induced toxicity in rats. Rats were exposed to DDVP (2 mg/kg sub-cutaneously) once daily for the period of 21 days. Post DDVP exposure, rats were further treated with 2-PAMCl (50 mg/kg intramuscular, once daily) + atropine (10 mg/kg, i.m. once daily) with or without curcumin (200 mg/kg; oral; once daily) for further 21 days. We observed a significant increase in lipid peroxidation (LPO), reactive oxygen species (ROS), oxidized glutathione (GSSG), while there was a significant decrease in antioxidant enzymes, brain acetylcholinesterase (AChE) and 5-hydroxy tryptamine (5-HT) activity on DDVP exposure of rats. These alterations were restored significantly by co-administration of 2-PAMCl + atropine in DDVP exposed rats. Curcumin when co-supplemented with 2-PAMCl + atropine also significantly protected serum aspartate aminotransferase (AST) and restored brain AChE activity and 5-HT level in animals sub-chronically exposed to DDVP. Histopathological observations along with biochemical changes in rat blood and tissues revealed significant protection offered by 2-PAMCl + atropine against DDVP. The results indicate that DDVP-induced toxicity can be significantly protected by co-administration of 2-PAMCl + atropine individually, however, curcumin co-supplementation with 2-PAMCl + atropine provides more pronounced protection, concerning particularly neurological disorders.

Keywords: Dichlorvos toxicity; 2-PAM. Cl; curcumin; atropine; oxidative stress

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About the article


Published Online: 2012-05-11

Published in Print: 2012-03-01


Citation Information: Interdisciplinary Toxicology, Volume 5, Issue 1, Pages 1–8, ISSN (Online) 1337-9569, ISSN (Print) 1337-6853, DOI: https://doi.org/10.2478/v10102-012-0001-x.

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