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Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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An Approach for Routine Analytical Detection of Beeswax Adulteration Using FTIR-ATR Spectroscopy

Lidija Svečnjak
  • Corresponding author
  • University of Zagreb Faculty of Agriculture, Department of Fisheries, Apiculture and Special Zoology, 10000 Zagreb, Croatia
  • Email:
/ Goran Baranović
  • Rudjer Boskovic Institute, Division of Organic Chemistry and Biochemistry (Laboratory of Molecular Spectroscopy), 10000 Zagreb, Croatia
/ Marko Vinceković
  • University of Zagreb Faculty of Agriculture, Department of Chemistry, 10000 Zagreb, Croatia
/ Saša Prđun
  • University of Zagreb Faculty of Agriculture, Department of Fisheries, Apiculture and Special Zoology, 10000 Zagreb, Croatia
/ Dragan Bubalo
  • University of Zagreb Faculty of Agriculture, Department of Fisheries, Apiculture and Special Zoology, 10000 Zagreb, Croatia
/ Ivana Tlak Gajger
  • University of Zagreb, Faculty of Veterinary Medicine, Department of Biology and Pathology of Fish and Bees, 10000 Zagreb, Croatia
Published Online: 2015-12-09 | DOI: https://doi.org/10.1515/jas-2015-0018


Although beeswax adulteration represents one of the main beeswax quality issues, there are still no internationally standardised analytical methods for routine quality control. The objective of this study was to establish an analytical procedure suitable for routine detection of beeswax adulteration using FTIR-ATR spectroscopy. For the purpose of this study, reference IR spectra of virgin beeswax, paraffin, and their mixtures containing different proportions of paraffin (5 - 95%), were obtained. Mixtures were used for the establishment of calibration curves. To determine the prediction strength of IR spectral data for the share of paraffin in mixtures, the Partial Least Squares Regression method was used. The same procedure was conducted on beeswax-beef tallow mixtures. The model was validated using comb foundation samples of an unknown chemical background which had been collected from the international market (n = 56). Selected physico-chemical parameters were determined for comparison purposes. Results revealed a strong predictive power (R2 = 0.999) of IR spectra for the paraffin and beef tallow share in beeswax. The results also revealed that the majority of the analysed samples (89%) were adulterated with paraffin; only 6 out of 56 (11%) samples were identified as virgin beeswax, 28% of the samples exhibited a higher level of paraffin adulteration (>46% of paraffin), while the majority of the analysed samples (50%) were found to be adulterated with 5 - 20% of paraffin. These results indicate an urgent need for routine beeswax authenticity control. In this study, we demonstrated that the analytical approach defining the standard curves for particular adulteration levels in beeswax, based on chemometric modelling of specific IR spectral region indicative for adulteration, enables reliable determination of the adulterant proportions in beeswax.

Keyword: adulterants share detection; beeswax adulteration; fingerprint region; FTIR-ATR spectroscopy


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About the article

Received: 2014-10-08

Accepted: 2015-07-10

Published Online: 2015-12-09

Published in Print: 2015-12-01

Citation Information: Journal of Apicultural Science, ISSN (Online) 2299-4831, DOI: https://doi.org/10.1515/jas-2015-0018.

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© by Lidija Svečnjak. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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