Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

2 Issues per year


IMPACT FACTOR 2016: 0.722
5-year IMPACT FACTOR: 0.944

CiteScore 2016: 0.84

SCImago Journal Rank (SJR) 2015: 0.392
Source Normalized Impact per Paper (SNIP) 2015: 0.470

Open Access
Online
ISSN
2299-4831
See all formats and pricing
In This Section

Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Renata Markiewicz-Żukowska
  • Department of Bromatology, Medical University of Białystok, Poland
/ Sylwia K. Naliwajko
  • Department of Bromatology, Medical University of Białystok, Poland
/ Emilia Bartosiuk
  • Department of Bromatology, Medical University of Białystok, Poland
/ Justyna Moskwa
  • Department of Bromatology, Medical University of Białystok, Poland
/ Valery Isidorov
  • Institute of Chemistry, University of Białystok, Poland
/ Jolanta Soroczyńska
  • Department of Bromatology, Medical University of Białystok, Poland
/ Maria H. Borawska
  • Corresponding author
  • Department of Bromatology, Medical University of Białystok, Poland
  • Email:
Published Online: 2013-12-31 | DOI: https://doi.org/10.2478/jas-2013-0025

Abstract

Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 μg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.

Keywords: antioxidant activity; beebread; chemical composition; cytotoxicity; glioblastoma multiforme

  • Abouda Z., Zerdani I., Kalalou I., Faid M., Ahami M. T. (2011) The antibacterial activity of Moroccan bee bread and bee-pollen (fresh and dried) against pathogenic bacteria. Research Journal of Microbiology 6(4): 376-384. DOI: 10.3923/jm.2011.376.384CrossrefGoogle Scholar

  • Agnihotri S., Burrell K. E., Wolf A., Jalali S., Hawkins C., Rutka J. T., Zadeh G. (2013) Glioblastoma, a brief review of history, molecular genetics, animal models and novel therapeutic strategies. Archivum Immunologiae et Therapiae Experimentalis (Warsz.) 61(1): 25-41. DOI: 10.1007/s00005-012-0203-0PubMedWeb of ScienceCrossrefGoogle Scholar

  • Alencar S. M., Oldoni T. L., Castro M. L., Cabral I. S., Costa-Neto C. M., Cury J. A., Rosalen P. L., Ikegaki M. (2007) Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. Journal of Ethnopharmacology 113(2): 278-283. DOI: 10.1016/j.jep.2007.06.005CrossrefPubMedWeb of ScienceGoogle Scholar

  • Aljadi A. M., Kamaruddin M. Y. (2004) Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chemistry 85(4): 513-518. DOI: 10.1016/S0308-8146(02)005CrossrefGoogle Scholar

  • Audisio M. C., Terzolo H. R., Apella M. C. (2005) Bacteriocin from honeybee beebread Enterococcus avium, active against Listeria monocytogenes. Applied and Environmental Microbiology 71(6): 3373-3375. DOI: 10.1128/AEM.71.6.3373-3375PubMedCrossrefGoogle Scholar

  • Baltrušaitytė V., Venskutonis P. R., Čeksterytė V. (2007a) Antibacterial activity of honey and beebread of different origin against S. aureus and S. epidermidis. Food Technology and Biotechnology 45(2): 201-208.Google Scholar

  • Baltrušaitytė V., Venskutonis P. R., Čeksterytė V. (2007b) - Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chemistry 101(2): 502-514. DOI: 10.1016/j. foodchem.2006.02.007CrossrefGoogle Scholar

  • Barbarić M., Mišković K., Bojić M., Lončar M. B., Smolčić-Bubalo A., Debeljak Z., Medić-Šarić M. (2011) Chemical composition of the ethanolic propolis extracts and its effect on HeLa cells. Journal of Ethnopharmacology 135(3): 772-778. DOI: 10.1016/j.jep.2011.04.015PubMedCrossrefWeb of ScienceGoogle Scholar

  • Benatti P., Peluso G., Nicolai R., Calvani M. (2004) Polyunsaturated fatty acids: biochemical, nutritional and epigenetic properties. Journal of the American College of Nutrition 23(4): 281-302. DOI: 10.1080/07315724.2004.10719371CrossrefPubMedGoogle Scholar

  • Borawska M. H., Czechowska S. K., Markiewicz R., Socha K., Nazaruk J., Palka J., Isidorov V. A. (2010) Enhancement of antibacterial effects of extracts from Cirsium species using sodium picolinate and estimation of their toxicity. Natural Product Research 24(6): 554-561. DOI: 10.1080/14786410902770728CrossrefWeb of SciencePubMedGoogle Scholar

  • Borges K. S., Brassesco M. S., Scrideli C. A., Soares A. E., Tone L. G. (2011) Antiproliferative effects of Tubibee propolis in glioblastoma cell lines. Genetics and Molecular Biology 34(2): 310-314. DOI: 10.1590/ S1415-47572011000200024Web of SciencePubMedCrossrefGoogle Scholar

  • Cabral I. S. R., Oldoni T. L. C., Prado A., Bezerra R. M. N., Alencar S. M., Ikegaki M., Rosalen P. L. (2009) Phenolic composition, antibacterial and antioxidant activities of Brazilian red propolis. Quimica Nova 32(6): 1523-1527. DOI: 10.1590/S0100-40422009000600031CrossrefWeb of ScienceGoogle Scholar

  • Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B. (1987) Evaluation of a tetrazoliumbased semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Research 47(4): 936-942.Google Scholar

  • Čeksterytė V., Račys J., Kaškonienė V. Venskutonis P. R. (2008) Fatty acid composition in beebread. Biologija 54(4): 253-257. DOI: 10.2478/v10054-008-0052-2CrossrefGoogle Scholar

  • Cimini A., Cristiano L., Colafarina S., Benedetti E., Di Loreto S., Festuccia C., Amicarelli F., Canuto R. A., Cerù M. P. (2005) PPARgamma-dependent effects of conjugated linoleic acid on the human glioblastoma cell line (ADF). International Journal of Cancer 117(6): 923-33. DOI: 10.1002/ijc.21272CrossrefGoogle Scholar

  • Da Silva Frozza C. O., Garcia C. S., Gambato G., de Souza M. D., Salvador M., Moura S., Padilha F.F., Seixas F. K., Collares T., Borsuk S., Dellagostin O. A., Henriques J. A., Roesch-Ely M. (2012) Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food and Chemical Toxicology 52: 137-142. DOI: 10.1016/j.fct.2012.11.013CrossrefGoogle Scholar

  • Djeridane A., Yousfi M., Nadjemi B., Boutassouna D., Stoker P., Vidal N. (2006) Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 97(4): 654-660. DOI: 10.1016/j.foodchem.2005.04.028CrossrefGoogle Scholar

  • Gangemi R. M., Griffero F., Marubbi D., Perera M., Capra M. C., Malatesta P., Ravetti G. L., Zona G. L., Daga A., Corte G. (2009) SOX2 silencing in glioblastoma tumor- initiating cells causes stop of proliferation and loss of tumorigenicity. Stem Cells 27(1): 40-48. DOI: 10.1634/stemcells.2008-0493CrossrefWeb of ScienceGoogle Scholar

  • Gheldof N., Wang X. H., Engeseth N. J. (2002) Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricultural and Food Chemistry 50(21): 5870-5877. DOI: 10.1021/jf0256135PubMedCrossrefGoogle Scholar

  • Gilliam M. (1979) Microbiology of pollen and bee bread: the yeasts. Apidologie 10(1): 43-53. DOI: 10.1051/apido:19790106CrossrefGoogle Scholar

  • Gómez-Caravaca A. M., Gómez-Romero M., Arráez- Román D., Segura-Carretero A., Fernández-Gutiérrez. A. (2006) Advances in the analysis of phenolic compounds in products derived from bees. Journal of Pharmaceutical and Biomedical Analysis 41(4): 1220-1234. DOI: 10.1016/j.jpba.2006.03.002CrossrefGoogle Scholar

  • Guarini L., Su Z. Z., Zucker S., Lin J., Grunberger D., Fisher P. B. (1992) Growth inhibition and modulation of antigenic phenotype in human melanoma and glioblastomamultiforme cells by caffeic acid phenethyl ester (CAPE). Cell and Molecular Biology 38(5): 513-527.Google Scholar

  • Huang W. J., Huang C. H., Wu C. L., Lin J. K., Chen Y. W., Lin C. L., Chuang S. E., Huang C. Y., Chen C. N. (2007) Propolin G, a prenylflavanone, isolated from Taiwanese propolis, induces caspase-dependent apoptosis in brain cancer cells. Journal of Agricultural and Food Chemistry 55(18): 7366-7376. DOI: 10.1021/ jf0710579CrossrefGoogle Scholar

  • Huang W. J., Lin C. W., Lee C. Y., Chi L. L., Chao Y. C., Wang H. N., Chiou B. L., Chen T. J., Huang C. Y., Chen C. N. (2011) NBM-HD-3, a novel histone deacetylase inhibitor with anticancer activity through modulation of PTEN and AKT in brain cancer cells. Journal of Ethnopharmacology 136(1): 156-167. DOI: 10.1016/j.jep.2011.04.034CrossrefPubMedWeb of ScienceGoogle Scholar

  • Isidorov V. A., Isidorova A. G., Szczepaniak L., Czyżewska U. (2009) Gas chromatographic-mass spectrometric investigation of the chemical composition of beebread. Food Chemistry 115(3): 1056-1063. DOI:10.1016/j.foodchem.2008.12.025CrossrefWeb of ScienceGoogle Scholar

  • Kas’ianenko V. I., Komisarenko I. A., Dubtsova E. A. (2011) Correction of atherogenic dyslipidemia with honey, pollen and bee bread in patients with different body mass. Terapevticheskii Arkhiv 83(8): 58-62.Google Scholar

  • Kaškoniene V., Venskutonis P. R., Ceksteryte V. (2008) Composition of volatile compounds of honey of various floral origin and beebread collected in Lithuania. Food Chemistry 111(4): 988-997. DOI: 10.1016/j.foodchem.2008.05.021Web of ScienceCrossrefGoogle Scholar

  • Leaver H. A., Bell H. S., Rizzo M. T., Ironside J. W., Gregor A., Wharton S. B., Whittle I. R. (2002) Antitumour and pro-apoptotic actions of highly unsaturated fatty acids in glioma. Prostaglandins, Leukotrienes and Essential Fatty Acids 66(1): 19-29. DOI: 10.1054/ plef.2001.0336CrossrefGoogle Scholar

  • Maggiora M., Bologna M., Cerù M. P., Possati L., Angelucci A., Cimini A., Miglietta A., Bozzo F., Margiotta C., Muzio G., Canuto R. A. (2004) An overview of the effect of linoleic and conjugated-linoleic acids on the growth of several human tumor cell lines. International Journal of Cancer 112(6): 909-919. DOI: 10.1002/ijc.20519CrossrefGoogle Scholar

  • Markiewicz-Żukowska R., Car H., Naliwajko S. K., Sawicka D., Szynaka B., Chyczewski L., Isidorov V., Borawska M. H. (2012) Ethanolic extract of propolis, chrysin, CAPE inhibit human astroglia cells. Advancesin Medical Sciences 57(2): 208-216. DOI: 10.2478/ v10039-012-0042-6CrossrefWeb of ScienceGoogle Scholar

  • Morais M., Moreira L., Feas X., Estevinho L. M. (2011) Honeybee-collected pollen from five Portuguese Natural Parks: palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food and Chemical Toxicology 49(5): 1096-1101. DOI: 10.1016/j.fct.2011.01.020CrossrefGoogle Scholar

  • Mutsaers M., van Blitterswijk H., van ‘t Leven L., Kerkvliet J., van de Waerdt J. (2005) Bee bread. In: Mutsaers M. (Ed.), Bee products properties, processing and marketing. Agromisa Foundation. Wageningen. pp. 34-35.Google Scholar

  • Sulaiman G. M., Al Sammarrae K. W., Ad’hiah A. H., Zucchetti M., Frapolli R., Bello E., Erba E., D’Incalci M., Bagnati R. (2011) Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials. Food and Chemical Toxicology 49(9): 2415-2421. DOI: 10.1016/j.fct.2011.06.060CrossrefGoogle Scholar

  • Watanabe M. A., Amarante M. K., Conti B. J., Sforcin J. M. (2011) Cytotoxic constituents of propolis inducing anticancer effects: a review. Journal of Pharmacy and Pharmacology 63(11): 1378-1386. DOI: 10.1111/j.2042-7158.2011.01331.x CrossrefWeb of ScienceGoogle Scholar

About the article

Published Online: 2013-12-31

Published in Print: 2013-12-01


Citation Information: Journal of Apicultural Science, ISSN (Print) 2299-4831, DOI: https://doi.org/10.2478/jas-2013-0025.

Export Citation

This content is open access.

Comments (0)

Please log in or register to comment.
Log in