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Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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Toxicity of Spirotetramat on Solitary Bee Larvae, Osmia Cornuta (Hymenoptera: Megachilidae), in Laboratory Conditions

Fabio Sgolastra
  • Corresponding author
  • Department of Agricultural Science, Alma Mater Studiorum University of Bologna, viale Fanin 42, 40127 Bologna, Italy
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/ Simone Tosi
  • Department of Agricultural Science, Alma Mater Studiorum University of Bologna, viale Fanin 42, 40127 Bologna, Italy
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/ Piotr Medrzycki
  • Agricultural Research Council, Honey Bee and Silkworm Research Unit, via di Saliceto 80, 40128 Bologna, Italy
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/ Claudio Porrini
  • Department of Agricultural Science, Alma Mater Studiorum University of Bologna, viale Fanin 42, 40127 Bologna, Italy
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/ Giovanni Burgio
  • Department of Agricultural Science, Alma Mater Studiorum University of Bologna, viale Fanin 42, 40127 Bologna, Italy
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Published Online: 2015-12-09 | DOI: https://doi.org/10.1515/jas-2015-0024


In this paper we assessed, under laboratory conditions, the toxicity of an active substance on solitary bee larvae of Osmia cornuta (Hymenoptera: Megachilidae). A field-realistic dose of the systemic insecticide spirotetramat was applied to the mass provisions. The insecticide’s effects on several life-cycle parameters were studied in males and females. Our results showed a significantly shorter post-emergence longevity in bees exposed to spirotetramat during the larval stage, compared to the control. The observed reduction in longevity was 18 and 15%, respectively, in males and females. Mortality rate and other biological traits (larval and spinning duration, emergence time, food/body conversion rate) did not show significant differences between the two treatments. The method described in this study can be used to test the effects of toxic substances (i.e. agrochemicals) on bees. Moreover, it can constitute a basis for the development of a standardised protocol in the first tier of the Environmental Risk Assessment for solitary bees.

Keywords: bee; ecotoxicology; insecticide; pesticide; toxicity test


  • Abbott V. A., Nadeau J. L., Higo H. A., Winston M. L. (2008) Lethal and sublethal effects of imidacloprid on Osmia lignaria and clothianidin on Megachile rotundata (Hymenoptera: megachilidae). Journal of Economic Entomology 101: 784-796.Web of ScienceCrossrefGoogle Scholar

  • Arena M., Sgolastra F. (2014) A meta-analysis comparing the sensitivity of bees to pesticides. Ecotoxicology 23: 324-334.CrossrefWeb of ScienceGoogle Scholar

  • Babendreier D., Kalberer N., Romeis J., Fluri P., Bigler F. (2004) Pollen consumption in honey bee larvae: a step forward in the risk assessment of transgenic plants. Apidologie 35: 293-300.CrossrefGoogle Scholar

  • Bosch J., Kemp W. P. (2000) Development and emergence of the orchard pollinator, Osmia lignaria (Hymenoptera, Megachilidae). Environmental Entomology 29: 8-13.CrossrefGoogle Scholar

  • Bosch J., Kemp W. P. (2001) How to manage the blue orchard bee as an orchard pollinator. Sustainable Agricultural Network. Beltsville, Maryland. 98 pp.Google Scholar

  • Bosch J., Kemp W. P. (2002) Developing and establishing bee species as crop pollinators: the example of Osmia spp. (Hymenoptera: Megachilidae) and fruit trees. Bulletin of Entomological Research 92: 3-16.Google Scholar

  • Bosch J., Kemp W. P. (2004) Effect of pre-wintering and wintering temperature regimes on weight loss, survival, and emergence time in the mason bee Osmia cornuta (Hymenptera: Megachilidae). Apidologie 35: 469-479.CrossrefGoogle Scholar

  • Bosch J., Vicens N. (2002) Body size as an estimator of production costs in a solitary bee. Ecological Entomology 27: 129-137.CrossrefGoogle Scholar

  • Bosch J., Vicens N. (2005) Sex allocation in the solitary bee Osmia cornuta: do females behave in agreement with Fisher’s theory? Behavioral Ecology and Sociobiology 59: 124-132.CrossrefGoogle Scholar

  • Bosch J., Vicens N. (2006) Relationship between body size, provisioning rate, longevity and reproductive success in females of the solitary bee Osmia cornuta. Behavioral Ecology and Sociobiology 60: 26-33.CrossrefGoogle Scholar

  • Bosch J., Sgolastra F., Kemp W. P. (2008) Life Cycle Ecophysiology of Osmia Mason Bees Used as Crop Pollinators. In: James R. R., Pitts-Singer T. L. (Eds.) Bee Pollination in Agricultural Ecosystems. Oxford University Press. Oxford, UK: 83-104.Google Scholar

  • Bosch J., Sgolastra F., Kemp W. P. (2010) Timing of eclosion affects diapause development, fat body consumption and longevity in Osmia lignaria, a univoltine, adultwintering solitary bee. Journal of Insect Physiology 56: 1949-1957.Web of ScienceCrossrefGoogle Scholar

  • Brittain C., Potts S. G. (2011) The potential impacts of insecticides on the life-history traits of bees and the consequences for pollination. Basic and Applied Ecology 12: 321-331.CrossrefWeb of ScienceGoogle Scholar

  • Crailsheim K., Brodschneider R., Aupinel P., Behrens D., Genersch E., Vollmann J., Riessberger-Gallé U. (2012) Standard methods for artificial rearing of Apis mellifera larvae. Journal of Apicultural Research 52: 1-15.Google Scholar

  • Dmochowska K., Giejdasz K., Fliszkiewicz M., Żółtowska K. (2012) Changes in antioxidative system of red mason bee (Osmia rufa) (Hymenoptera: Megachilidae) induced by artificially elongated postdiapause. Journal of Apicultural Science 56: 99-106.CrossrefWeb of ScienceGoogle Scholar

  • EFSA (2013) EFSA Guidance Document on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees). EFSA Journal 11(7): 3295. 266 pp.Google Scholar

  • European Commissiom (2009) Regulation EC No 1107/2009 of the European Parliament and of the Council. Official Journal of European Union 309: 1-50.Google Scholar

  • Gallai N., Salles J. M., Settele J., Vaissiere B. E. (2009) Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecological Economics 68: 810-821.Web of ScienceCrossrefGoogle Scholar

  • Garibaldi L. A., Steffan-Dewenter I., Winfree R., Aizen M. A., Bommarco R., Cunningham S. A., Kremen C., Carvalheiro L. G., Harder L. D., Afik O., Bartomeus I., Benjamin F., Boreux V., Cariveau D., Chacoff N. P., Dudenhoffer J. H., Freitas B. M., Ghazoul J., Greenleaf S., Hipolito J., Holzschuh A., Howlett B., Isaacs R., Javorek S. K., Kennedy C. M., Krewenka K. M., Krishnan S., Mandelik Y., Mayfield M. M., Motzke I., Munyuli T., Nault B. A., Otieno M., Petersen J., Pisanty G., Potts S. G., Rader R., Ricketts T. H., Rundlof M., Seymour C. L., Schuepp C., Szentgyorgyi H., Taki H., Tscharntke T., Vergara C. H., Viana B. F., Wanger T. C., Westphal C., Williams N., Klein A. M. (2013) Wild pollinators enhance fruit set of crops regardless of honey bee abundance. Science 339: 1608-1611.Web of ScienceGoogle Scholar

  • Gradish A. E., Scott-Dupree C. D., Cutler G. C. (2012). Susceptibility of Megachile rotundata to insecticides used in wild blueberry production in Atlantic Canada. Journal of Pest Science 85: 133-140.CrossrefWeb of ScienceGoogle Scholar

  • Hodgson E. W., Pitts-Singer T. L., Barbour J. D. (2011) Effects of the insect growth regulator, novaluron on immature alfalfa leafcutting bees, Megachile rotundata. Journal of Insect Science 11: 43. Available at: http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1050&context=ent_pubs CrossrefGoogle Scholar

  • Huntzinger C. I., James R. R., Bosch J., Kemp W. P. (2008) Laboratory biossays to evaluate fungicides for chalkbrood control in larvae of the alfalfa leafcutting bee (Hymenoptera; Megachilidae). Journal of Economic Entomology 101: 660-667.CrossrefWeb of ScienceGoogle Scholar

  • Klein A. M., Vaissiere B. E., Cane J. H., Steffan-Dewenter I., Cunningham S. A., Kremen C., Tscharntke T. (2007) Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society B 274: 303-313.Web of ScienceGoogle Scholar

  • Konrad R., Ferry N., Gatehouse A. M. R., Babendreier D. (2008) Potential effects of oilseed rape expressing oryzacystatin-1 (OC-1) and of purified insecticidal proteins on larvae of the solitary bee Osmia bicornis. PLoS ONE 3(7): e2664.Web of ScienceGoogle Scholar

  • Ladurner E., Bosch J., Kemp W. P., Maini S. (2005) Assessing delayed and acute toxicity of five formulated fungicides to Osmia lignaria Say and Apis mellifera. Apidologie 36: 449-460.CrossrefGoogle Scholar

  • Ladurner E., Bosch J., Kemp W. P., Maini S. (2008) Foraging and Nesting Behavior of Osmia lignaria (Hymenoptera: Megachilidae) in the Presence of Fungicides: Cage Studies. Journal of Economic Entomology 101: 647-653.Web of ScienceCrossrefGoogle Scholar

  • Maus C. (2008) Ecotoxicological profile of the insecticide spirotetramat. Bayer Crop Science Journal 61: 159-180.Google Scholar

  • Peach M. L., Alston D. G., Tepedino V. J. (1995) Sublethal effects of carbaryl bran bait on nesting performance, parental investment, and offspring size and sex ratio of the alfalfa leafcutting bee (Hymenoptera: Megachilidae). Environmental Entomology 24: 34-39.CrossrefGoogle Scholar

  • Peters D. S. (1977) Systematik und Zoogeographie der westpaleärktischen Arten von Osmia’s str., Monosmia, und Orientosmia. Senckenbergiana Biologica 58: 287-346.Google Scholar

  • Radmacher S., Strohm E. (2010) Factors affecting offspring body size in the solitary bee Osmia bicornis (Hymenoptera, Megachilidae). Apidologie 41: 169-177.Web of ScienceCrossrefGoogle Scholar

  • Radmacher S., Strohm E. (2011) Effects of constant and fluctuating temperatures on the development of the solitary bee Osmia bicornis (Hymenoptera: Megachilidae). Apidologie 42: 711-720.Web of ScienceCrossrefGoogle Scholar

  • Rogers D., Williams G., Lam C., Fischer D., Hackenberg D. (2010) Movento®, Citrus and Honey Bees. Results of a successful cooperative study. In: Proceedings of the American Bee Research Conference. Orlando-USA. 14-15 January 2010. American Bee Journal: 506.Google Scholar

  • Rundlöf M., Andersson G. K. S., Bommarco R., Fries I., Hederström V., Herbertsson L., Jonsson O., Klatt B. K., Pedersen T. R., Yourstone J., Smith H. G. (2015) Seed coating with a neonicotinoid insecticide negatively affects wild bees. Nature 521(7550): 77-80.Web of ScienceGoogle Scholar

  • Sandrock C., Tanadini L. G., Pettis J. S., Biesmeijer J. C., Potts S. G., Neumann P. (2014) Sublethal neonicotinoid insecticide exposure reduces solitary bee reproductive success. Agricultural and Forest Entomology 16: 119-128. Web of ScienceCrossrefGoogle Scholar

  • Schindler M., Diestelhorst O., Härtel S., Saure C., Schanowski A., Schwenninger H. R. (2013) Monitoring agricultural ecosystems by using wild bees as environmental indicators. Biorisk 8: 39-51.CrossrefGoogle Scholar

  • Sgolastra F., Kemp W. P., Maini S., Bosch J. (2012) Duration of prepupal summer dormancy regulates synchronization of adult diapause with winter temperatures in bees of the genus Osmia. Journal of Insect Physiology 58: 924-933.Web of ScienceCrossrefGoogle Scholar

  • Sgolastra F., Kemp W. P., Buckner J. S., Pitts-Singer T. L., Maini S., Bosch J. (2011) The long summer: Pre-wintering temperatures affect metabolic expenditure and winter survival in a solitary bee. Journal of Insect Physiology 57: 1651-1659. Statistica ver. 7. (2004) StatSoft Inc.Web of ScienceCrossrefGoogle Scholar

  • Tasei J. N., Carre S., Moscatelli B., Grondeau C. (1988) Recherche de la D.L. 50 de la deltamethrine (Decis) chez Megachile rotundata F. Abeille pollinistatrice de la luzerne (Medicago sativa L.) et des effets de doses infralethales sur les adultes et les larves. Apidologie 19 (3): 291-306.CrossrefGoogle Scholar

  • Tesoriero D., Maccagnani B., Santi F., Celli G. (2003) Toxicity of three pesticides on larval instars of Osmia cornuta: preliminary results. Bulletin of Insectology 56: 169-171.Google Scholar

  • Vicens N., Bosch J. (2000) Nest site orientation and relocation of populations of the orchard pollinator Osmia cornuta (Hymenoptera: Megachilidae). Environmental Entomology 29: 69-75 .CrossrefGoogle Scholar

  • Wasielewski O., Wojciechowicz T., Giejdasz K., Krishnan N. (2013) Overwintering strategies in the red mason solitary bee-physiological correlates of midgut metabolic activity and turnover of nutrient reserves in females of Osmia bicornis. Apidologie 44: 642-656. CrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2015-03-06

Accepted: 2015-10-07

Published Online: 2015-12-09

Published in Print: 2015-12-01

Citation Information: Journal of Apicultural Science, Volume 59, Issue 2, Pages 73–83, ISSN (Online) 2299-4831, DOI: https://doi.org/10.1515/jas-2015-0024.

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© by Fabio Sgolastra. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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