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Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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2299-4831
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Determination of Antioxidant Capacity, Flavonoids, and Total Phenolic Content in Eucalyptus and Clover Honeys

María C. Ciappini
  • Corresponding author
  • CIDTA - Universidad Tehnológica Nacional – Facultad Regional Rosario – E. Zeballos 1341 – S2000BUN Rosario – Argentina
  • Email:
/ Fernando S. Stoppani
  • CIDTA - Universidad Tehnológica Nacional – Facultad Regional Rosario – E. Zeballos 1341 – S2000BUN Rosario – Argentina
Published Online: 2014-05-27 | DOI: https://doi.org/10.2478/jas-2014-0010

Abstract

Polyphenolic compounds reportedly produce physiological effects that are beneficial to health. Bee products are particularly rich in polyphenolic compounds. We determined the antioxidant capacity and the phenolic and flavonoid compounds content of 81 samples of honey. We used the Folin-Ciocalteu reagent method to evaluate the total phenolic content. The antioxidant activities were evaluated using in vitro scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH ) and hydroxyl radicals (OH ), Trolox equivalent antioxidant capacity (TEAC ), and ferric-reducing antioxidant capacity (FRAC ). Total phenolic content ranged from 40.3 to 193.0 mg gallic acid equivalents (GAE )/100 g; the flavonoid content varied from 1.4 to 7.5 mg quercetin equivalents (QE)/100 g. Eucalyptus honeys exhibited significantly higher phenolic content and free radical-scavenging activity than clover honey samples (p<0.05 for all). Principal component analysis explained 73% of the differences observed in antiradical activity with respect to floral origin. Total phenolic content may be more useful than the radical-scavenging assay for detecting antioxidant capacity in honey; it also represents the variable that most appropriately discriminated among these honeys.

Keywords: DPPH; ferric-reducing antioxidant capacity; flavonoids in honey; hydroxyl radicals; polyphenols; Trolox equivalent antioxidant capacity

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About the article

Received: 2013-07-25

Accepted: 2014-02-24

Published Online: 2014-05-27

Published in Print: 2014-06-01



Citation Information: Journal of Apicultural Science, ISSN (Online) 2299-4831, DOI: https://doi.org/10.2478/jas-2014-0010. Export Citation

© by María C. Ciappini. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY-NC-ND 3.0)

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