Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

2 Issues per year


IMPACT FACTOR 2016: 0.722
5-year IMPACT FACTOR: 0.944

CiteScore 2016: 0.84

SCImago Journal Rank (SJR) 2016: 0.414
Source Normalized Impact per Paper (SNIP) 2016: 0.616

Open Access
Online
ISSN
2299-4831
See all formats and pricing
More options …

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Ghasem Haghi
  • Corresponding author
  • Phytochemistry Group, Jundi Shapour Medicinal Plants Research Center, 87135-1178 Kashan, Iran
  • Barij Essence Pharmaceutical Company Research Center, Kashann, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alireza Hatami
  • Phytochemistry Group, Jundi Shapour Medicinal Plants Research Center, 87135-1178 Kashan, Iran
  • Barij Essence Pharmaceutical Company Research Center, Kashann, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mehdi Mehran
Published Online: 2013-12-31 | DOI: https://doi.org/10.2478/jas-2013-0015

Abstract

A reverse-phase high-performance liquid chromatographic (RP -HPLC ) method was developed and validated for the analysis of honeybee venom samples and drug products containing honeybee venom. The validation parameters were linearity, sensitivity, precision, and recovery. Melittin is the main component of honeybee venom was extracted with pure water, and then evaluated by RP -HPLC with a photodiode array (PDA ) detector. Separation of the samples was achieved on a Europa Protein C18 column with linear gradient elution of acetonitrile and 0.4% phosphoric acid at 25°C. There was a flow rate of 1 mL/min. Detection was set at 220 nm. Limits of detection (LOD ) and quantification (LO Q) for melittin were 1.1 and 3.2 μg/mL, respectively. The amount of melittin in honeybee venom samples ranged from 21.9 to 66.4 %.

Keywords: honeybee venom; melittin; method validation; RP -HPLC

  • Gauldie J., Hanson J. M., Shipolini R. A., Vernon C. A. (1978) The Structures of some peptides from bee venom. European Journal of Biochemistry 83: 405-410. DOI: 10.1111/j.1432-1033.1978.tb12106.x Gauldie J., Hanson J. M., Rumjanek F. D., Shipolini R.CrossrefGoogle Scholar

  • A., Vernon C. A. (1976) The peptide components of bee venom. European Journal of Biochemistry 61: 369-376. DOI: 10.1111/j.1432-1033.1976. tb10030.x Habermann E. (1972) Bee and wasp venoms. Science 177: 314-322. DOI: 10.1126/science. 177.4046.314CrossrefGoogle Scholar

  • Kim H. W., Kwon Y. B., Ham T. W., Roh D. H., Yoon S. Y., Lee H. J., Han H. J., Yang I. S., Beitz A. J., Lee J. H. (2003) Acupoint stimulation using bee venom attenuates formalin induced pain behavior and spinal cord fos expression in rats. Journal of Veterinary Medical Science 65: 349-355. DOI: 10.1292/jvms.65.349CrossrefGoogle Scholar

  • Kokot Z. J., Matysiak J. (2009) Simultaneous determination of major constituents of honeybee venom by LC-DAD. Chromatographia 69: 1401-1405. DOI: 10.1365/s10337-009-1052-9CrossrefGoogle Scholar

  • Kwon Y. B., Lee H. J., Han H. J., Mar W. C., Kang S. K., Yoon O. B., Beitz A. J., Lee J. H. (2002) The water-soluble fraction of bee venom produces antinociceptive and anti-inflammatory effects on rheumatoid arthritis in rats. Life Sciences 71: 191-204. DOI: 10.1016/S0024-3205(02)01617-X Kwon Y. B., Ham T. W., Kim H. W., Roh D. H., Yoon S. Y., Han H. J, Yang I. S., Kim K. W., Beitz A. J., Lee J. H. (2005) Water soluble fraction (<10 kDa) from bee venom reduces visceral pain behavior through spinal alpha 2-adrenergic activity in mice. Pharmacology Biochemistry and Behavior 80: 181-187. DOI: 10.1016/j.pbb.2004.10.017CrossrefGoogle Scholar

  • Lee M. S., Pittler M. H., Shin B. C., Kong J. C., Ernst E. (2008) Bee venom acupuncture for musculoskeletal pain: A review. The Journal of Pain 9: 289-297. DOI: 10.1016/j.jpain.2007.11.012CrossrefGoogle Scholar

  • Matysiak J., Schmelzer C. E. H., Neubert R. H. H., Kokot Z. J. (2011) Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 54: 273-278. DOI: 10.1016/j. jpba.2010.08.020PubMedWeb of ScienceCrossrefGoogle Scholar

  • Pacáková V., Stulik K. (2000) Validation of a method for determination of phospholipase A2 and melittin in bee venom preparations by capillary electrophoresis. Journal of AOAC International 83: 549-554.Google Scholar

  • Pacáková V., Štulík K., Hau P. T., Jelínek I., Vinš I., Sýkora D. (1995) Comparison of high-performance liquid chromatography and capillary electrophoresis for the determination of some bee venom compounds. Journal of Chromatography A 700: 187-193. DOI: 10.1016/0021-9673(94)01170-j Park H. J., Lee S. H., Son D. J., Oh K. W., Kim K. H., Song H. S., Kim G. J., Oh G. T., Yoon D. Y., Hong J. T. (2004) Antiarthritic effect of bee venom: inhibition of inflammation mediator generation by suppression of NF-κB through interaction with the p50 subunit. Arthritis and Rheumatism 50: 3504-3515. DOI: 10.1002/art.20626CrossrefGoogle Scholar

  • Putz T., Ramoner R., Gander H., Rahm A., Bartsch G., Thurnher M. (2006) Antitumor action and immune activation through cooperation of bee venom secretory phospholipase A2 and phosphatidylinositol-( 3,4)-bisphosphate. Cancer Immunology, Immunotherapy 55: 1374-1383. DOI: 10.1007/ s00262-006-0143-9CrossrefGoogle Scholar

  • Rader K., Wildfeuer A., Wintersberger F., Bossinger P., Mucke H. W. (1987) Characterization of bee venom and its main components by high performance liquid chromatography. Journal of Chromatography 408: 341-348. DOI: 10.1016/s0021-9673(01)81820-3CrossrefGoogle Scholar

  • Russell P. J., Hewish D., Carter T., Sterling-Levis K., Ow K., Hattarki M., Doughty L., Guthrie R., Shapira D., Molloy P. L., Werkmeister J. A., Kortt A. A. (2004) Cytotoxic properties of immunoconjugates containing melittin-like peptide 101 against prostate cancer: in vitro and in vivo studies. Cancer Immunology,Immunotherapy 53: 411-421. DOI: 10.1007/ s00262-003-0457-9Google Scholar

  • Rybak M., Skubida P. (2007) Application of coupled electrical and sound stimulation for honeybee venom collection. Journal of Apicultural Science 51: 63-66Google Scholar

  • Rybak-Chmielewska H., Szczęsna T. (2004) HPLC study of chemical composition of honeybee (Apis mellifera L.) venom. Journal of Apicultural Science 48: 103-109.Google Scholar

  • Rybak M., Muszyńska J., Skubida P. (1995) A technology for bee venom collection. Pszczelnicze Zeszyty Naukowe 39(2): 223-231.Google Scholar

  • Son D. J., Lee J. W., Lee Y. H., Song H. S., Lee C. K., Hong J. T. (2007) Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds. Clinical Pharmacology and Therapeutics 115: 246-270. DOI: 10.1016/j.pharmthera.2007.04.004Web of ScienceCrossrefGoogle Scholar

  • Szokan G., Harvath J., Almas M., Saftics G., Palocz A. (1994) Liquid chromatographic analysis and separation of polypeptide components from honeybee venoms. Journal of Liquid Chromatography and Related Technologies 17: 3333-3349. DOI: 10.1080/10826079408013516CrossrefGoogle Scholar

  • Zhou J., Zhao J., Zhang S., Shen J., Qi Y., Xue X., Li Y., Wub L., Zhang J., Chen F., Chen L. (2010) Quantification of melittin and apamin in bee venom lyophilized powder from Apis mellifera by liquid chromatography- diode array detector-tandem mass spectrometry. Analytical Biochemistry 404: 171-178. DOI: 10.1016/j.ab.2010.05.014CrossrefGoogle Scholar

  • Ziyavitdinov Z. F., Inogamov U. K., Sagdiev N. Z. (1995) Development of a method for the complex isolation of physiologically active components from bee venom. Chemistry of Natural Compounds 31: 726-730. DOI: 10.1007/bf01386189 CrossrefGoogle Scholar

About the article

Published Online: 2013-12-31

Published in Print: 2013-12-01


Citation Information: Journal of Apicultural Science, ISSN (Print) 2299-4831, DOI: https://doi.org/10.2478/jas-2013-0015.

Export Citation

This content is open access.

Comments (0)

Please log in or register to comment.
Log in