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Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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Determination of Antioxidant Capacity, Flavonoids, and Total Phenolic Content in Eucalyptus and Clover Honeys

María C. Ciappini
  • Corresponding author
  • CIDTA - Universidad Tehnológica Nacional – Facultad Regional Rosario – E. Zeballos 1341 – S2000BUN Rosario – Argentina
  • Email:
/ Fernando S. Stoppani
  • CIDTA - Universidad Tehnológica Nacional – Facultad Regional Rosario – E. Zeballos 1341 – S2000BUN Rosario – Argentina
Published Online: 2014-05-27 | DOI: https://doi.org/10.2478/jas-2014-0010


Polyphenolic compounds reportedly produce physiological effects that are beneficial to health. Bee products are particularly rich in polyphenolic compounds. We determined the antioxidant capacity and the phenolic and flavonoid compounds content of 81 samples of honey. We used the Folin-Ciocalteu reagent method to evaluate the total phenolic content. The antioxidant activities were evaluated using in vitro scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH ) and hydroxyl radicals (OH ), Trolox equivalent antioxidant capacity (TEAC ), and ferric-reducing antioxidant capacity (FRAC ). Total phenolic content ranged from 40.3 to 193.0 mg gallic acid equivalents (GAE )/100 g; the flavonoid content varied from 1.4 to 7.5 mg quercetin equivalents (QE)/100 g. Eucalyptus honeys exhibited significantly higher phenolic content and free radical-scavenging activity than clover honey samples (p<0.05 for all). Principal component analysis explained 73% of the differences observed in antiradical activity with respect to floral origin. Total phenolic content may be more useful than the radical-scavenging assay for detecting antioxidant capacity in honey; it also represents the variable that most appropriately discriminated among these honeys.

Keywords: DPPH; ferric-reducing antioxidant capacity; flavonoids in honey; hydroxyl radicals; polyphenols; Trolox equivalent antioxidant capacity


  • Aljadi A. M., Kamaruddin M. Y. (2004) Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chemistry 85: 513-518.

  • Arnao M. B., Cano A., Acosta M. (2001) The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry 73: 239-244.

  • Álvarez Suárez J. M., Tulipani S., Romandini S., Bompadre S., Vidal A., Battino S. (2009) Determinación de polifenoles y flavonoides totales en mieles uniflorales de Cuba y de su capacidad antioxidante total. In: Proceedings of III Congreso Cubano de Apicultura. La Habana - Cuba. 9-13 March 2009: 33.

  • Baltrušaitytė V., Venskutonis P. R., Čeksterytė V. (2007) Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chemistry 101: 502-514. DOI: 10.1016/j.foodchem.2006.02.007 [Crossref]

  • Beretta G., Granata P., Ferrero M., Orioli M., Facino R. M. (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta 533: 185-191.

  • Bertonceij J., Dobersek U., Jamnik M., Golob T. (2007) Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chemistry 105(2): 822-8. [Crossref]

  • Blasa M., Candiracci M., Accorsi A., Piacentini M. P., Piatti E. (2007) Honey flavonoids as protection agents against oxidative damage to human red blood cells. Food Chemistry 104: 1635-1640.

  • Bogdanov S., Jurendic T., Sieber R., Gallman P. (2008) Honey Nutrition and health: a review. Journal of the American College of Nutrition 27: 677-689.

  • Brand-Williams W., Cuvelier M. E., Berset C. (1995) Use of free radical method to evaluate antioxidant activity. Lebensmittel Food Science and Technology 28: 25-30.

  • Buratti S., Benedetti S., Cosio M. S. (2007) Evaluation of the antioxidant power of honey, propolis and royal jelly by amperometric flow injection analysis. Talanta 71: 1387-1392. [Web of Science]

  • CODEX Stan 12 (2001) Codex Stan 12-1981. Adopted in 1981. Revisions 1987 and 2001. 17 pp. Available at: http://teca.fao.org/resource/codex-alimentarius-honeystandard

  • Fagúndez G. A., Caccavari M. A. (2006) Pollen analysis of honeys from the central zone of Argentine province of Entre Ríos. Grana 45: 305-320.

  • Fuenmayor C. A., Garcés L. D., Díaz-Moreno A., Quicazán M. C. (2011) A comparative study of the antioxidant activity of bee pollens from the world. In: Proceedings of 42nd International Apicultural Congress of Apimondia. Buenos Aires - Argentina. 21-25 September 2011: 248.

  • Georgé S., Brat P., Alter P., Amiot M. (2005) Rapid determination of polyphenols and vitamina C in plant-derived products. Journal of Agricultural And Food Chemistry 53: 1370-1373.

  • Gheldof H., Wang X., Engeseth N. J. (2002) Identification and quantification of antioxidant component of honeys from various floral sources. Journal of Agricultural and Food Chemistry 50(21): 5870-5877. [Crossref]

  • González Lorente M., De Lorenzo Carretero C., Pérez Martin R. A. (2008) Sensory attributes and antioxidant capacity of Spanish Honeys. Journal of Sensory Studies 23: 293-302.

  • Halliwell B., Gutteridge J., Aruoma O. (1987) The desoxyrribosa method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry 165: 215-219.

  • Hamdy A. A, Ismail H., AL- Ahwal A., Gomaa N. (2009) Determination of Flavonoids and Phenolic Acid Contents of Clover, Cotton and Citrus Floral Honeys. The Journal of the Egyptian Public Health Association 84(3&4): 245-259.

  • Iurlina M. O., Saiz I. A., Fangio F., Fritz R. (2011) Mieles monoflorales de trébol. Caracterización del potencial antioxidante, contenido de flavonoides y color. In: Proceedings of 42nd International Apicultural Congress of Apimondia. Buenos Aires - Argentina. 21-25 Septembre 2011: 244.

  • Johnson D. E. (2004) Métodos multivariados aplicados al análisis de datos. International Thomson Publishing. Madrid. 566 pp.

  • Kaškonienė V., Maruška A., Kornyšova O., Charczun N., Ligor M., Buszewski B. (2009) Quantitative and qualitative determination of phenolic compounds in honey. Chemical Technology 3(52): 74-80.

  • Liu X., Zhao M., Wang J., Yang B., Jiang Y. (2008) Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. Journal of Food Composition and Analysis 21(3): 219-228. [Crossref] [Web of Science]

  • Liviu M., Dezmirean D., Bobis O. (2011) Authenticity study of honey using specific markers for product traceability. In: Proceedings of 42nd International Apicultural Congress of Apimondia. Buenos Aires - Argentina. 21-25 September 2011: 242.

  • Louveaux J., Maurizio A., Vorwohl G. (1978) Methods of Melissopalynology. Bee World 59: 139-157.

  • McKibben, J., Engeseth, N. J. (2002) Honey as a protective agent against lipid oxidation in ground turkey. Journal of Agricultural and Food Chemistry 50(3): 592-595. [Crossref]

  • Meda A., Lamien C. Romito M., Millongo J. Nacoulma O. (2005) Determination of the total phenolic, flavonoids and proline contents in Burkina Fasan honey, as well as their radical scavening activity. Food Chemistry 91: 571-577.

  • Montenegro G., Santander F., Jara, C., Nuñez G., Fredes C. (2013) Actividad antioxidante y antimicrobiana de mieles monoflorales de plantas nativas chilenas. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 12(3): 257-268.

  • Muñoz O., Copaja S., Speisky H., Peña R., Montenegro G. (2007) Contenido de flavonoides y compuestos fenólicos de mieles chilenas e índice antioxidante. Quimica Nova 30(4): 848-851. [Crossref]

  • Otilia B., Socaciu C., Mărghitas L., Dezmirean D. (2005) Correlations between total phenols, flavonoids, colour intensity and botanical origin of some honeys from Transilvania region. Bulletin of the University of Agricultural Science and Veterinary Medicine 61: 349-353..

  • Oyaizu M. (1986) Studies on the products of browning reaction prepared from glucosamine. Japan Journal of Nutrition 44: 307-315.

  • Pérez R. A., Iglesias M. T., Pueyo E., González M., de Lorenzo C. (2007) Amino acid composition and antioxidant capacity of Spanish honeys. Journal of Agricultural Food Chemistry 55(2): 360-365. [Crossref]

  • Prior R. L., Wu X. L., Schaich K. (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry 53(10): 4290-4302. [Crossref]

  • Rodríguez A., Pérez E., Vit P. (2007) Capacidad antioxidante de mieles venezolanas de los géneros Apis, Melipona y Tetragonisca, evaluados por tres métodos. Revista del Instituto Nacional de Higiene Rafael Rangel 38(2): 30-34.

  • Roginsky V., Lissi E. (2005) Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry 92: 235-254.

  • SAS Institute (2011) SAS/OR® 9.3 User’s Guide: Mathematical Programming. SAS Institute, Cary. USA.

  • Singleton V. L., Orthofer R., Lamuela Raventos R. M. (1999) Analysis of total phenol and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymology 299: 152-178.

  • Sosa Martínez R., Tenori Borroto E., Marrero Chang O., Águila Gímenez E., Camacho Bordón S., Morales Montero A. (2009) Determinación de compuestos fenólicos, metales y su efecto sobre el potencial antioxidante y tóxico en mieles procedentes de Apis mellifera. In: Proceedings of III Congreso Cubano de Apicultura. La Habana, Cuba. 9-13 March 2009: 154.

  • Vasco C., Ruales J., Kamal Eldin A. (2008) Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chemistry 111: 816-823.

  • Vela L., de Lorenzo C., Pérez, R. A. (2007) Antioxidant capacity of Spanish honeys and its correlation with some physicochemical parameters and poliphenolic content. Journal of the Science of Food and Agriculture 87: 1069-1075. [Web of Science]

  • Vit P., Gutiérrez M. G., Titera D., Vendar M., Rodríguez Malaver A. J. (2008) Mieles checas categorizadas según su actividad antioxidante. Acta de Bioquímica Clínica Latinoamericana 42(2): 237-244.

  • Woisky R., Salatino A. (1998) Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research 37: 99-105.

  • Won Lee K., Jun Kim Y., Joo Lee H., Yong Lee C. (2003) Cocoa has more phenolic phytochemicals and higher antioxidant capacity than teas and red wine. Journal of Agricultural and Food Chemistry 41: 792-795.

  • Wu X., Beecher G. R., Holden J. M., Haytowitz D. B., Gebardt E. S., Prior R. L. (2004) Lypophilic and hydrophilic antioxidant capacity of common foods in the United States. Journal of Agricultural and Food Chemistry 52: 4026-4037.

About the article

Received: 2013-07-25

Accepted: 2014-02-24

Published Online: 2014-05-27

Published in Print: 2014-06-01

Citation Information: Journal of Apicultural Science, ISSN (Online) 2299-4831, DOI: https://doi.org/10.2478/jas-2014-0010. Export Citation

© by María C. Ciappini. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY-NC-ND 3.0)

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