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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board Member: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Haim, Abraham / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu

6 Issues per year


CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

Online
ISSN
2191-0286
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Volume 23, Issue 1 (Mar 2012)

Issues

A modified simple method for determination of serum α-tocopherol (vitamin E)

Jameel G. Jargar
  • Department of Physiology, Environmental Health Research Unit, Al-Ameen Medical College, Bijapur, Karnataka, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Shaheenkousar H. Hattiwale
  • Department of Physiology, Environmental Health Research Unit, Al-Ameen Medical College, Bijapur, Karnataka, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Swastika Das
  • Department of Engineering Chemistry, B.L.D.E.A’s V.P. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur, Karnataka, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Salim A. Dhundasi
  • Department of Physiology, Environmental Health Research Unit, Al-Ameen Medical College, Bijapur, Karnataka, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kusal K. Das
Published Online: 2012-01-19 | DOI: https://doi.org/10.1515/jbcpp-2011-0033

Abstract

Background: Vitamin E is one of the important antioxidants linked to regulate various diseases, such as atherosclerosis, hypertension, and male infertility. A relatively simple and economic biochemical modified method has been developed to determine serum α-tocopherol concentration.

Methods: The current modified method is based on previous Baker and Frank method and the method of Martinek by using 2,2′-bipyridyl, ferric chloride, and xylene. The complex of ferrous ions generated in this reaction with 2,2′-bipyridyl is determined by using a plain enzyme-linked immunosorbent assay microplate (non-antibody coated) at 492 nm.

Results: The standard curve of this new modified method shows a linearity with correlation r=0.997 (concentration vs. absorbance). The absorbance of this color complex is directly proportional to the α-tocopherol concentration. The sensitivity of this new modified method has been compared and correlated with Baker and Frank method by using 15 human samples (r=0.99, p<0.0001).

Conclusions: This simple and economic method may be routinely used to analyze α-tocopherol concentration in serum.

Keywords: data analysis; modified method; serum α-tocopherol assay

About the article

Corresponding author: Prof. Kusal K. Das, Department of Physiology, Environmental Health Research Unit, Al-Ameen Medical College, Bijapur-586108, Karnataka, India Phone: (W) +91-8352-272502, +91-8352-271257, Fax: +91-8352-270184


Received: 2011-10-14

Accepted: 2011-12-10

Published Online: 2012-01-19

Published in Print: 2012-03-01


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2011-0033.

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