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Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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2299-4831
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Use of Thymol Formulations, Amitraz, and Oxalic Acid for the Control of the Varroa Mite in Honey Bee (Apis mellifera carnica) Colonies

Aleš Gregorc
  • Agricultural Institute of Slovenia, Hacquetova 17, SI-1000 Ljubljana
  • University of Maribor, Faculty of Agriculture and Life Sciences, Slovenia
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/ Ivo Planinc
  • Veterinary Faculty of the University of Ljubljana, National Veterinary Institute, Ljubljana, Slovenia
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Published Online: 2013-01-12 | DOI: https://doi.org/10.2478/v10289-012-0024-8

Abstract

Experiments were conducted in three apiaries to assess the comparative efficacy of: Thymovar (Andermatt BioVet AG); Apiguard (Vita Europe Ltd., UK); an oxalic acid solution (OA) which consisted of 2.9% oxalic acid and 31.9% sugar in water; and amitraz fumigation, for controlling the honey bee mite Varroa destructor. Mite mortality increased significantly (p<0.001) in the Thymovar, Apiguard, OA or amitraz treated colonies. The relative mite mortality after: four OA applications, two Thymovar or two Apiguard applications during August and September in the Senično apiary was 41.80% (±14.31), 14.35% (±10.71), and 18.93% (±13.56), respectively. In the control, i.e. untreated colonies, the mite natural mortality was reduced by 3.10% (±3.50). In the Bohinj apiary, two Apiguard applications and a single amitraz treatment resulted in reducing the mite populations by 19.71% (±12.61) and 23.89% (±14.25), respectively. At the Mediterranean located apiary of Vipava, the Thymovar and Apiguard treatments trigged 59.02% (±17.28) and 46.50% (±13.33) of the total mite reduction. In the Vipava apiary, colonies treated with any miticide during the brood period presented no difference (P>0.05) in efficacy. The results indicate that OA, Thymovar, Apiguard or amitraz fumigations are of limited use during the brood periods.

Streszczenie

Celem pracy było porównanie skuteczności zwalczania roztoczy Varroa destructor za pomocą tymolu, amitrazy oraz kwasu szczawiowego w rodzinach pszczelich z czerwiem. Badania wykonano w pasiekach zlokalizowanych w miejscowości Senično i Bohinj (położonych w strefie klimatu kontynentalnego) oraz w miejscowości Vipava (położonej w strefie klimatu śródziemnomorskiego) na Słowenii. Do zwalczania pasożyta zastosowano Thymovar (Andermatt BioVet AG); Apiguard (Vita Europe Ltd., UK); 12,5% roztwór amitrazy w formie odymiania oraz wodny roztwór kwasu szczawiowego, zawierający 2,9% kwasu szczawiowego i 31,9% cukru. Po wykonaniu zabiegów leczniczych, we wszystkich rodzinach stwierdzono istotny wzrost śmiertelności roztoczy (p<0,001). W pasiece Senično, po czterokrotnej aplikacji kwasu szczawiowego (grupa a), dwukrotnej aplikacji Thymovaru (grupa b) oraz po dwukrotnym podaniu Apiguardu (grupa c) w okresie od sierpnia do września, względna śmiertelność roztoczy wynosiła odpowiednio 41,8% (±14,31); 14,35% (±10,71) i 18,93% (±13,56). W grupie kontrolnej, na skutek naturalnej śmiertelności roztoczy V. destructor, liczba pasożytów zmniejszyła się w tym okresie o 3,1% (±3,5). W Bohinj, w rodzinach, którym dwukrotnie zaaplikowano Apiguard, a następnie wykonano jeden zabieg odymiania amitrazą, populacja roztoczy uległa redukcji odpowiednio o 19,71% (±12,61) i 23,89% (±14,25). Dwukrotne zastosowanie Thymovaru (grupa a) i Apiguardu (grupa b) w pasiece zlokalizowanej w strefie śródziemnomorskiej (Vipava) spowodowało obniżenie liczebności pasożytów w rodzinach o 59,02% (±17,28) i 46,50% (±13,33). Wyniki badań wskazują na ograniczone zastosowanie kwasu szczawiowego, Thymovaru, Apiguardu i amitrazy przy zwalczaniu roztoczy Varroa w okresie, w którym w rodzinach pszczelich obecny jest czerw.

Keywords : Varroa destructor; mite control; Apiguard; Thymovar; oxalic acid; amitraz.

Słowa kluczowe: Varroa destructor; zwalczanie; Apiguard; Thymovar; kwas szczawiowy; amitraza.

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About the article

Published Online: 2013-01-12

Published in Print: 2012-12-01


Citation Information: Journal of Apicultural Science, ISSN (Print) 2299-4831, DOI: https://doi.org/10.2478/v10289-012-0024-8.

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