Journal of Basic and Clinical Physiology and Pharmacology
Editor-in-Chief: Horowitz, Michal
Editorial Board Member: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Haim, Abraham / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu
6 Issues per year
CiteScore 2016: 1.01
SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495
A modified simple method for determination of serum α-tocopherol (vitamin E)
Background: Vitamin E is one of the important antioxidants linked to regulate various diseases, such as atherosclerosis, hypertension, and male infertility. A relatively simple and economic biochemical modified method has been developed to determine serum α-tocopherol concentration.
Methods: The current modified method is based on previous Baker and Frank method and the method of Martinek by using 2,2′-bipyridyl, ferric chloride, and xylene. The complex of ferrous ions generated in this reaction with 2,2′-bipyridyl is determined by using a plain enzyme-linked immunosorbent assay microplate (non-antibody coated) at 492 nm.
Results: The standard curve of this new modified method shows a linearity with correlation r=0.997 (concentration vs. absorbance). The absorbance of this color complex is directly proportional to the α-tocopherol concentration. The sensitivity of this new modified method has been compared and correlated with Baker and Frank method by using 15 human samples (r=0.99, p<0.0001).
Conclusions: This simple and economic method may be routinely used to analyze α-tocopherol concentration in serum.