Jump to ContentJump to Main Navigation
Show Summary Details

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

2 Issues per year


IMPACT FACTOR 2015: 0.571
5-year IMPACT FACTOR: 0.779

SCImago Journal Rank (SJR) 2015: 0.392
Source Normalized Impact per Paper (SNIP) 2015: 0.470
Impact per Publication (IPP) 2015: 0.694

Open Access
Online
ISSN
2299-4831
See all formats and pricing

The Ability of Honey Bee Drones to Ejaculate

Krystyna Czekońska
  • Corresponding author
  • Department of Pomology and Apiculture, Agricultural University, 29 Listopada 54, 31-425 Kraków, Poland
  • Email:
/ Bożena Chuda-Mickiewicz
  • Department of Zoology and Apiculture, West Pomeranian University of Technology, Doktora Judyma 20, 71-466 Szczecin, Poland
Published Online: 2015-12-09 | DOI: https://doi.org/10.1515/jas-2015-0027

Abstract

The effectiveness of two methods of collecting semen from honeybee Apis mellifera drones was compared, and the reasons for problems with ejaculating semen were analysed. Among 275 drones, 100 were stimulated to release semen using a manual method, 100 with the use of chloroform, and from 75 drones the reproductive organs were dissected for analysis and evaluation. It was found that the principal causes of problems that drones had with ejaculating their semen were anatomical changes or a delay in the development of the mucus glands. It was also found that the method employing chloroform was less efficient in the first phase of eversion of the endophallus, compared with the manual method. The method with the use of chloroform allows the determination of the proportion of drones, which do not evert the endophallus because of poor or delayed development of mucus glands, as well as the proportion of drones which evert the organ, but do not ejaculate semen because of the absence of semen in the seminal vesicles.

Keywords: Apis mellifera; drone; eversion; reproductive organ

References

  • Abdelkader F. B., Kairo G., Tchamitchian S., Cousin M., Senechal J., Crauser D., Le Conte Y., Belzunces L. P., Barbouche N., Brunet J. L. (2014) Semen quality of honey bee drones maintained from emergence to sexual maturity under laboratory, semi-field and field conditions. Apidologie 45(2): 215-223. DOI: 10.1007/s13592-013-0240-7 [Web of Science] [Crossref]

  • Bieńkowska M., Panasiuk B., Węgrzynowicz P., Gerula D. (2011) The effect of different thermal conditions on drone semen quality and number of spermatozoa entering the spermatheca of queen bee. Journal of Apiculture Science 55(2): 161-168.

  • Bishop G. H. (1920) Fertilization in the honeybee. I. The male sexual organs: their histological structure and physiological functioning. Journal of Experimental Zoology 31: 225-265. [Crossref]

  • Boes K. E. (2010) Honeybee colony drone production and maintenance in accordance with environmental factors: an interplay of queen and worker decisions. Insects Sociaux 57: 1-9. DOI: 10.1007/s00040-009-0046-9 [Web of Science] [Crossref]

  • Carreck N. L., Andree M., Brent C. S., Cox-Foster D., Dade H. A., Ellis J. D., Hatijina F., vanEnglesdorp D. (2013) Standard methods for Apis mellifera anatomy and dissection. Journal of Apiculture Research 52(4): 1-40. DOI:10.3896/ IBRA.1.52.4.03 [Web of Science] [Crossref]

  • Chuda-Mickiewicz B., Prabucki J. (1993) Próba określenia wpływu pory wychowu trutni na wartość inseminowanych matek. Pszczelnicze Zeszyty Naukowe 37: 41-49.

  • Cobey S. W., Tarpy D. R., Woyke J. (2013) Standard methods for instrumental insemination of Apis mellifera queens. Journal of Apiculture Research 52(4): 1-18. DOI: 10.3896/IBRA.1.52.4.09 [Web of Science] [Crossref]

  • Colonello N. A., Hartfelder K. (2005) She’s my girl - male accessory gland products and their function in the reproductive biology of social bees. Apidologie 36: 231-244. [Crossref]

  • Couvillon M. J., Hughes W. O., Perez-Sato J. A., Martin S. J., Roy G. G., Ratnieks F. L. (2010) Sexual selection in honey bees: colony variation and the importance of size in male mating success. Behavioral Ecology 21(3): 520-525. DOI:10.1093/beheco/arq016 [Web of Science] [Crossref]

  • Cruz-Landim C. D., Dallacqua R. P. (2005) Morphology and protein patterns of honey bee drone accessory glands. Genetics and Molecular Research 4(3): 473-481. DOI: 10.1002/jemt.22024 [Crossref]

  • Cruz-Landim D., Roat T. C., Fernadez F. C. (2012) Virus present in the reproductive tract of asymptomatic drones of honey bee (Apis mellifera L.), and possible infection of queen during mating. Microscopy research and technique 75(7): 986-990. [Web of Science]

  • Czekońska K., Chuda-Mickiewicz B., Chorbiński P. (2013) The effect of brood incubation temperature on the reproductive value of honey bee (Apis mellifera). Journal of Apiculture Research 52: 96-105. DOI: 10.3896/ IBRA.1.52.4.09 [Crossref]

  • Czekońska K., Chuda-Mickiewicz B., Samborski J. (2015) Quality of honey bee drones reared in colonies with limited and unlimited access to pollen. Apidologie 46(1): 1-9. DOI: 10.1007/s13592-014-0296-z [Crossref] [Web of Science]

  • Dade H. A. (1994) Dissection of the drone. In: Dade H. A. (Ed.) Anatomy and dissection of the honeybee. International Bee Research Association: 121-122.

  • De Jong D. (1997) Mites: Varroa and other parasites of brood. In: Morse R. A., Flottum K. (Eds.) Honey Bee Pests, Predators, and Diseases. A.I. Root Company. New York: 200-218.

  • Drescher W. (1976) Rearing and maintaining queens and drones. In: Ruttner F. (Ed.) The instrumental insemination of the queen bee. APIMONDIA, International Beekeeping Technology and Economy Institute. Bucharest: 25-38.

  • Duay P. R., De Jong D., Engels W. (2002) Decreased flight performance and sperm production in drones of the honey bee (Apis mellifera) slightly infested by Varroa destructor mites during pupal development. Genetics and Molecular Research 1(3): 227-232.

  • Duay P. R., De Jong D., Engels W. (2003) Weight loss in drone pupae (Apis mellifera) multiply infested by Varroa destructor mites. Apidologie 34: 61-65. [Crossref]

  • Fievet J., Tentcheva D., Gauthier L., De Miranda J., Cousserans F., Colin M. E., Bergoin M. (2006) Localization of deformed wing virus infection in queen and drone Apis mellifera L. Virology Journal 3(16): 1-5. DOI:10.1186/1743-422X-3-16 [Crossref]

  • Free J. B., Williams I. H. (1975) Factors determining the rearing and rejection of drones by the honeybee colony. Animal Behaviour 23(3): 650-675. [Crossref]

  • Fukuda H., Ohtani T. (1977) Survival and life span of drone honeybees. Researches on Population Ecology 19: 51-68. [Crossref]

  • Gençer H. V. Firatli Ç. (2005) Reproductive and morphological comparisons of drones reared in queenright and laying worker colonies. Journal of Apiculture Research 44(4): 163-167. DOI: 10.3896/IBRA.1.44.4.06 [Crossref]

  • Harbo J. R. (1986) Propagation and instrumental insemination. In: Rinderer T. E. (Ed.) Bee genetics and breeding. Academic Press. Orlando: 361-389.

  • Jaycox E. R. (1961) The effects of various foods and temperatures on sexual maturity of the drone honey bee (Apis mellifera). Annals of the Entomological Society of America 54(4): 519-52.

  • Koeniger G., Koeniger N., Phiancharoen M. (2011) Comparative reproductive biology of honeybees. In: Hepburn H. R., Radloff S. E. (Eds.) Honeybees of Asia. Springer-Verlag. Berlin Heidelberg: 159-206.

  • Koeniger G., Wissel M., Herth W. (1990) Cornual secretion on the endophallus of the honeybee drone (Apis mellifera L). Apidologie 21(3): 185-191. [Crossref]

  • Kraus F. B., Neumann P., Scharpenberg H., Van Praagh J., Moritz R. F. A. (2003) Male fitness of honeybee colonies (Apis mellifera L.). Evolutionary Biology 16(5): 914-920. [Crossref]

  • Kurennoi N. M. (1953) When are drones sexually mature? Pchelovodstvo 30(11): 28-32 [in Russian].

  • Laidlaw H. H. Jr. (1977) Instrumental insemination of honey bee queens. Dadant and Sons. Hamilton, IL, USA. 144 pp.

  • Mackensen O., Ruttner F. (1976) The insemination procedure. In: Ruttner F (Ed.). The instrumental insemination of the queen bee. APIMONDIA, International Beekeeping Technology and Economy Institute. Bucharest: 69-86.

  • Mazeed A. M. (2011) Morphometry and number of spermatozoa in drone honeybees (Hymenoptera: Apidae) reared under different conditions. European Journal of Entomology 108(4): 673-676. DOI:10.14411/ eje.2011.085 [Crossref]

  • Mazeed A. M., Mohanny K. M. (2010) Some reproductive characteristics of honeybee drones in relation to their ages. Entomological Research 40: 245-250. DOI: 10.1111/j.1748-5967.2010.00297.x [Crossref]

  • Mindt B. (1962) Untersuchung über das Leben der Drohnen, insbesondere Ernährung und Geschlechtsreife. Zeitscrift für Bienenforsch 6 (1): 9-33.

  • Moors L., Spaas O., Koeniger G., Billen J., (2005) Morfological and ultrastructural changes in the mucus glands of Apis mellifera drones during pupal development and sexual maturation. Apidologie 36: 245-254. [Crossref]

  • Retschnig G., Williams G. R., Mehmann M. M., Yañez O., De Miranda J. R., Neumann P. (2014) Sex-specific differences in pathogen susceptibility in honey bees (Apis mellifera). PloS One 9(1): e85261. DOI:10.1371/ journal.pone.0085261 [Web of Science]

  • Rhodes J. W., Harden S., Spooner-Hart R., Anderson D. L., Wheen G. (2011) Effects of age, season and genetics on semen and sperm production in Apis mellifera drones. Apidologie 42(1): 29-38. DOI: 10.1051/apido/2010026 [Web of Science] [Crossref]

  • Rueppell O., Fondrk M. K., Page R. E. (2005) Biodemographic analysis of male honey bee mortality. Aging cell 4(1): 13-19. [Crossref]

  • Rueppell O., Page R. E., Fondrk M. K. (2006) Male behavioural maturation rate responds to selection on pollen hoarding in honeybees. Animal Behaviour 71(1): 227-234. DOI:10.1016/j.anbehav.2005.05.008 [Crossref]

  • Ruttner F. Tryasko V. V. (1976) Anatomy and Physiology of reproduction. In: Ruttner F. (Ed.). The Instrumental Insemination of Queen Bee. APIMONDIA, International Beekeeping Technology and Economy Institute. Bucharest: 11-24.

  • Snodgrass R. E. (1956) Anatomy of the honey bee. Cornell University Press. Ithaca, New York. 352 pp.

  • Sokal R. R., Rohlf F. J. (2009) Biometry. W. H. Freeman and Company. New York. 363 pp.

  • StatSoft, Inc. (2010). Statistica, version 9.1. Available at: www.statsoft.com

  • Stürup M., Baer-Imhoof B., Nash D. R., Boomsma J. J., Baer B. (2013) When every sperm counts: factors affecting male fertility in the honeybee Apis mellifera. Behavioral Ecology 24(5): 1192-1198. DOI:10.1093/beheco/ art049 [Crossref] [Web of Science]

  • Tozetto S. D. O., Rachinsky A., Engels W. (1997) Juvenile hormone promotes flight activity in drones (Apis mellifera carnica). Apidologie 28(2): 77-84. [Crossref]

  • Witherell P. C. (1965) Survival of drones following eversion. Annales de l’Abeille 8(4): 317-320.

  • Woyke J. (1958) The histological structure of the reproductive organs of the drone. The Poznań Society of Friends of Science, Publication of the Section of Agriculture and Silviculture 19(2): 38-89.

  • Woyke J. (2008) Why the eversion of the endophallus of honey bee drone stops at the partly everted stage and significance of this. Apidologie 39(6): 627-636. DOI: 10.1051/apido:2008046 [Web of Science] [Crossref]

  • Woyke J. (2010) Three substances ejected by Apis mellifera drones from everted endophallus and during natural matings with queen bees. Apidologie 41(6): 613-621. DOI: 10.1051/apido/20010007 [Web of Science] [Crossref]

  • Woyke J., Jasinski Z. (1978) Influence of age of drones on the results of instrumental insemination of honeybee queens. Apidologie 9(3): 203-212. [Crossref]

About the article

Received: 2015-07-24

Accepted: 2015-10-07

Published Online: 2015-12-09

Published in Print: 2015-12-01


Citation Information: Journal of Apicultural Science, ISSN (Online) 2299-4831, DOI: https://doi.org/10.1515/jas-2015-0027. Export Citation

© by Krystyna Czekońska. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. (CC BY-NC-ND 4.0)

Comments (0)

Please log in or register to comment.
Log in