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Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Ghasem Haghi
  • Corresponding author
  • Phytochemistry Group, Jundi Shapour Medicinal Plants Research Center, 87135-1178 Kashan, Iran
  • Barij Essence Pharmaceutical Company Research Center, Kashann, Iran
  • Email:
/ Alireza Hatami
  • Phytochemistry Group, Jundi Shapour Medicinal Plants Research Center, 87135-1178 Kashan, Iran
  • Barij Essence Pharmaceutical Company Research Center, Kashann, Iran
/ Mehdi Mehran
  • Barij Essence Pharmaceutical Company Research Center, Kashann, Iran
Published Online: 2013-12-31 | DOI: https://doi.org/10.2478/jas-2013-0015


A reverse-phase high-performance liquid chromatographic (RP -HPLC ) method was developed and validated for the analysis of honeybee venom samples and drug products containing honeybee venom. The validation parameters were linearity, sensitivity, precision, and recovery. Melittin is the main component of honeybee venom was extracted with pure water, and then evaluated by RP -HPLC with a photodiode array (PDA ) detector. Separation of the samples was achieved on a Europa Protein C18 column with linear gradient elution of acetonitrile and 0.4% phosphoric acid at 25°C. There was a flow rate of 1 mL/min. Detection was set at 220 nm. Limits of detection (LOD ) and quantification (LO Q) for melittin were 1.1 and 3.2 μg/mL, respectively. The amount of melittin in honeybee venom samples ranged from 21.9 to 66.4 %.

Keywords: honeybee venom; melittin; method validation; RP -HPLC

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About the article

Published Online: 2013-12-31

Published in Print: 2013-12-01

Citation Information: Journal of Apicultural Science, ISSN (Print) 2299-4831, DOI: https://doi.org/10.2478/jas-2013-0015. Export Citation

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