Jump to ContentJump to Main Navigation
Show Summary Details

Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board Member: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Haim, Abraham / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu

6 Issues per year


SCImago Journal Rank (SJR) 2015: 0.302
Source Normalized Impact per Paper (SNIP) 2015: 0.474
Impact per Publication (IPP) 2015: 0.759

Online
ISSN
2191-0286
See all formats and pricing
Volume 24, Issue 4 (Nov 2013)

Issues

Toxicological evaluations of methanolic extract of Moringa oleifera leaves in liver and kidney of male Wistar rats

Ademola A. Oyagbemi
  • Corresponding author
  • Faculty of Veterinary Medicine, Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Oyo State, Nigeria
  • Email:
/ Temitayo O. Omobowale
  • Faculty of Veterinary Medicine, Department of Veterinary Medicine, University of Ibadan, Oyo State, Nigeria
/ Ibraheem O. Azeez
  • Faculty of Veterinary Medicine, Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Oyo State, Nigeria
/ John O. Abiola
  • Faculty of Veterinary Medicine, Department of Veterinary Medicine, University of Ibadan, Oyo State, Nigeria
/ Rahamon A.M. Adedokun
  • Faculty of Veterinary Medicine, Department of Veterinary Medicine, University of Ibadan, Oyo State, Nigeria
/ Helen O. Nottidge
  • Faculty of Veterinary Medicine, Department of Veterinary Medicine, University of Ibadan, Oyo State, Nigeria
Published Online: 2013-03-18 | DOI: https://doi.org/10.1515/jbcpp-2012-0061

Abstract

Background: This study was conducted to investigate toxicological effects associated with prolonged consumption of Moringa oleifera leaves as a beverage.

Methods: Thirty rats were used in this study. They were grouped into five groups of six rats. Rats in group I received 2 mL/kg body weight (b.w.) of corn oil (vehicle). Animals in groups II, III, IV and V received 50, 100, 200 and 400 mg/kg b.w. of methanolic extract of M. oleifera (MEMO) for 8 weeks. Serum collected was analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein, albumin, globulin, blood urea nitrogen (BUN) and creatinine.

Results: There was a significant (p<0.05) increase in serum total protein and globulin in a dose-dependent manner. Rats that received MEMO at 200 and 400 mg/kg b.w. showed a significant (p<0.05) increase in serum ALT, AST, BUN and creatinine which pointed to hepatic and kidney damage. All experimental animals that received MEMO had a significant (p<0.05) increase in body weight in a dose-dependent manner.

Conclusions: This study therefore confirms for the first time that chronic administration of M. oleifera leaves might predispose to hepatic and kidney damage.

Keywords: kidney; liver; Moringa oleifera; toxicity assessment

References

  • 1.

    D’Souza J, Kulkarni AR. Comparative studies on nutritive values of tender foliage of seedlings and mature plants of Moringa oleifera Lam. J Economic Taxon Botany 1993;17:479–85.

  • 2.

    Viera GH, Mourão JA, Angelo AM, Costa RA, Vieira RH. Antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against Gram positive and Gram negative bacteria. Rev Inst Med Trop Sao Paulo 2010;52:129–32. [PubMed] [Web of Science] [Crossref]

  • 3.

    Promkum C, Kupradinun P, Tuntipopipat S, Butryee C. Nutritive evaluation and effect of Moringa oleifera pod on clastogenic potential in the mouse. Asian Pac J Cancer Prev 2010;11:627–32.

  • 4.

    Oluduro OA, Aderiye BI, Connolly JD, Akintayo ET, Famurewa O. Characterization and antimicrobial activity of 4-(β-D-glucopyranosyl-1→4-α-L-rhamnopyranosyloxy)-benzyl thiocarboxamide; a novel bioactive compound from Moringa oleifera seed extract. Folia Microbiol (Praha) 2010;55:422–6. [Web of Science]

  • 5.

    Debnath S, Biswas D, Ray K, Guha D. Moringa oleifera induced potentiation of serotonin release by 5-HT(3) receptors in experimental ulcer model. Phytomedicine 2011;18:91–5. [Crossref] [Web of Science] [PubMed]

  • 6.

    Ogunsina BS, Radha C, Indrani D. Quality characteristics of bread and cookies enriched with debittered Moringa oleifera seed flour. Int J Food Sci Nutr 2011;62:185–94. [Crossref] [PubMed] [Web of Science]

  • 7.

    Ghasi S, Nwobodo E, Ofili JO. Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed Wistar rats. J Ethnopharmacol 2000;69:21–5. [Crossref]

  • 8.

    Mehta LK, Balaraman R, Amin AH, Bafna PA, Gulati OD. Effect of fruits of Moringa oleifera on the lipid profile of normal and hypercholesterolaemic rabbits. J Ethnopharmacol 2003;86:191–5. [PubMed] [Crossref]

  • 9.

    Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: a food plant with multiple medicinal uses. Phytother Res 2007;21:17–25. [PubMed] [Crossref] [Web of Science]

  • 10.

    Cheenpracha S, Park EJ, Yoshida WY, Barit C, Wall M, Pezzuto JM, et al. Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits. Bioorg Med Chem 2010;18:6598–602. [Crossref] [PubMed] [Web of Science]

  • 11.

    Chumark P, Khunawat P, Sanvarinda Y, Phornchirasilp S, Morales NP, Phivthong-Ngam L, et al. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J Ethnopharmacol 2008;116:439–46. [Crossref] [PubMed] [Web of Science]

  • 12.

    Nambiar VS, Seshadri S. Bioavailability trials of β-carotene from fresh and dehydrated drumstick leaves (Moringa oleifera) in a rat model. Plant Foods Hum Nutr 2001;56:83–95.

  • 13.

    Sreelatha S, Padma PR. Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods Hum Nutr 2009;64:303–11.

  • 14.

    Singh BN, Singh BR, Singh RL, Prakash D, Dhakarey R, Upadhyay G, et al. Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem Toxicol 2009;47:1109–16. [Crossref] [PubMed] [Web of Science]

  • 15.

    Atawodi SE, Atawodi JC, Idakwo GA, Pfundstein B, Haubner R, Wurtele G, et al. Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J Med Food 2010;13:710–6. [Crossref] [PubMed]

  • 16.

    Valerio LG Jr, Arvidson KB, Busta E, Minnier BL, Kruhlak NL, Benz RD. Testing computational toxicology models with phytochemicals. Mol Nutr Food Res 2010;54:186–94. [PubMed] [Web of Science] [Crossref]

  • 17.

    Ambi AA, Abdurahman EM, Umar A, Katsayal UA, Sule MI, Pateh UU, et al. Toxicity evaluation of Moringa oleifera leaves. Int J Pharmaceut Res Innovat 2011;4:22–4.

  • 18.

    Paul CW, Didia BC. The effect of methanolic extract of Moringa oleifera lam roots on the histology of kidney and liver of guinea pigs. Asian J Med Sci 2012;4:55–60.

  • 19.

    Kavitha C, Ramesh M, Kumaran SS, Lakshmi SA. Toxicity of Moringa oleifera seed extract on some hematological and biochemical profiles in a freshwater fish, Cyprinus carpio. Exp Toxicol Pathol 2012;64:681–7. [Web of Science] [Crossref]

  • 20.

    Awodele O, Oreagba IA, Odoma S, da Silva JA, Osunkalu VO. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol 2012;139:330–6. [Web of Science] [Crossref]

  • 21.

    Asare GA, Gyan B, Bugyei K, Adjei S, Mahama R, Addo P, et al. Toxicity potentials of the nutraceutical Moringa oleifera at supra-supplementation levels. J Ethnopharmacol 2012;139:265–72. [Crossref] [Web of Science] [PubMed]

  • 22.

    Rolim LA, Macêdo MF, Sisenando HA, Napoleão TH, Felzenszwalb I, Aiub CA, et al. Genotoxicity evaluation of Moringa oleifera seed extract and lectin. J Food Sci 2011;76:T53–8. [PubMed] [Crossref]

  • 23.

    Chivapat S, Sincharoenpokai P, Saktiyasuthorn N, Shuaprom A, Thongsrirak P, Sakpetch A, et al. Acute and chronic toxicity of Moringa oleifera Linn leaves extracts. Thai J Vet Med 2011;41:417–24.

  • 24.

    Adedapo AA, Mogbojuri OM, Emikpe BO. Safety evaluations of the aqueous extract of the leaves of Moringa oleifera in rats. J Med Plants Res 2009;8:586–91.

  • 25.

    Public Health Service (PHS). Public health service policy on humane care and the use of laboratory animals. Washington, DC: US Department of Health and Human services, 1996:99–158.

  • 26.

    Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem 1949;177:751–66. [PubMed]

  • 27.

    Steven CK. Alanine and aspartate aminotransferase, principle and usage. In: James JT, Jenifer R, editors. Liver function. Clinical chemistry theory, analysis and correlation, 3rd ed. London: M. Mosby, 1996:504–27.

  • 28.

    Gustafsson JE. Improved specificity of serum albumin determination and estimation of acute phase reactant by the use of bromocresol green reaction. Clin Chem 1976;22:616–22. [PubMed]

  • 29.

    Marsh WH, Fingerhut B, Miller H. Automated and manual direct methods for the determination of blood urea. Clin Chem 1965;11:624–7. [PubMed]

  • 30.

    Biod T, Sirota B. In: Watson A, editor. Practical clinical biochemistry. New Delhi: Prentice-Hall of India Private Ltd., 1948:142–5.

  • 31.

    Fraunfelder FW. Ocular side effects from herbal medicines and nutritional supplements. Am J Ophthalmol 2004;138:639–47. [Crossref] [PubMed]

  • 32.

    Sudha P, Asdaq SM, Dhamingi SS, Chandrakala GK. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in animals. Indian J Physiol Pharmacol 2010;54:133–40. [PubMed]

  • 33.

    Manaheji H, Jafari S, Zaringhalam J, Rezazadeh S, Taghizadfarid R. Analgesic effects of methanolic extracts of the leaf or root of Moringa oleifera on complete Freund’s adjuvant-induced arthritis in rats. Zhong Xi Yi Jie He Xue Bao 2011;9:216–22.

  • 34.

    Gupta A, Gautam MK, Singh RK, Kumar MV, Rao ChV, Goel RK, et al. Immunomodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice. Indian J Exp Biol 2010;48:1157–60. [PubMed]

  • 35.

    Mahajan SG, Mehta AA. Immunosuppressive activity of ethanolic extract of seeds of Moringa oleifera Lam. in experimental immune inflammation. J Ethnopharmacol 2010;130:183–6. [Web of Science] [Crossref]

  • 36.

    Ashok Kumar BS, Lakshman K, Arun Kumar PA, Viswantha GL, Veerapur VP, Thippeswamy BS, et al. Hepatoprotective activity of methanol extract of Amaranthus caudatus Linn against paracetamol-induced hepatic injury in rats. Zhong Xi Yi Jie He Xue Bao 2011;9:194–200.

  • 37.

    Soumendra D, Abhijit B, Shyamaprasad C. Ameliorative effect of Livina, a polyherbal preparation on Diclofenac-induced liver injury: a comparison with Silymarin. J Pharm Res 2010;3:2794–8.

  • 38.

    Nkosi CZ, Opoku AR, Terblanche SE. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4-induced liver injury in low protein feed rats. Phytother Res 2005;19:341–5. [Crossref]

  • 39.

    Sharma MK, Kumar M, Kumar A. Ocimum sanctum aqueous leaf extract provides protection against mercury induced toxicity in Swiss albino mice. Indian J Exp Biol 2002;40:1079–82. [PubMed]

  • 40.

    Tessari P, Sofia A, Saffioti S, Vettore M, Verzola D, Millioni R, et al. Effects of chronic metabolic acidosis on splanchnic protein turnover and oxygen consumption in human beings. Gastroenterology 2010;138:1557–65. [Web of Science] [PubMed] [Crossref]

  • 41.

    Workeneh BT, Mitch WE. Review of muscle wasting associated with chronic kidney disease. Am J Clin Nutr 2010;91:1128S–32S. [Crossref]

  • 42.

    Mayne PD. The kidneys and renal calculi. In: Clinical chemistry in diagnosis and treatment, 6th ed. London: Edward Arnold Publications, 1994:2–24.

  • 43.

    Ali BH, Ben Ismail TH, Basheer AA. Sex related differences in the susceptibility of rat to gentamicin nephrotoxicity: influence of gonadectomy and hormonal replacement therapy. Ind J Pharmacol 2001;33:369–73.

  • 44.

    Flaoyen A, Hove K, Wilkins AL. Tolerance to nephrotoxic component of Narthecium ossifragum in sheep: the effects of repeated oral doses of plant extracts. Vet Res Commun 2001;25:127–36. [Crossref] [PubMed]

About the article

Corresponding author: Ademola A. Oyagbemi, Faculty of Veterinary Medicine, Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Oyo State, Nigeria, Phone: +2348033639776, Fax: +234028103043, E-mail:


Received: 2012-10-10

Accepted: 2013-02-24

Published Online: 2013-03-18

Published in Print: 2013-11-01


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2012-0061. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Sidney J. Stohs and Michael J. Hartman
Phytotherapy Research, 2015, Volume 29, Number 6, Page 796

Comments (0)

Please log in or register to comment.
Log in